Abstract
Stem apical meristems, rhizome apical meristems and rhizome axillary meristems excised from Alstroemeria plants were grown in vitro on modified Murashige and Skoog (MS) media containing different concentrations of gibberellic acid and 6-benzylaminopurine (BA). Plantlets developed from stem apical meristems never regenerated a rhizome and eventually died. The highest regeneration rate (74.1%) of plantlets with a rhizome was observed when rhizome axillary meristems were grown on modified MS medium containing μM 8.9 of BA. Alstroemeria mosaic potyvirus (AlMV) could be eradicated from infected Alstroemeria plants through meristem culture. The rate of virus eradication was 73.7 and 14.7% for plantlets developing from explants measuring 0.7 mm and 2.0 mm, respectively. Greenhouse evaluation of virus-negative and AlMV-infected Alstroemeria plants showed that healthy plants produced more floral stems, more vegetative stems, longer floral stems and gave a higher fresh weight than infected plants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brunt AA & Phillips S (1981) Alstroemeria. Rep. Glasshouse Crops Res. Inst. for 1979 (p 151)
Hakkaart FA & Versluijs JMA (1985) Viruses of Alstroemeria and preliminary results of meristem tip culture. Acta Hort. 164: 71–75
Hakkaart FA & Versluijs JMA (1988) Virus elimination by meristem-tip culture from a range of Alstroemeria cultivars. Neth. J. Pl. Path. 94: 49–56
Lilien-Kipnis H, Stein A & Levy S (1992) The course of bean yel-low mosaic virus elimination from Gladiolus by meristem tip culture. Acta Hort. 325: 709–714
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497
Phillips S & Brunt AA (1983) Alstroemeria. Rep. Glasshouse Crops Res. Inst. for 1981 (142 p)
Phillips S & Brunt AA (1986) Four viruses of Alstroemeria in Britain. Acta Hort. 177: 227–233
SAS Institute Inc. 1989. SAS/STAT user's guide, version 6, Fourth edition. SAS Institute Inc., Cary, NC, USA
Sutula CL, Gillett JM, Morrissey SM & Ramsdell DC (1986) Interpreting ELISA data and establishing the positive–negative threshold. Plant Disease 70(8): 722–726
Van Zaayen A (1995) Alstroemeria. In: Loebenstein G, Lawson RH & Brunt AA (eds) Virus and Virus-like Diseases of Bulbs and Flower Crops, (pp. 237–249). Wiley Publishers, Chichester, West Sussex, UK
Van Zaayen A, Van Eijk C & Versluijs JMA (1992). Production of healthy ornamental crops through meristem culture. Acta Bot. Neerl. 41: 425–433
Wong SM, Chng CG, Reiser RA & Horst RK (1994) Further char-acterization of a potyvirus in Alstroemeria Endowment Series. Acta Hortic. 377: 63–71
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chiari, A., Bridgen, M.P. Meristem culture and virus eradication in Alstroemeria. Plant Cell, Tissue and Organ Culture 68, 49–55 (2002). https://doi.org/10.1023/A:1012947401640
Issue Date:
DOI: https://doi.org/10.1023/A:1012947401640