Skip to main content
SpringerLink
Log in

Kinetics of electron transfer between soluble cytochrome c-554 and purified reaction center complex from the green sulfur bacterium Chlorobium tepidum

  • Published:
Photosynthesis Research Aims and scope Submit manuscript

Abstract

Soluble cytochrome c-554 (M r ∼ 10 kDa) is purified from the green sulfur bacterium Chlorobium tepidum. Its midpoint redox potential is determined to be +148 mV from redox titration at pH 7.0. The kinetics of cytochrome c-554 oxidation by a purified reaction center complex from the same organism were studied by flash absorption spectroscopy at room temperature, and the results indicate that the reaction partner of cytochrome c-554 is cytochrome c-551 bound to the reaction center rather than the primary donor P840. The second-order rate constant for the electron donation from cytochrome c-554 to cytochrome c-551 was estimated to be 1.7×107 M−1 s−1. The reaction rate was not significantly influenced by the ionic strength of the reaction medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Albouy D, Sturgis JN, Feiler U, Nitschke W and Robert B (1997) Membrane-associated c-type cytochromes from the green sulfur bacterium Chlorobium limicola forma thiosulfatophilum: purification and characterization of cytochrome c553. Biochemistry 36: 1927–1932

    Article  PubMed  Google Scholar 

  • Bartsch RG (1978) Cytochromes. In: Clayton RK and Sistrom WS (eds) The Photosynthetic Bacteria, pp 249–279. Plenum Press, New York/London

    Google Scholar 

  • Brettel K (1997) Electron transfer and arrangement of the redox cofactors in Photosystem I. Biochim Biophys Acta 1318: 322–373

    Google Scholar 

  • Evans MCW and Buchanan BB (1965) Photoreduction of ferredoxin and its use in carbon dioxide fixation by a subcellular system from a photosynthetic bacterium. Proc Natl Acad Sci USA 53: 1420–1425

    PubMed  Google Scholar 

  • Feiler U and Hauska G (1995) The reaction center from green sulfur bacteria, In: Blankenship RE, Madigan MT and Bauer CE (eds) Anoxygenic Photosynthetic Bacteria, pp 665–685. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Feiler U, Nitschke W and Michel H (1992) Characterization of an improved reaction center preparation from the photosynthetic green sulfur bacterium Chlorobium containing the FeS centers FA and FB and a bound cytochrome subunit. Biochemistry 31: 2608–2614

    Article  PubMed  Google Scholar 

  • Fischer U (1988) Soluble electron-transfer proteins of Chlorobiaceae. In: Olson JM, Ormerod JG, Amesz J, Stackebrandt E and Trueper HG (eds) Green Photosynthetic Bacteria, pp 127–131. Plenum Press, New York/London

    Google Scholar 

  • Fowler CF, Nugent NA and Fuller RC (1971) The isolation and characterization of a photochemically active complex from Chloropseudomonas ethylica. Proc Natl Acad Sci USA 68: 2278–2282

    PubMed  Google Scholar 

  • Franken EM and Amesz J (1997) Electron transport and triplet formation in membrane fragments of the green sulfur bacterium Prosthecochloris aestuarii. Biochim Biophys Acta 1319: 214–222

    Google Scholar 

  • Jenney FE Jr and Daldal F (1993) A novel membrane-associated ctype cytochrome, cyt cy, can mediate the photosynthetic growth of Rhodobacter capsulatus and Rhodobacter sphaeroides. EMBO J 12: 1283–1292

    PubMed  Google Scholar 

  • Jenney FE Jr, Prince RC and Daldal F (1994) Roles of the soluble cytochrome c2 and membrane-associated cytochrome cy of Rhodobacter capsulatus in photosynthetic electron transfer. Biochemistry 33: 2496–2502

    Article  PubMed  Google Scholar 

  • Kjær B and Scheller HV (1996) An isolated reaction center complex from the green sulfur bacterium Chlorobium vibrioforme can photoreduce ferredoxin at high rates. Photosynth Res 47: 33–39

