Abstract
Activated charcoal, used for decolorization and purification of crude protease, was regenerated by treatment six times with acetone/water (40:60 v/v), followed by drying. This multistage leaching followed the leaching equation adapted to multistage leaching. The regenerated charcoal was nearly as effective as fresh charcoal in decolorization and purification of crude protease, but only after drying.
List of symbols: c *, equilibrium solute concentration (w/v) in liquid; c s, solute concentration in solid phase (w/w); F, interfacial area between solid and liquid phases; k, rate constant for leaching equation; v, volume of liquid per unit weight of adsorbent (solid phase).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aikat K,Bhattacharyya BC (2000) Protease extraction in solid state fermentation of wheat bran by a local strain of Rhizopus oryzae and growth studies by the soft gel technique. Proc. Biochem. 35: 907-914.
Aikat K,Maiti TK,Bhattacharyya BC (2001) Decolorization and purification of crude protease from Rhizopus oryzae by activated charcoal and its electrophoretic analysis. Biotechnol. Lett. 23: 295-301.
Banerjee R.,Bhattacharyya BC (1992) Extracellular alkaline protease of newly isolated Rhizopus oryzae. Biotechnol. Lett. 14: 301-304.
Gutcho SJ (1974) Microbial Enzyme Production. USA: Noyes Data Corporation, pp. 230-237.
Hassler JW (1951) Active Carbon. New York: Chemical Publishing Co., Inc.
Hassler JW (1974) Purification with Activated Carbon: Industrial, Commercial, Environmental. New York: Chemical Publishing Co., Inc.
Liley PE,Reid RC,Buck E (1984) Physical and chemical data. In: Perry RH,Green DW,Maloney JO, eds. Perry' Chemical Engineers’ Handbook, International edn. Singapore: McGraw-Hill Book Co., pp. 3251-3252.
Maggs FAP (1968) Activated charcoal (active carbon). In: Davidson HW,Wiggs PKC,Churchhouse AH,Maggs FAP,Bradley RS, eds. Manufactured Carbon. London: Pergamon Press, pp. 81-101.
Okuyama N,Seta K,Ishikawa T (1995) Device for protein removal. Jpn. Kokai Tokkyo Koho JP 07,300,499 [95,300,499] (Cl.C07 K14/765), 14 Nov 1995 [as cited in Chemical Abstracts No. 169987, 124(13), 1996].
Patil M,Shastri NV (1981) Extracellular proteases by Alternaria alternata (Fr.) Keissl. J. Ferment. Technol. 59: 403-406.
Pradhan BK,Sandle NK (1999) Effect of different oxidizing agent treatments on the surface properties of activated carbons. Carbon 37: 1323-1332.
Rodwell VW (1988) Proteins: structure & properties. In: Murray RK,Granner DK,Mayes PA,Rodwell VW, eds. Harper' Biochemistry, 21st edn. Connecticut: Appleton and Lange, pp. 32-39.
Schaumberg JP,Hokanson GC,French JC,Smal E,Baker DC (1985) 2-Chloropentostatin, a new inhibitor of adenosinedeaminase. J. Org. Chem. 50: 1651-1656.
Stevens RE,Billingsley KC (1998) Production, extraction, and qualitative testing of penicillin: a biochemistry experiment for health science chemistry courses. J. Chem. Educ. 75: 1264-1265.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Aikat, K., Bhattacharyya, B.C. Regeneration of activated charcoal used in decolorization and purification of crude protease from Rhizopus oryzae . Biotechnology Letters 23, 1915–1919 (2001). https://doi.org/10.1023/A:1013705600716
Issue Date:
DOI: https://doi.org/10.1023/A:1013705600716