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Characterization of acclimation of Hordeum vulgare to high irradiation based on different responses of photosynthetic activity and pigment composition

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Abstract

The ability of spring barley (Hordeum vulgare cv. Akcent) to adjust the composition and function of the photosynthetic apparatus to growth irradiances of 25–1200 μmol m−2 s−1 was studied by gas exchange and chlorophyll a fluorescence measurements and high-performance liquid chromatography. The increased growth irradiance stimulated light- and CO2-saturated rates of CO2 assimilation expressed on a leaf area basis up to 730 μmol m−2 s−1 (HL730), whereas at an irradiance of 1200 μmol m−2 s−1 (EHL1200) both rates decreased significantly. Further, the acclimation to EHL1200 was associated with an extremely high chlorophyll a/b ratio (3.97), a more than doubled xanthophyll cycle pool (VAZ) and a six-fold higher de-epoxidation state of the xanthophyll cycle pigments as compared to barley grown under 25 μmol m−2 s−1 (LL25). EHL1200 plants also exhibited a long-term inhibition of Photosystem II (PS II) photochemical efficiency (F v/F m). Photosynthetic capacity, chlorophyll a/b and VAZ revealed a linear trend of dependence on PS II excitation pressure in a certain range of growth irradiances (100–730 μmol m−2 s−1). The deviation from linearity of these relationships for EHL1200 barley is discussed. In addition, the role of increased VAZ and/or accumulation of zeaxanthin and antheraxanthin in acclimation of barley to high irradiance is studied with respect to regulation of non-radiative dissipation and/or photochemical efficiency within PS II.

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Authors and Affiliations

  1. Department of Physics, Ostrava University, Faculty of Science, 30. dubna 22, 701 03, Ostrava 1, Czech Republic

    Irena Kurasová, Martin Čajánek, Jiří Kalina & Vladimír Špunda

  2. University of South Bohemia, Branišovská 31, 370 05, České Budějovice, Czech Republic

    Martin Čajánek & Jiří Kalina

  3. Laboratory of Ecological Physiology of Forest Trees, Institute of Landscape Ecology, Academy of Sciences of the Czech Republic, Poříčí 3b, 603 00, Brno, Czech Republic

    Otmar Urban

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  1. Irena Kurasová
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  2. Martin Čajánek
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  5. Vladimír Špunda
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Correspondence to Vladimír Špunda.

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Kurasová, I., Čajánek, M., Kalina, J. et al. Characterization of acclimation of Hordeum vulgare to high irradiation based on different responses of photosynthetic activity and pigment composition. Photosynthesis Research 72, 71–83 (2002). https://doi.org/10.1023/A:1016018900535

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