    Article  Google Scholar 

  • Klughammer C, Hager C, Padan E, Schütz M, Schreiber U, Shahak Y and Hauska G (1995) Reduction of cytochromes with menaquinol and sulfide in membranes from green sulfur bacteria. Photosynth Res 43: 27–34

    Article  Google Scholar 

  • Kusai K and Yamanaka T (1973) The oxidation mechanisms of thiosulphate and sulphide in Chlorobium thiosulphatophilum: roles of cytochrome c-551 and cytochrome c-553. Biochim Biophys Acta 325: 304–314

    PubMed  Google Scholar 

  • Kusumoto N, Inoue K, Nasu H and Sakurai H (1994) Preparation of a photoactive reaction center complex containing photoreducible Fe-S centers and photooxidizable cytochrome c from the green sulfur bacterium Chlorobium tepidum. Plant Cell Physiol 35: 17–25

    Google Scholar 

  • Kusumoto N, Inoue K and Sakurai H (1995) Spectroscopic studies of bound cytochrome c and an iron-sulfur center in a puri-fied reaction center complex from the green sulfur bacterium Chlorobium tepidum. Photosynth Res 43: 107–112

    Article  Google Scholar 

  • Kusumoto N, Sétif P, Brettel K, Seo D and Sakurai H (1999) Electron transfer kinetics in purified reaction centers from the green sulfur bacterium Chlorobium tepidum studied by multiple-flash excitation. Biochemistry 38: 12124–12137

    Article  PubMed  Google Scholar 

  • Malkin M (1987) Photosystem I. In: Barber J (ed) The Light Reactions, pp 495–525. Elsevier Science Publishers, Amsterdam/New York/Oxford

    Google Scholar 

  • Matsuura K and Shimada K (1990) Evolutionary relationships between reaction center complexes with and without cytochrome c subunits in purple bacteria. In: Baltscheffsky M (ed) Current Research in Photosynthesis, Vol I, pp 193–196. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Meyer TE, Bartsch RG, Cusanovich MA and Mathewson JH (1968) The cytochromes of Chlorobium thiosulfatophilum. Biochim Biophys Acta 153: 854–861

    PubMed  Google Scholar 

  • Meyer TE and Donohue TJ (1995) Cytochromes, iron-sulfur, and copper proteins mediating electron transfer from cyt bc1 complex to photosynthetic reaction center complexes. In: Blankenship RE, Madigan MT and Bauer CE (eds) Anoxygenic Photosynthetic Bacteria, pp 725–745. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Oh-oka H, Kamei S, Matsubara H, Iwaki M and Itoh S (1995) Two molecules of cytochrome c function as the electron donors to P840 in the reaction center complex isolated from a green sulfur bacterium Chlorobium tepidum. FEBS Lett 365: 30–34

    Article  PubMed  Google Scholar 

  • Oh-oka H, Iwaki M and Itoh S (1997) Viscosity dependence of the electron transfer rate from bound cytochrome c to P840 in the photosynthetic reaction center of the green sulfur bacterium Chlorobium tepidum. Biochemistry 36: 9267–9272

    Article  PubMed  Google Scholar 

  • Oh-oka H, IwakiMand Itoh S (1998) Membrane-bound cytochrome cz couples quinol oxidoreductase to the P840 reaction center complex in isolated membranes of the green sulfur bacterium Chlorobium tepidum. Biochemistry 37: 12293–12300

    Article  PubMed  Google Scholar 

  • Okkels JS, Kjær B, Hansson O, Svendsen I, Møller BL and Scheller HV (1992) A membrane-bound monoheme cytochrome c551 of a novel type is the immediate electron donor to P840 of the Chlorobium vibrioforme photosynthetic reaction center complex. J Biol Chem 267: 21139–21145

    PubMed  Google Scholar 

  • Okumura N, Shimada K and Matsuura K (1994) Photo-oxidation of membrane-bound and soluble cytochrome c in the green sulfur bacterium Chlorobium tepidum. Photosynth Res 41: 125–134

