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Production of S-adenosyl- L-methionine by a mutant strain of Kluyveromyces lactis

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Abstract

S-Adenosyl-l-methionine (AdoMet) was produced by a mutant strain Kluyveromyces lactis AM-65 grown on whey. A full factorial design method of three factors – (NH4)2SO4 (factor x 1), corn steep liquor (factor x 2) and l-methionine (factor x 3) on three levels – was used to determine the optimal medium conditions for the production of AdoMet. A time course shake-flask experiment in optimal whey medium (x 1=3.1 g l−1, x 2=12.7 g l−1, x 3=4.6 g l−1) was also carried out and the results confirmed the results of the factorial design and subsequent quadratic modelling and optimization of AdoMet production which reached 90 mg g−1 cell dry wt.

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Authors and Affiliations

  1. Technological College – Razgrad, University of Rousse, P.O. Box 110, 3 Aprilsko Vastanie Blvd., 7200, Razgrad, Bulgaria

    Kremena Mincheva & Veselka Kamburova

  2. University of Sofia, Faculty of Biology, Sofia, Bulgaria

    Venelin Balutzov

Authors
  1. Kremena Mincheva
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  2. Veselka Kamburova
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  3. Venelin Balutzov
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Mincheva, K., Kamburova, V. & Balutzov, V. Production of S-adenosyl- L-methionine by a mutant strain of Kluyveromyces lactis . Biotechnology Letters 24, 985–988 (2002). https://doi.org/10.1023/A:1015604725893

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  • DOI: https://doi.org/10.1023/A:1015604725893

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