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In Vitro Wound Repair by Human Gastric Fibroblasts: Implications for Ulcer Healing

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Abstract

Fibroblasts modulate epithelial biologicalactivities and play a key role in the ulcer healingprocess. There is no information regarding thebiological response of human gastric fibroblasts toregulatory compounds. The aim of this study was to assessthe effects of growth factors and prostaglandins on anin vitro model of human gastric fibroblast wound repair.Subconfluent fibroblast cultures were used to study proliferative responses, determined by[3H]thymidine incorporation into DNA. Invitro wound repair was determined in confluentfibroblast monolayers after mechanical denudation. Thepresence of putative growth factors secreted by fibroblastswas studied in conditioned medium by heparin-affinitychromatography and immunodetection with specificantibodies. Serum and platelet-derived growth factor (PDGF)-BB induced a dramatic increase in bothgastric fibroblast proliferation and closure of woundedcell monolayers, whereas these activities were inhibitedby both transforming growth factor(TGF)-β1 and prostaglandin E1. Basalactivities in unstimulated gastric fibroblasts werelower than those obtained in skin fibroblasts.Conditioned medium stimulated fibroblast proliferationand wound repair activity, which was inhibited by the addition of suramin,and was partially dependent on the presence of PDGF-likefactor. PDGF is a major, autocrine promotor of humangastric fibroblast-dependent wound repair activities, which are inhibited by prostaglandins andTGF-β. These findings might be important for futuretherapeutic ulcer healing approaches.

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Authors
  1. E. Piazuelo
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  2. A. Lanas
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  3. P. Jimenez
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  4. A. Garcia-Gonzalez
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  5. F. Esteva
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Piazuelo, E., Lanas, A., Jimenez, P. et al. In Vitro Wound Repair by Human Gastric Fibroblasts: Implications for Ulcer Healing. Dig Dis Sci 43, 1230–1240 (1998). https://doi.org/10.1023/A:1018803707179

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  • DOI: https://doi.org/10.1023/A:1018803707179

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