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Micropropagation of Corydalis ambigua through embryogenesis of tuber sections and chemical evaluation of the ramets

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Abstract

Corydalis ambigua, a perennial herb of the family Papaveraceae, was micropropagated through somatic embryogenesis starting from sliced tubers. Somatic embryos were proliferated on Linsmaier and Skoog medium of a half strength containing 2% sucrose and 0.1 μM indole-3-acetic acid or indole-3-butyric acid solidified with 0.2% Gelrite. Somatic embryos were germinated and grew on plant growth regulator-free White's medium supplemented with 2% sucrose and 0.8% agar in the dark at 0–4°C for 6 months to give rise to microtubers that could be potted out of culture tubes. Three strains of micropropagated plants cultivated outdoors for 5 years gave different tetrahydroprotoberberine-type alkaloids pattern, respectively. The variation of tetrahydroprotoberberine-type alkaloid (corybulbine, corydaline and cavidine) content within a strain was not significantly different from that of the corresponding alkaloid in the wild plants.

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References

  • Chou YH & Hsü CC (1992) Corydalis turtschaninovii Gess. f. yanhusuo. In: Tang W & Eisenbrand G (ed) Chinese Drugs of Plant Origin (pp 377–393). Springer, Berlin

    Google Scholar 

  • Diettrich B, Schneider V & Luckner M (1991) High variation in cardenolide content of plants regenerated from protoplasts of the embyrogenic cell strain VII of Digitalis lanata. J. Plant Physiol. 139: 199–204

    Google Scholar 

  • George EF (1993) Plant Propagation by Tissue Culture. Exegetics Ltd., Edington

    Google Scholar 

  • Hiraoka N, Kodama T, Oyanagi M, Nakano S & Satake M (1986) Characteristics of Bupleurum falcatum plants propagated through somatic embryogenesis of callus. Plant Cell Rep. 5: 319–321

    Google Scholar 

  • Hotta M, Ogata K, Hosikawa K, Yanagi M & Yamazaki K (1989) Useful Plants of the World. Heibonsha, Tokyo

    Google Scholar 

  • Linsmaier EM & Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100–127

    Google Scholar 

  • Miura Y, Fukui H & Tabata M (1988) Reduced inhomogeneity of Angelica actutiloba plants propagated clonally through somatic embryoids. Planta Med. 54: 79–81

    Google Scholar 

  • Ohara M & Higashi S (1994) Effects of inflorescence size on visits from pollinators and seed set of Corydalis ambigua (Papaveraceae). Oecologia 98: 25–30

    Google Scholar 

  • Pal A & Roy A (1991) Embryo culture of Costus speciosus (Koen.) Sm. to regenerate variable diosgenin yielding clones. Plant Cell Rep. 10: 565–568

    Google Scholar 

  • Yasaka M, Nishiwaki Y & Konno Y (1998) Plasticity of flower longevity in Corydalis ambigua. Ecol. Res. 13: 211–216

    Google Scholar 

  • Yasuda M, Kano Y & Konoshima M (1985) Pharmacognostical and botanical studies on the crude drugs in Hokkaido (Part III) Cultivation of Corydalis ambigua Cham. et Schlecht. (I) Germination and first age plant. Shoyakugaku Zasshi 39: 208–213

    Google Scholar 

  • Yasuda M, Konoshima M & Kano Y (1986) Pharmacognostical and botanical studies on the crude drugs in Hokkaido (Part IV) Determination of tertiary alkaloids in Corydalis ambigua by high performance liquid chromatography. Shoyakugaku Zasshi 40: 61–64

    Google Scholar 

  • Yasuda M, Kano Y & Konoshima M (1987a) Pharmacognostical and botanical studies on the crude drugs in Hokkaido (Part VI) Cultivation of Corydalis ambigua Cham. et Schlecht. (II) Germination (supplement) and plant renewal. Shoyakugaku Zasshi 41: 200–204

    Google Scholar 

  • Yasuda M, Konoshima M & Kano Y (1987b) Pharmacognostical and botanical studies on the crude drugs in Hokkaido (Part V) Relation between corydaline and dehydrocorydaline (DHC) in Corydalis ambigua tubers. Shoyakugaku Zasshi 41: 195–199

    Google Scholar 

  • Yasuda M, K ano Y & Konoshima M (1988a) Pharmacognostical and botanical studies on the crude drugs in Hokkaido (Part VII) Cultivation of Corydalis ambigua Cham. et Schlecht. (III) Seasonal growth of tuners and their alkaloid content. Shoyakugaku Za sshi 42: 122–124

    Google Scholar 

  • Yasuda M, Kano Y & Konoshima M (1988b) Pharmacognostical and botanical studies on the crude drugs in Hokkaido (Part VIII) Cultivation of Corydalis ambigua Cham. et Schlecht. (IV) From 3rd age plant to 6th age plant. Shoyakugaku Zasshi 42: 208–213

    Google Scholar 

  • Yasuda M, Kano Y & Konoshima M (1988c) Pharmacognostical and botanical studies on the crude drugs in Hokkaido (Part IX) Cultivation of Corydalis ambigua Cham. et Schlecht. (V) Annual variation of alkaloid contents. Shoyakugaku Zasshi 42: 214–215

    Google Scholar 

  • Zhao L, Yan X & Zhang Z (1988) Tuber formation in tissue culture of Corydalis yanhusuo W. T.Wang. Zhongyao Tongbao 13: 595–596

    Google Scholar 

  • Zilis MR & Zwagerman D (1980) In vitro characteristics of nucellar and adventitious embryoids in the genus Hosta. HortScience 15: 3

    Google Scholar 

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Authors and Affiliations

  1. Niigata College of Pharmacy, 5-13-2 Kamishinei-cho, Niigata, 950-2081, Japan

    Noboru Hiraoka, Yumiko Kato, Yukiko Kawaguchi & Jung I. Chang

Authors
  1. Noboru Hiraoka
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  2. Yumiko Kato
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  3. Yukiko Kawaguchi
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  4. Jung I. Chang
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Correspondence to Noboru Hiraoka.

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Hiraoka, N., Kato, Y., Kawaguchi, Y. et al. Micropropagation of Corydalis ambigua through embryogenesis of tuber sections and chemical evaluation of the ramets. Plant Cell, Tissue and Organ Culture 67, 243–249 (2001). https://doi.org/10.1023/A:1012739624062

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  • DOI: https://doi.org/10.1023/A:1012739624062

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