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Nucleolar Organizing Regions, 18S and 5S rDNA in Astyanax Scabripinnis (Pisces, Characidae): Populations Distribution and Functional Diversity

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Abstract

Astyanax scabripinnis specimens from four distinct populations in Brazil were studied with respect to their karyotype macrostructure, nucleolar organizer regions, and 18S and 5S rRNA genes. The four populations showed a 2n = 50 chromosomes (3 M + 11 SM + 5 ST + 6 A pairs) and 1–2 B chromosomes. No chromosomal differentiations were observed between sexes. Although a karyotypic diversity has been characterized in this fish group, the populations now analyzed presented the same macrokaryotypic pattern. Chromosome mapping of 5S rDNA showed a total of eight sites located in four distinct chromosomal pairs, with no apparent differences among populations. A comparative study on 18S rDNA locations and Ag-NORs showed some secondary NOR sites that are not usually expressed in karyotypes and a probable differential NOR activity among populations. Correlations between these data, environmental conditions and B chromosomes are discussed.

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Authors and Affiliations

  1. Departamento de Gene Evolucção, Universidade Federal de São Carlos, Caixa Postal 676, 13565-905, São Carlos, SP, Brazil

    Dagmar Aparecida de Marco Ferro, Daniela Morilha Néo, Orlando Moreira-Filho & Luiz Antonio Carlos Bertollo

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  1. Dagmar Aparecida de Marco Ferro
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  2. Daniela Morilha Néo
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  3. Orlando Moreira-Filho
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  4. Luiz Antonio Carlos Bertollo
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de Marco Ferro, D.A., Néo, D.M., Moreira-Filho, O. et al. Nucleolar Organizing Regions, 18S and 5S rDNA in Astyanax Scabripinnis (Pisces, Characidae): Populations Distribution and Functional Diversity. Genetica 110, 55–62 (2000). https://doi.org/10.1023/A:1017963217795

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