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Analysis of Human T-Cell Antigen Receptor Variable β Gene Usage Following Vaccination with Recombinant HBsAg

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Abstract

We analyzed the TcR Vβ gene usage beforeand after vaccination with the hepatitis B vaccine sincechanges in the TcR Vβ gene families would beconsidered to provide preliminary evidence of amechanism to prevent HBV infection. Six healthy adultvolunteers received immunizations. TcR Vβ usage,T-cell proliferation, and HLA class II alleles wereexamined in peripheral blood mononuclear cells (PBMC) both before and after vaccination. Furthermore,TcR Vβ usage in postimmunization PBMC was alsocompared with PBMC cultured with recombinant HBsAg(rHBsAg). The level of in vitro T-cell proliferation in the presence of rHBsAg increasedsignificantly (P < 0.01) in PBMC isolated aftervaccinations. Increases in the different TcR Vβgenes were also observed in each individual followingvaccinations, regardless of the similarity in their HLAalleles. Specific HBV-related antigen-responsive T cellswere induced after HB vaccination, without any commonrestriction for the TcR Vβ gene families. The mechanism that helps prevent HBV infection wasthus found to involve multiclonal alterations in the TcRVβ repertoire.

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Authors
  1. Kouichi Yuh
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  2. Shinya Sugyo
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  3. Ken Nakamura
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  4. Hiroshi Shijo
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  5. Kanako Emi
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  6. Kyoko Harada
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  7. Saeko Yoshitake
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  8. Nobuhiro Kimura
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  9. Toyoki Moribe
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  10. Toshihiko Kaneshige
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  11. Makoto Okumura
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Yuh, K., Sugyo, S., Nakamura, K. et al. Analysis of Human T-Cell Antigen Receptor Variable β Gene Usage Following Vaccination with Recombinant HBsAg. Dig Dis Sci 43, 880–886 (1998). https://doi.org/10.1023/A:1018846921408

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