Abstract
Cultures of lymphoblastoid Namalva cells have been used to check the absence of toxic impurities in fluorocarbons destined for biomedical applications. The test comprises the measurement of growth rate and viability in the presence of the fluorocarbon, and of growth after reseeding. It was used to establish a procedure for the detoxification of a family of fluorocarbons by evaluating the effect on toxicity of a series of individual or combined treatments. A convenient, rapid and industrially feasible procedure was developed which includes washing with 10 % aqueous KOH, shaking with charcoal and filtration over alumina.
Similar content being viewed by others
Literature
Le Blanc, M., Riess, J. G. (1982) in Preparation, Properties, and Industrial Applications of Organofluorine Compounds (R. E. Banks ed.), pp. 85–138, Ellis Horwood, Chichester. Riess, J. G., Le Blanc, M. (1982) Pure & Appl. Chem. 54, 2383–2406. Riess, J. G., Follana, R. (1984) Rev. Fr. Transfusion et Immuno-hématol. 27 191–226.
Riess, J. G. (1984) Artif. Organs 8, 44–56.
Geyer, R. P. (1979) in Proc. IVth Int. Symp. Perfluorochem. Blood Substitutes (Kyoto, 1978). Excerpta Medica, Amsterdam, 3–32.
Advances in Blood Substitute Research (1983) (Bolin, R. B., Geyer, R. P., Nemo, G. J. eds.) Alan R. Liss, New York. (Progr. Clin. Biol. Res. 122).
Moore, R. E., Clark, L. C., Miller, M. L. (1979) in Proc. IVth Int. Symp. Perfluorochem. Blood Substitutes (Kyoto, 1978). Excerpta Medica, Amsterdam, 69–79. Yokoyama, K., Fukaya, C., Tsuda, Y., Suyama, T., Naito, R., Yamanouchi, K., Scherer, K. V. (1982) in Abstracts Symp. Organofluorine Compounds Med. Biol. (A. C. S. Meeting, Las Vegas, March 1982).
Riess, J. G., Le Blanc, M. (1978) Angew. Chem. Intl. Ed. 17, 621–634. Jeanneaux, F., Le Blanc, M., Poggi, D., Follana, R., Riess, J. G., Ref. 4 pp. 442. Jeanneaux, F., Le Blanc, M., Riess, J. G., Yokoyama, K. (1984) Nouv. J. Chim. 8, 251–257.
Mizrahi, A. (1981) in Methods in Enzymology, Academic Press, London, 78, 54–68. Bodo, G. (1981) ibid. 78, 69–75.
Grec, J. J., Devallez, B., Marcovich, H., Riess, J. G. (1982) Colloque Nat. de Génie Biol. et Méd., Toulouse. Marcovich, H. (1983) Fr. Patent No 25 13 264
Grec, J. J., Riess, J. G., Devallez, B. (1985) Nouv. J. Chim. 9, 109–117.
Seiyaku, T. (1983) Fr. Pat. No 2 177 051.
Klein, G., Dombos, L., Gothoskar, B. (1972) Int. J. Cancer 10, 44–57.
Goldman, J. M., Th'ng, K. H., Park, D. S., Spiers, A. S. D., Lowenthal, R. M., Ruutu, T. (1978). Brit. J. Haematol. 40, 185–195.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Le Blanc, M., Riess, J.G., Poggi, D. et al. Use of Lymphoblastoid Namalva Cell Cultures in a Toxicity Test. Application to the Monitoring of Detoxification Procedures for Fluorocarbons to be used as Intravascular Oxygen-Carriers. Pharm Res 2, 246–248 (1985). https://doi.org/10.1023/A:1016325130406
Issue Date:
DOI: https://doi.org/10.1023/A:1016325130406