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The ability of selected bacterial isolates to utilise components of synthetic metal-working fluids as sole sources of carbon and nitrogen for growth

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Abstract

A total of 24 bacterial isolates able to grow on metal-working fluids were obtained from soil or metal-working fluids (both in-use and heavily contaminated fluids). Pure cultures of the isolates were tested for their ability to degrade a selection of components, including borate esters, phosphate ester, biocide and triethanolamine, typically found in synthetic metal-working fluids. All components, when present at a level equivalent to half that found in an in-use metal-working fluid, supported growth when utilised as the sole source of carbon and/or nitrogen. Each component was degraded by at least 50% by an individual isolate within 120 hours in batch liquid culture.

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References

  • Bauchop, T. & Elsden, S. R. (1960). J. Gen. Microbiol. 25: 457–469.

    Google Scholar 

  • Bennett, E. O. (1972). Lub. Eng. 28: 237–247.

    Google Scholar 

  • Cox, D. P. (1978), Adv. Appl. Microbiol. 23: 173–194.

    Google Scholar 

  • Dojlido, J. & Best, G. A. (1993) Chemistry of Water and Water Pollution. Ellis Horwood, New York, USA.

    Google Scholar 

  • Fawcett, J. K., & Scott, J. E. (1960). J. Clin. Path. 13: 156–159.

    Google Scholar 

  • Foxall-VanAken, S., Brown, J. A., Young, W., Salmeen, I., McClure, T., Napier, S. & Olsen, R. H. (1986). Appl. Environ, Microbiol. 51: 1165–1169.

    Google Scholar 

  • Greenberg, A. E., Clesceri, L. S. & Eaton, A. D. (1992) Standard Methods for the Examination of Waste and Waste-water. 18th Edition. APHA, AWWA, WEF, Washington, USA.

    Google Scholar 

  • Hill, E. C. (1976) Some aspects of microbial degradation of aluminium rolling coolants. In Proceedings of the Third International Biodegradation Symposium. (Eds. J. M. Sharpley & A. M. Kaplan) pp 243–258. Applied Science Publishers, London, UK.

    Google Scholar 

  • Mattsby-Baltzer, I., Sandin, M. Ahlstrom, B., Allenmark, S., Edebo, M., Falsen, E., Pedersen, K., Rodin, N., Thompson, R. A. & Edebo, L. (1989). Appl. Env. Microbiol. 55: 2681–2689.

    Google Scholar 

  • Miller, J. H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, New York, USA.

    Google Scholar 

  • Napier, S., Cattell, H. G. & Coffin, P. S. (1988). Synthetic Lubrication. 5: 171–184.

    Google Scholar 

  • Prince, E. L. & Morton, L. H. G. (1988) Fungal Biodeterioration of Synthetic Metal Working Fluids. In Biofilms. Proceedings of the Spring Meeting of the Biodeterioration Society. (Ed. L. H. G. Morton & A. H. L. Chamberlain) pp. 107–122. Lancashire Polytechnic Publications Service, Preston, UK.

    Google Scholar 

  • Pyziak, T., & Brinkman, D. W. (1993). Lub. Eng. May 93: 339–346.

    Google Scholar 

  • Rossmoore, H. W. & Holtzman, G. H. (1974). Dev. Ind. Microbiol. 15: 273–280.

    Google Scholar 

  • Williams, G. R. & Calley, A. G. (1982) J. Gen. Microbiol. 128: 1203–1210.

    Google Scholar 

  • Wright, M. A., Taylor, F., Randles, S. J., Brown, D. E. & Higgins, I. J. (1993) Appl. Env. Microbiol. 59: 1072–1076.

    Google Scholar 

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Authors and Affiliations

  1. Research School of Biosciences, University of Kent, Canterbury, Kent, UK

    K.L.M. Buers & C.J. Knowles

  2. Microlan Consultancy Services, University of Central Lancashire, Preston, UK

    E.L. Prince

Authors
  1. K.L.M. Buers
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  2. E.L. Prince
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  3. C.J. Knowles
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Buers, K., Prince, E. & Knowles, C. The ability of selected bacterial isolates to utilise components of synthetic metal-working fluids as sole sources of carbon and nitrogen for growth. Biotechnology Letters 19, 791–794 (1997). https://doi.org/10.1023/A:1018300628816

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