Abstract
Experiments were conducted on14C-sorbitol, fructose, and glucose uptakeinto flesh discs, and sorbitol efflux from thediscs, with and without ABA application toexamine the effect of abscisic acid (ABA) onsugar accumulation in peach fruit flesh at thestart of the maturation stage in relation tomembrane transport. Total uptake of14C-sorbitol, fructose, and glucose intoflesh discs was effectively promoted by ABA ata concentration of 10−5 M. PCMBS(p-chloromercuribenzensulfonicacid)-sensitive uptake, which was considered ascarrier-mediated uptake, of sorbitol into thediscs was clearly stimulated by ABA at10−5 M, compared with glucose andfructose uptake. Sorbitol efflux from the discsacross the tonoplast was restricted by ABA at10−5 M. ABA application todeveloping fruit increased sugar accumulationin the fruit. Estimated ABA concentration inthis fruit was approximately 10−5 M. These results indicate that sugar accumulationin peach fruit flesh is stimulated by ABA at aconcentration of 10−5 M both invitro and in vivo. ABA stimulatesuptake of sugars, especially sorbitol, into theflesh by enhancing carrier-mediated transportpossibly across both tonoplast and plasmamembrane.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barkla BJ (1999) Abscisic acid induction of vacuolar H+-ATPase activity in Mesembryanthemun crystallinum is developmentally regulated. Plant Physiol 120: 811–819
Beruter J (1983) Effect of abscisic acid on sorbitol uptake in growing apple fruit. J Exp Bot 34: 737–743
Bourquin S, Bonnemain JL and Delrot S (1990) Inhibition of loading of 14C assimilates by p-chloromerucuribenzensulfonic acid. Plant Physiol 92: 97–102
Bush DR (1993) Proton-coupled sugars and amino acid transporters in plants. Ann Rev Plant Physiol Plant Mol Biol 44: 513–542
Daie J (1985) Carbohydrate partitioning and metabolism in crops. Hort Rev 7: 69–108
Daie J and Wyse R (1983) ABA uptake in source and sink tissues of sugar beet. Plant Physiol 72: 430–433
Delrot S (1989) Loading of photoassimilates. In: Baker DA and Milburn JA (eds) Transport of photoassimilates. Essex: Longman Scientific & Technical, pp 167–205
Fillion L, Ageorges A, Picaud S, Coutos-Thevenot P, Lemoine R, Romieu C and Delrot S (1999) Cloning and expression of a hexose transporter gene expressed during the ripening of grape berry. Plant Physiol 120: 1083–1093
Giaquinta R (1976) Evidence for phloem loading from the apoplast. Plant Physiol 57: 872–875
Ho LC (1988) Metabolism and compartmentation of imported sugars in sink organs in relation to sink strength. Ann Rev Plant Physiol Plant Mol Biol 39: 355–378
Kasai M, Yamamoto Y, Maeshima M and Matsumoto H (1993) Effects of in vivo treatment with abscisic acid and/or cytokinin on activities of vacuolar H+ pumps of tonoplastenriched membrane vesicles prepared from barley roots. Plant Cell Physiol 34: 1107–1115
Kasianowiez J, Benz R and McLaughlin S (1987) The kinetic mechanism by which CCCP (carbonyl cyanide mchlorophenylhydrazone) transports protons across membranes. J Memb Biol 82: 179–190
Kobashi K, Gemma H and Iwahori S (1999) Sugar accumulation in peach fruit as affected by abscisic acid (ABA) treatment in relation to some sugar metabolizing enzymes. J Japan Soc Hort Sci 68: 465–470
Lo Bianco R, Rieger M and Sung SS (1999) Carbohydrate metabolism of vegetative and reproductive sinks in the latematuring peach cultivar 'Encore'. Tree Physiol 19: 103–109
Maynard JW and Lucas WJ (1982) A reanalysis of the twocomponent phloem loading system in Beta vulgaris. Plant Physiol 69: 734–739
Moriguchi T, Sanada T and Yamaki S (1990) Seasonal fluctuations of some enzymes relating to sucrose and sorbitol metabolism in peach fruit. J Amer Soc Hort Sci 115: 278–281
Narasimhan ML, Binzel ML, Perez-Prat E, Chen Z, Nelson DE, Singh NK, Bressan RA and Hasegawa PM (1991) NaCl regulation of tonoplast ATPase 70-kilodalton subunit mRNA in tobacco cells. Plant Physiol 97: 562–568
Ofosu-Anim J and Yamaki S (1994) Sugar content and compartmentation in melon fruit and the restriction of sugar efflux from flesh tissue by ABA. J Japan Soc Hort Sci 63: 685–692
Ofosu-Anim J and Yamaki S (1994) Sugar content, compartmentation and efflux in strawberry tissue. J Amer Soc Hort Sci 119: 1024–1028
Ofosu-Anim J, Kanayama Y and Yamaki S (1996) Sugar uptake into strawberry fruit is stimulated by abscisic acid and indoleacetic acid. Physiol Plant 97: 169–174
Ofosu-Anim J, Kanayama Y and Yamaki S (1998) Changes in sugar uptake by excised discs and its stimulation by abscisic and indoleacetic acid during melon fruit development. J Japan Soc Hort Sci 67: 170–175
Patrick JW(1997) Phloem unloading: sieve element unloading and post-sieve element transport. Ann Rev Plant Physiol Plant Mol Biol 48: 191–222
Uthaibutra J and Gemma H (1991) Changes in abscisic acid content of peel and pulp of Jonagold apples during pre-and post-harvest periods. J Japan Soc Hort Sci 60: 443–448
Vizzotto G, Pinton R, Varanini Z and Costa G (1996) Sucrose accumulation in developing peach fruit. Physiol Plant 96: 225–230
Yamaki S and Asakura T (1988) Energy coupled transport of sorbitol and other sugars into the protoplast isolated from apple fruit flesh. Plant Cell Physiol 29: 961–967
Yamaki S and Asakura T (1991) Stimulation of the uptake of sorbitol into vacuoles from apple fruit flesh by abscisic acid and into protoplast by indoleacetic acid. Plant Cell Physiol 32: 315–318
Yamaki S and Ino M (1992) Alteration of cellular compartmentation and membrane permeability to sugar in immature and mature apple fruit. J Amer Soc Hort Sci 117: 951–954
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kobashi, K., Sugaya, S., Gemma, H. et al. Effect of abscisic acid (ABA) on sugar accumulation in the flesh tissue of peach fruit at the start of the maturation stage. Plant Growth Regulation 35, 215–223 (2001). https://doi.org/10.1023/A:1014421712254
Issue Date:
DOI: https://doi.org/10.1023/A:1014421712254