Photosynthetica 2001, 39(4):553-556 | DOI: 10.1023/A:1015604128832

Three Substrate Binding Sites on Spinach Ferredoxin:NADP+ Oxidoreductase. Studies with Selectively Acting Inhibitors

M. Bojko, S. Więckowski

The effects of phenylmercuric acetate (PMA) and apoferredoxin (apoFd) on the diaphorase activity of spinach ferredoxin:NADP+ oxidoreductase (FNR) in the presence of dibromothymoquinone (DBMIB) or cytochrome c (Cyt c) were studied. PMA inhibited effectively (I50 = < 5 μM) ferredoxin-dependent Cyt c reduction but did not affect evidently the enzyme activity in the presence of DBMIB as an electron acceptor. ApoFd caused also inhibition of Cyt c reduction but slightly stimulated, like ferredoxin, DBMIB reduction. We confirm a hypothesis according to which three binding sites for substrates [NADP(H), Fd-Cyt c, quinone/dichlorophenol indophenol] occur within the molecule of isolated FNR.

Additional key words: apoferredoxin; cytochrome c; diaphorase activity; dibromothymoquinone; ferredoxin; phenylmercuric acetate

Published: December 1, 2001  Show citation

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Bojko, M., & Więckowski, S. (2001). Three Substrate Binding Sites on Spinach Ferredoxin:NADP+ Oxidoreductase. Studies with Selectively Acting Inhibitors. Photosynthetica, 39(4), 553-556. doi: 10.1023/A:1015604128832
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