Abstract
To evaluate the effects of glutaraldehyde treatment at different disinfection temperatures, Atlantic halibut, Hippoglossus hippoglossus (L.), and turbot, Scophthalmus maximus (L.), eggs, incubated at 5 and 12°C, respectively, were disinfected with 400–1200 mg glutaraldehyde l-1 at three different contact times (2.5, 5 and 10 min). Egg batches of both poor and good quality were tested for halibut. Positive effects were more pronounced in poor than in good-quality batches at hatching. Egg disinfection had a highly positive effect on the viability of yolk-sac larvae in both types of batches. A level between 400 and 800 mg l-1 at a contact time of 5–10 min was optimal for halibut: at lower levels, the bactericidal effect was reduced, and at higher levels, there were indications of toxic effects. Halibut eggs disinfected with optimal doses of glutaraldehyde had furthermore a reduced hatching time and more synchronous hatching as compared with untreated eggs. Turbot was more sensitive to higher doses than halibut, and the best larval performance was obtained for 400–800 mg glutaraldehyde l-1 at a contact time of 2.5 min. A further evaluation should, however, be performed before recommendations are given for species incubated at temperatures higher than 5°C.
Similar content being viewed by others
REFERENCES
Block, S.S. (1977) Disinfection, Sterilization and Preservation (2nd edition). Leax Febiger: Philadelphia, 1049 pp.
Borick, P.M. (1968) Chemical sterilizers (chemosterilizers). Advances in Applied Microbiology 10, 291–312.
Gardner, J.F. and Peel, M.M. (1986) Introduction to Sterilization and Disinfection. Churchill Livingstone: Melbourne, 183 pp.
Gorman, S.P., Scott, E.M. and Russel, A.D. (1980) Antimicrobial activity, uses and mechanism of action of glutaraldehyde. Journal of Applied Bacteriology 48, 161–190.
Harboe, T., Huse, I. and Øie, G. (1994) Effects of egg disinfection on yolk sac and first feeding stages of halibut (Hippoglossus hippoglossus L.) larvae. Aquaculture 119, 157–165.
McFadden, T.W. (1969) Effective disinfection of trout eggs to prevent egg transmission of Aeromonas liquefaciens. Journal of the Fisheries Research Board of Canada 26, 2311–2318.
Salvesen, I. and Vadstein, O. (1995) Surface disinfection of eggs from marine fish: evaluation of four chemicals. Aquaculture International 3, 1–18.
Schachte, J.H. (1979) Iodophor disinfection of muskellunge eggs under intensive culture in hatcheries. The Progressive Fish-Culturist 41, 189–190.
Subasinghe, R.P. and Sommerville, C. (1985) Disinfection of Oreochromis mossambicus (Peters) eggs against commonly occurring potentially pathogenic bacteria and fungi under artificial hatchery conditions. Aquaculture and Fisheries Management 16, 121–127.
Vadstein, O., Øie, G., Olsen, Y., Salvesen, I. and Skjermo, J. (1993) A strategy to obtain microbial control during larval development of marine fish. In: Fish Farming Technology — Proceedings of the First International Conference on Fish Farming Technology (eds H. Reinertsen, L.A. Dahle, L. Jørgensen and K. Tvinnereim) Balkema: Rotterdam, pp. 67–75.
Zar, H.J. (1984) Biostatistical Analysis (2nd edition). Prentice-Hall International Editions: Englewood, Cliffs, New Jersey, 718 pp.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Salvesen, I., Oie, G. & Vadstein, O. Surface disinfection of Atlantic halibut and turbot eggs with glutaraldehyde: evaluation of concentrations and contact times. Aquaculture International 5, 249–258 (1997). https://doi.org/10.1023/A:1018343602872
Issue Date:
DOI: https://doi.org/10.1023/A:1018343602872