Skip to main content
SpringerLink
Log in

Combination of resistance to potato late blight in foliage and tubers by intraspecific dihaploid protoplast fusion

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Five dihaploid Solanum tuberosum genotypes, encompassing different levels of polygenically inherited resistance to potato late blight disease (Phytophthora infestans (Mont.) de Bary) in foliage and tubers, were used in four intraspecific protoplast fusion combinations. Vigorous growing putative hybrid calli were selected four weeks after electrofusion. Intact plants were regenerated from 6% of the selected calli. Verification of hybridity was accomplished by use of RAPD analysis which revealed that 53% of the regenerated plants were true somatic hybrids. The score of true somatic hybrids in the different fusion combinations ranged within 21% – 100%. The hybrid plants were analysed for resistance to foliage blight in a field trial and assessed for tuber blight resistance by use of a laboratory test. Resistance to late blight in foliage and tubers varied between the hybrids. Very high levels of resistance to both foliage and tuber blight were obtained in some hybrids. However, loss of resistance in some hybrids as compared to the parental plants were also observed. Possible reasons for the phenotypic disappearance of resistance to either foliage or tuber blight are suggested.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Cooper-Bland, S., M.J. De Maine, M.L.M.H. Flemming, M.S. Phillips, W. Powell & A. Kumar, 1994. Synthesis of intraspecific somatic hybrids of Solanum tuberosum: assessments of morphological, biochemical and nematode (Globodere pallida) resistance characteristics. Jour of Exp Bot 45: 1319-1325.

    CAS  Google Scholar 

  • Cruickshank, G., H.E. Stewart & R.L. Wastie, 1982. An illustrated assessment key for foliage blight of potatoes. Potato Res 25: 213-214.

    Article  Google Scholar 

  • De Maine, M.J., 1978. Field resistance to late blight and potato root eelworm in Group Tuberosum dihaploids. Euphytica 27: 305-17.

    Article  Google Scholar 

  • Hutten, R.C.B., E.J.M.M. Scholberg, D.J. Huigen, J.G. Th Hermsen & E. Jacobsen, 1994. Analysis of dihaploid induction and production ability and seed parent x pollinator interaction in potato. Euphytica 72: 61-64.

    Article  Google Scholar 

  • Hutten, R.C.B., W.J.J. Soppe, J.G. Th. Hermsen & E. Jacobsen, 1995. Evaluation of dihaploid populations from potato varieties and breeding lines. Potato Res 38: 77-88.

    Article  Google Scholar 

  • Johansson, L., 1986. Improved methods for induction of embryogenesis in anther cultures of Solanum tuberosum. Potato Res 29: 179-190.

    Article  Google Scholar 

  • Kumar, A. 1994. Somaclonal variation in potato. In: J.E. Bradshaw & G.R. MacKay (Eds), Potato genetics. CAB International: pp. 197-212. ISBN 0851988695.

  • Leonard-Schippers, C., W. Gieffers, R. Schäfer-Pregl, E. Ritter, S.J. Knapp, F. Salamini, & C. Gebhardt, 1994. Quantitative resistance to Phytophtora infestansin potato: a case study of QTL mapping in an allogamous plant species. Genetics 137: 67-77.

    Google Scholar 

  • Liu, C.-A. & D.S. Douches 1993. Production of haploids of potato (Solanum tuberosumsubsp. tuberosum) and their identification with electrophoretic analysis. Euphytica 70: 113-126.

    Article  Google Scholar 

  • Mackay, G.R. & P. Gregory, 1996. An agenda for future potato research. EAPR1996. 13th Triennial Conference of the European Association for Potato Research, Veldhoven, The Netherlands. Abstracts pp: 6-9.

  • Möllers, C. & G. Wenzel, 1992. Somatic hybridization of dihaploid potato protoplasts as a tool for potato breeding. Botanica-Acta 105: 133-139.

    Google Scholar 

  • Nagy, J.I.& P. Maliga, 1976. Callus induction and plant regeneration from mesophyll protoplasts of Nicotiana sylvestris. Z Pflanzenphysiol 78: 453-455.

