Abstract
The gene coding for the secreted Serratia marcescens endonuclease was fused with the mannopine synthase promoter of Agrobacterium tumefaciens Ti plasmid and transferred to Nicotiana tabacum SR1 plants. The promoter is leaf- and root-specific. The resulting transgenic plants demonstrated elevated nuclease activity. The level of the transgene product was determined in the transgenic lines.
Similar content being viewed by others
REFERENCES
Naito, K., Iida, A., Suzuki, H., and Tsuji, H., The Effect of Benzyladenine on Changes in Nuclease and Protease Activities in Intact Bean Leaves during Aging, Physiol. Plant., 1979, vol. 46, pp. 50-53.
Plotnikov, V.K., The mRNA Stability as a Factor Regu lating Gene Expression in Eukaryotic Cells, Usp. Sovrem. Biol., 1992, vol. 112, pp. 186-197.
Blank, A. and McKeon, T.A., Single-Strand-Preferring Nuclease Activity in Wheat Leaves Is Increased in Senescence and Is Negatively Photoregulated, Proc. Natl Acad. Sci. USA, 1989, vol. 86, pp. 3169-3173.
Bariola, P.A., Howard, C.J., Taylor, C.B., et al., The Arabidopsis Ribonuclease Gene RNS1 Is Tightly Controlled in Response to Phosphate Limitation, Plant J., 1994, vol. 6, pp. 673-685.
Galiana, E., Bonnet, P., Conrod, S., et al., RNase Activity Prevents the Growth of a Fungal Pathogen in Tobacco Leaves and Increases upon Induction of Systemic Acquired Resistance with Elicitin, Plant Physiol., 1997, vol. 115, pp. 1557-1567.
Abel, P.P., Nelson, R.S., De, B., et al., Delay of Disease Development in Transgenic Plant That Expresses the Tobacco Mosaic Virus Coat Protein Gene, Science, 1986, vol. 232, pp. 738-743.
Wilson, T.M.A., Strategies to Protect Crop Plants against Viruses: Pathogen-Derived Resistance Blossoms, Proc. Natl Acad. Sci. USA, 1993, vol. 90, pp. 3134-3141.
Lindbo, J.A. and Dougherty, W.G., Untranslatable Transcripts of the Tobacco Etch Virus Coat Protein Gene Sequence Can Interfere with Tobacco Etch Virus Replication in Transgenic Plants and Protoplasts, Virology, 1992, vol. 189, pp. 725-733.
Salganic, R.I., Antiviral Activity of Nucleases: Molecular Mechanisms and Applications, Curr. Trends Life Sci. Biol. Macromol., 1984, vol. 12, pp. 115-123.
Wolf, J.Dr., Klinger, P4016483.7, 1991.
Sano, T., Nagayama, A., Ogawa, T., et al., Transgenic Potato Expressing a Double-Stranded RNA-Specific Is Resistant to Potato Spindle Tuber Viroid, Nat. Biotechnol., 1997, vol. 15, pp. 1290-1294.
Rivkin, M.I., Deineko, E.V., Komarova, M.L., et al., Estimation of Virus Resistance in Tobacco and Alfalfa Transgenic Plants Carrying the Human ?-Interferon Gene, Dokl. Ross. Akad. Nauk, 1993, vol. 331, pp. 652-654.
Ball, T.K., Saurugger, P.N., and Benedik, M.J., The Extracellular Nuclease Gene of Serratia marcescens and Its Secretion from Escherichia coli, Gene, 1987, vol. 57, pp. 183-192.
Alliotte, T., Zhu, L.H., Van Montagu, M., and Inze, D., Plant Expression Vectors with the Origin of Replication of the W-Type Plasmid Sa, Plasmid, 1988, vol. 19, pp. 251-254.
Carcana, A., Confalone, E., Libonati, M., et al., Structure of the Bovine Pancreatic Gene: The Unique Intervening Sequence in 5'-Untranslated Regions Contains a Promoter-like Element, Nucleic Acids Res., 1988, vol. 16, pp. 5491-5502.
Sijmons, P.C., Dekker, B.M., Schrammeijer, B., et al., Production of a Correctly Processed Human Albumin in Transgenic Plants, Biotechnology, 1990, vol. 8, pp. 217-221.
Deblare, R., Reynaerts, A., Hofte, H., et al., Vectors for Cloning in Plant Cells, Methods Enzymol., 1987, vol. 153, pp. 277-292.
Gynheung, A.G., Binary Ti Vectors for Plant Transformation and Promoter Analysis, Methods Enzymol., 1987, vol. 153, pp. 292-305.
Reiss, B., Sprendel, R., Will, H., et al., A New Sensitive Method for Qualitative and Quantitative Assay of Neomycin Phosphotransferase in Crude Cell Extracts, Gene, 1984, vol. 30, pp. 211-218.
Bradford, M.M., A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein–Dye Binding, Anal. Biochem., 1976, vol. 72, pp. 248-254.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Trifonova, E.A., Komarova, M.L., Syrnik, O.A. et al. Transgenic Tobacco (Nicotiana tabacum SR1) Plants Expressing the Gene Coding for Serratia marcescens Nuclease. Russian Journal of Genetics 38, 210–212 (2002). https://doi.org/10.1023/A:1014346413868
Issue Date:
DOI: https://doi.org/10.1023/A:1014346413868