biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:321-324, 2001 | DOI: 10.1023/A:1012415004676

Ex Vitro Phenotype Stability is Affected by In Vitro Cultivation

D. Haisel1, P. Hofman1, M. Vágner1, H. Lipavská2, I. Tichá2, C. Schäfer3, V. Čapková1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 6, Czech Republic
2 Department of Plant Physiology, Faculty of Science, Charles University, Praha 2, Czech Republic
3 Lehrstuhl für Pflanzenphysiologie, Universität Bayreuth, Bayreuth, Germany

Plant phenotype stability during ex vitro growth, one of the main requirements of plant micropropagation, was tested on tobacco. Plants cultivated in vitro in the presence of 3 % sucrose under photon flux density (PFD) of 200 μmol m-2 s-1 (3 % HL plants) showed the best growth and photosynthetic parameters in the course of 7-day acclimation. However, significant change in phenotype of these plants appeared under a decrease in PFD to 50 μmol m-2 s-1 during further ex vitro growth (in the period of 7th - 17th day). Much higher internodia elongation was found in 3 % HL plants in comparison with plants grown in vitro on sucrose media under PFD of 50 μmol m-2 s-1 (3 % LL) or without sucrose either under PFD of 50 μmol m-2 s-1 or 200 μmol m-2 s-1 (0 % LL, 0 % HL). It can be presumed that 3 % HL plants show permanent demand for high PFD. Neither ABA or chlorophyll contents nor de novo thylakoid membrane synthesis were related to the morphogenic effect of low PFD. Changeable contents of hexoses in leaves of 3 % HL and 3 % LL plants were in no direct correlation to the elongated growth.

Keywords: ABA; chlorophyll; D1 protein synthesis; Nicotiana tabacum; sugars; tobacco
Subjects: abscisic acid; chlorophylls, phenotype stability ex vitro after in vitro culture; D1 protein synthesis, phenotype stability ex vitro after in vitro culture; ex vitro phenotype stability after culture in vitro; in vitro culture, phenotype stability ex vitro; in vitro culture, somatic embryogenesis; in vitro culture, sugars as C source, low sugar media; irradiance, phenotype stability ex vitro after in vitro culture; Nicotiana tabacum; phenotype stability ex vitro after in vitro culture; photoautotrophy, in vitro culture; photomixotrophy, in vitro culture; somatic embryogenesis; sugars, sucrose as carbon source, in vitro culture; tobacco

Published: September 1, 2001  Show citation

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Haisel, D., Hofman, P., Vágner, M., Lipavská, H., Tichá, I., Schäfer, C., & Čapková, V. (2001). Ex Vitro Phenotype Stability is Affected by In Vitro Cultivation. Biologia plantarum, 44(3), 321-324. doi: 10.1023/A:1012415004676
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