    Google Scholar 

  • Pan, W.H., A. Houben & R. Schlegel, 1993. Highly effective cell synchronization in plant roots by hydroxyurea aminoprophosmethyl or colchicine. Genome 36: 387-390.

    CAS  PubMed  Google Scholar 

  • Rasmussen, J.O. & O.S. Rasmussen, 1995. Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis. Physiologia Plantarum 93: 357-364.

    Article  CAS  Google Scholar 

  • Rasmussen, J.O., J.P. Nepper & O.S. Rasmussen, 1996. Analysis of somatic hybrids between two sterile dihaploid Solanum tuberosumL. breeding lines. Restoration of fertility and complementation of G.pallidaPa2 and Pa3 resistance. Theor Appl Genet 92: 403-410.

    Article  Google Scholar 

  • Ross, H., 1986. Potato Breeding - Problems and Perspectives. Verlag Paul Parey, Berlin und Hamburg, pp. 82-86. ISBN 3-489-61110-1.

    Google Scholar 

  • Shaner, G. & R.E. Finney, 1977. The effect of Nitrogen fertilization on the expression of slow mildewing resistance on knox wheat. Phytopathology 67: 1051-1056.

    Article  CAS  Google Scholar 

  • Sopory, S.K. & Y.P.S. Bajaj, 1987. Anther culture and haploid production in potato. Biotechnology in Agriculture and Forestry, Vol. 3, Potato (Y.P.S. Bajaj) (Ed) Springer Verlag, pp. 89-105.

  • Sree Ramulu, K., P. Dijkhuis, S. Roest, G.S. Bokelmann & B. De Groot, 1986. Variation in the phenotype and chromosome number of plants regenerated from protoplasts of dihaploid and tetraploid potato. Plant Breeding 97: 119-128.

    Article  Google Scholar 

  • Stewart, H.E., R.L. Wastie, J.E. Bradshaw & J. Brown, 1992. Inheritance of resistance to late blight in foliage and tubers of progenies from parents differing in resistance. Potato Res 35: 313-319.

    Article  Google Scholar 

  • Thach, N.Q., U. Frei & G. Wenzel, 1993. Somatic fusion for combining virus resistances in Solanum tuberosumL. Theor Appl Genet 85: 863-867.

    Article  Google Scholar 

  • Umaerus, V. & M. Umaerus, 1994. Inheritance of resistance to late blight. In: J.E. Bradshaw and G. R. Mackay (Eds), Potato Genetics. CAB International, UK, pp. 365-401. ISBN 0851988695.

    Google Scholar 

  • Waara, S., L. Pijnacker, M. Ferwerda, A. Wallin & T. Eriksson, 1992. A cytogenetic and phenotypic characterization of somatic hybrid plants obtained after fusion of 2 different dihaploid clones of potato (Solanum tuberosumL.). Theor Appl Genet 85: 470-479.

    Article  Google Scholar 

  • Wastie, R.L., 1991. Breeding for resistance. In: D.S. Ingram, P.H. Williams (Eds) Advances in Plant Pathology, Vol 7: Phytophtora infestans, the cause of late blight in potato. Academic Press, London, pp. 193-223.

    Google Scholar 

  • Zandstra, H.G., 1996. Twenty-five years of international potato research, a retrospective and forward look. Potato Research 39: 395-401.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular and Structural Biology, Aarhus University, C.F. Møllers Allé 130, DK-8000, Aarhus, Denmark

    John O. Rasmussen & Ole S. Rasmussen

  2. Breeding station, Danish Potato Breeding Foundation, Grindstedvej 55, DK-7184, Vandel, Denmark

    Jens Peter Nepper, Hanne-Grete Kirk & Karl Tolstrup

Authors
  1. John O. Rasmussen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jens Peter Nepper
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hanne-Grete Kirk
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Karl Tolstrup
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ole S. Rasmussen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rasmussen, J.O., Nepper, J.P., Kirk, HG. et al. Combination of resistance to potato late blight in foliage and tubers by intraspecific dihaploid protoplast fusion. Euphytica 102, 363–370 (1998). https://doi.org/10.1023/A:1018302103100

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1018302103100

Search

Navigation