Abstract
In this work, studies on the phenomenon of concealed heterokaryosis that we previously detected in the Saccharomycetes yeast strains were continued. New approaches to high effectiveness of isolation of cytoductants carrying the concealed nucleus were implemented, and the composition of individual concealed heterokaryons, zygotic clones, and the first zygotic buds was analyzed by a micromanipulation technique. The relationship between a delay in the growth of the parental strain (a potential donor of the concealed nucleus) and a decline in the frequency of the appearance of concealed heterokaryons (HKC) was observed. It is assumed that different replication rates of two nuclei of the heterokaryon probably underlie the appearance of HKC. A drastically decreased level of replication of one of the parental nuclei may be connected with the fact that binuclear buds appear extremely rarely and give rise to the rapidly “purified” progeny consisting of cells carrying the second nucleus with normal replication. A lack of the phenotype allows rare binuclear cells to persist as concealed heterokaryons. HKC may be detected only when cells of either parental type are isolated on the corresponding selective media.
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REFERENCES
Nevzglyadova, O.V., Davydenko, S.G., Smirnova, T.I., and Soidla, T.R., Cryptic Heterokaryons in Saccharomy-ces cerevisiae Strains: Pseudopleiotropic Suppression in Strain YPH857 Carrying Multiple Markers, Genetika (Moscow), 1998, vol. 34, no. 12, pp. 1597-1602.
Nevzglyadova, O.V., Davydenko, S.G., Smirnova, T.I., and Soidla, T.R., Cryptic Heterokaryons in Saccharomy-ces cerevisiae Strains: Model Experiments, Genetika (Moscow), 1998, vol. 34, no. 12, pp. 1603-1609.
Nevzglyadova, O.V., Smirnova, T.I., Gaivoronskii, A.A., and Soidla, T.R., Cryptic Heterokaryons in Saccharomy-ces cerevisiae Strains: Prerequisites to the Manifestation of a Cryptic Nucleus, Genetika (Moscow), 2000, vol. 36, no. 8, p. 1017.
Nevzglyadova, O.V., Gaivoronskii, A.A., Artemov, A.V., et al., Detection of Cryptic “Illegitimate” Nuclei in Tet-rad Analysis of the Diploid Progeny of Heterokaryons in Saccharomyces cerevisiae, Genetika (Moscow), 2001, vol. 37, no. 6, pp. 754-761.
Sherman, F., Fink, G.R., and Hicks, J.B., Laboratory Course Manual for Methods in Yeast Genetics, Cold Spring Harbor, New York: Cold Spring Harbor Lab., 1986.
Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Lab., 1989.
Gebeyehu, G., Rao, P.Y., and Chan, P.S., Novel Biotinylated Nucleotide Analogs for Labeling and Colorimetric Detection of DNA, Nucleic Acids Res., 1987, vol. 15, no. 11, pp. 4513-4534.
Sikorski, R.S. and Hieter, P., A System of Shuttle Vectors and Yeast Host Strains Designed for Efficient Manipulation of DNA in Saccharomyces cerevisiae, Genetics, 1989, vol. 122, pp. 19-27.
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Nevzglyadova, O.V., Gaivoronski, A.A., Artyomov, A.V. et al. Factors Affecting the Frequency of Concealed Heterokaryosis in Saccharomyces cerevisiae. Russian Journal of Genetics 38, 223–229 (2002). https://doi.org/10.1023/A:1014856130649
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DOI: https://doi.org/10.1023/A:1014856130649