    Article  Google Scholar 

  • Olson JM, Prince RC and Brune DC (1976) Reaction-center complexes from green bacteria. Brookhaven Symp Biol 28: 238–246

    PubMed  Google Scholar 

  • Ortega JM, Drepper F and Mathis P (1999) Electron transfer between cytochorme c2 and the tetraheme cytochrome c in Rhodopseudomonas viridis. Photosynth Res 59: 147–157

    Article  Google Scholar 

  • Overfield RE and Wraight CA (1980) Oxidation of cytochromes c and c2 by bacterial photosynthetic reaction centers in phospholipid vesicles. 1. Studies with neutral membranes. Biochemistry 19: 3322–3327

    Article  PubMed  Google Scholar 

  • Prince RC and Olson JM (1976) Some thermodynamic and kinetic properties of the primary photochemical reactants in a complex from a green photosynthetic bacterium. Biochim Biophys Acta 423: 357–362

    PubMed  Google Scholar 

  • Sakurai H, Kusumoto N and Inoue K (1996) Function of the reaction center of green sulfur bacteria. Photochem Photobiol 64: 5–13

    Google Scholar 

  • Schmidt KA, Neerken S, Permentier HP, Hager-Braun C and Amesz J (2000) Electron transfer in reaction center core complexes from the green sulfur bacteria Prosthecochloris aestuarii and Chlorobium tepidum. Biochemistry 39: 7212–7220

    Article  PubMed  Google Scholar 

  • Selvaraj F, Devine D, Zhou W, Brune DC, Lince MT and Blankenship RE (1998) Purification and properties of cytochrome c-553 from the green sulfur bacterium Chlorobium tepidum. In: Garab G (ed) Photosynthesis: Mechanisms and Effects, Vol III, pp 1593–1596. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Seo D, Tomioka A, Kusumoto N, Kamo M, Enami I and Sakurai H (2001) Purification of ferredoxins and their reaction with purified reaction center complex from the green sulfur bacterium Chlorobium tepidum. Biochim Biophys Acta 1503: 377–384

    PubMed  Google Scholar 

  • Sétif P, Seo D and Sakurai H (2001) Photoreduction and reoxidation of the three iron-sulfur clusters of reaction centers of green sulfur bacteria. Biophys J 81: 1208–1219

    PubMed  Google Scholar 

  • Steinmetz MA and Fischer U (1982) Cytochromes of the green sulfur bacterium Chlorobium vibrioforme f. thiosulfatophilum. Puri-fication, characterization and sulfur metabolism. Arch Microbiol 131: 19–26

    Article  Google Scholar 

  • Yamanaka T (1992) The Biochemistry of Bacterial Cytochromes. Japan Scientific Societies Press, Tokyo/Springer-Verlag, Berlin

    Google Scholar 

  • Yamanaka T and Okunuki K (1968) Comparison of Chlorobium thiosulphatophilum cytochrome c-555 with c-type cytochromes derived from algae and nonsulphur purple bacteria. J Biochem 63: 340–346

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, School of Education and Division of Bioscience & Bioengineering, Graduate School of Science and Engineering, Waseda University, Nishiwaseda 1, Shinjuku, Tokyo, 169-8050, Japan

    Masaaki Itoh, Daisuke Seo & Hidehiro Sakurai

  2. DBCM/SBE and URA CNRS 2096, CEA Saclay, Gif sur Yvette, 91191, France

    Pierre Sétif

Authors
  1. Masaaki Itoh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Daisuke Seo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hidehiro Sakurai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pierre Sétif
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hidehiro Sakurai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Itoh, M., Seo, D., Sakurai, H. et al. Kinetics of electron transfer between soluble cytochrome c-554 and purified reaction center complex from the green sulfur bacterium Chlorobium tepidum . Photosynthesis Research 71, 125–135 (2002). https://doi.org/10.1023/A:1014959715666

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1014959715666

Search

Navigation