Abstract
Two physiologically active oligosaccharide fractions were isolated from pectin of Pisum sativum L. cell wall after its partial acid hydrolysis. These fractions displayed stimulating and inhibiting effects on root formation in thin-layer explants. The subsequent separation of these fractions by gel permeation and anion-exchange chromatography resulted in fractions with effective concentrations two orders of magnitude lower than the concentrations of the initial fractions. The resulting oligosaccharides displayed their effect on the earliest stage of the rhizogenesis associated with formation of root primordias. The rhizogenesis-inhibiting fraction suppressed cell division by 30-50%. The stimulating fraction mainly contained fragments of xyloglucan and galactan, and the inhibiting fraction contained fragments of xyloglucan, galactan, and arabinan. The polymerization degrees of the stimulating and of the inhibiting oligosaccharides were 10-11 and 5-6, respectively.
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REFERENCES
Aldington, S., and Fry, S. C. (1993) Adv. Botan. Res., 19, 1–101.
Cote, F., and Hahn, M. (1994) Plant Mol. Biol.,26, 1379–1411.
Zabotina, O. A., Gurjanov, O. P., Ibragimova, N. N., Ayupova, D. A., and Lozovaya, V. V. (1998) Plant Sci., 135, 195–201.
Zabotina, O. A., Gurjanov, O. P., Ayupova, D. A., Beldman, G., Voragen, A. G. J., and Lozovaya, V. V. (1996) Plant Cell Rep., 15, 954–957.
Zabotina, O., Ayupova, D., Larskaya, I., Nikolaeva, O., Petrovicheva, G., and Zabotin, A. (1998) Fiziol. Rast., 45, 262–267.
Aldington, S., McDougall, G. J., and Fry, S. C. (1991) Plant Cell Environ., 14, 625–636.
Liskova, D., Auxtova, O., Kakoniova, D., Kubackova, M., Karacsonyi, S., and Bilics, L. (1995) Planta, 196, 425–429.
Bellincampi, D., Cardarelli, M., Zaghi, D., Serino, G., Salvi, G., Gatz, C., Cervone, F., Altamura, M., Costantino, P., and de Lorenzo, G. (1996) Plant Cell, 8, 477–487.
Tran Thanh Van, K., Toubart, P., Cousson, A., Darvill, A. G., Gollin, D. J., Chelf, P., and Albersheim, P. (1985) Nature, 314, 615–617.
Zabotina, O. A., Gur'yanov, O. P., Malikhov, R. G., Ayupova, D. A., Beldman, G., Voragen, A. J. G., and Lozovaya, V. V. (1995) Fiziol. Rast., 42, 416–422.
Zabotina, O. A., Gur'yanov, O. P., Ayupova, D. A., Lozovaya, V. V., Beldman, G., and Voragen, A. (1997) Biochemistry (Moscow), 62, 845–849.
Zabotina, O., Ibragimova, N., Ayupova, D., Gurjanov, O., Lozovaya, V., Beldman, G., and Voragen, A. (1996) in Pectin and Pectinases (Visser, J., and Voragen, A., eds.) Elsevier Science, Ireland, pp. 693–701.
Ahmed, A. E., and Labavitch, J. M. (1977) J. Food Biochem., 1, 361–365.
Englyst, H. N., and Cummings, J. H. (1984) Analyst, 109, 939–942.
Sandford, P. A., and Conrad, H. E. (1966) J. Biochem., 5, 1508–1517.
Reimers, F. E., and Illi, I. E. (1978) Seed Germination and Temperature [in Russian], Nauka, Novosibirsk, p.104.
Stekol'shchikov, M. G., and Umylina, T. M. (1981) Methodical Manual for Summer Practice in Genetics [in Russian], Kazan State University, Kazan, p. 55.
Turkov, V. D., Guzhov, Yu. L., and Shelepina, G. A. (1988) Studies on Plant Chromosomes in Problems of Selection, Cell Engineering, and Genetic Monitoring [in Russian], People Friendship University, Moscow, p. 63.
Pausheva, Z. P. (1988) Practical Work in Plant Cytology [in Russian], Kolos, Moscow, p. 27.
Wightman, F., and Thimann, K. V. (1980) Physiol. Plant., 49, 13–20.
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Zabotina, O.A., Ibragimova, N.N., Zabotin, A.I. et al. Biologically Active Oligosaccharides from Pectins of Pisum sativum L. Seedlings Affecting Root Generation. Biochemistry (Moscow) 67, 227–232 (2002). https://doi.org/10.1023/A:1014470215762
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DOI: https://doi.org/10.1023/A:1014470215762