Skip to main content
SpringerLink
Log in

Regulation of Reproductive Function in DrosophilaFemales Due to Hormonal Interaction Under Stress Is Genetically Determined

  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

The effect of heat stress (38°C) on the content of octopamine (OA) and 20-hydroxyecdysone (20HE) was studied under normal and stressful conditions in adult flies of Drosophila virilislines contrasting in the level of the juvenile hormone (JH). The wild-type flies (line 101) exhibited a pronounced sex dimorphism for the content of both OA and 20HE, which was substantially lower in this line than in flies of the mutant line 147. The level of both hormones increased in flies of line 101 exposed to heat stress, whereas it remained unchanged in flies of line 147 under the same conditions. The effect of heat stress on the level of JH metabolism and fertility was also studied in D. melanogasterwild-type lines and lines carrying mutations in genes responsible for OA and DA syntheses. In octopamineless females of the Tβh nM18line and in females of the Steline characterized by a doubled content of DA, JH degradation differed from normal: it was increased in both young and mature Tβh nM18females, while decreased in young and increased in mature Steflies. Fertility was substantially lower in the Stethan in the wild-type line. Flies of all of the D. melanogasterlines produced a stress response; however, in mutant lines, both fertility and stress reactivity of the systems controlling JH metabolism differed significantly from that of the wild-type lines. The role of JH, 20HE, OA, and DA interaction in regulation of Drosophilareproduction under stressful conditions is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

REFERENCES

  1. Postlethwait, J.H. and Shirk, P.D., Genetics and Endocrine Regulation of Vitellogenesis in Drosophila, Am. Zool., 1981, vol. 21, pp. 687-700.

    Google Scholar 

  2. Koerre, J.K., Fuchs, M., Chen, T.T., et al., The Role of Juvenile Hormone in Reproduction, in Comprehensive Insect Physiology, Biochemistry and Pharmacology, New York: Pergamon, 1985, vol. 8, pp. 165-203.

    Google Scholar 

  3. Bownes, M., Ronaldson, E., Mauchline, D., and Martinez, A., Regulation of Vitellogenesis in Drosophila, Int. J. Insect Morphol. Embryol., 1993, vol. 22, pp. 349-367.

    Google Scholar 

  4. Jowett, T. and Postlethwait, J., Hormonal Regulation of Synthesis of Yolk Proteins and a Larvae Serum Protein (LSP) in Drosophila, Nature, 1981, vol. 292, pp. 633-635.

    Google Scholar 

  5. An, W. and Wensink, P.C., Integrating Sex-and Tissue-Specific Regulation within a Single Drosophila Enhancer, Genes Dev., 1995, vol. 9, pp. 256-266.

    Google Scholar 

  6. Soller, M., Bownes, M., and Kubli, E., Control of Oocyte Maturation in Sexually Mature Drosophila Females, Dev. Biol., 1999, vol. 208, pp. 337-351.

    Google Scholar 

  7. Redfern, C.P.F. and Bownes, M., Pleiotropic Effects of the ecdysonless-1 Mutation of Drosophila melanogaster, Mol. Gen. Genet., 1983, vol. 189, pp. 432-440.

    Google Scholar 

  8. Bownes, M. and Reid, G., The Role of the Ovary and Nutritional Signals in the Regulation of Fat Body Yolk Protein Gene Expression in Drosophila melanogaster, J. Insect Physiol., 1990, vol. 36, pp. 471-480.

    Google Scholar 

  9. Richard, D.S., Watkins, N.L., Serafin, R.B., and Gilbert, L.I., Ecdysteroids Regulate Yolk Protein Uptake by Drosophila melanoganter Oocytes, J. Insect Physiol., 1998, vol. 44, pp. 637-644.

    Google Scholar 

  10. Thompson, C.S., Yagi, K.J., Chen, Z.F., and Tobe, S.S., The Effects of Octopamine on Juvenile Hormone Biosynthesis, Electrophysiology, and cAMP Content of the Corpora Allata of the Cockroach Diploptera punctata, J. Comp. Physiol. B, 1990, vol. 160, pp. 241-249.

    Google Scholar 

  11. Lafon-Cazal, M. and Baehr, J.C., Octopaminergic Control of Corpora Allata Activity in an Insect, Experentia, 1988, vol. 44, pp. 895-896.

    Google Scholar 

  12. Kaatz, H., Eichmuller, S., and Kreissl, S., Stimulatory Effect of Octopamine on Juvenile Hormone Biosynthesis in Honey Bees (Apis mellifera): Physiological and Immunocytochemical Evidence, J. Insect Physiol., 1994, vol. 40, pp. 856-872.

    Google Scholar 

  13. Rachinsky, A., Octopamine and Serotonin Influence on Corpora Allata Activity in Honey Bee (Apis mellifera) Larvae, J. Insect Physiol., 1994, vol. 40, pp. 549-554.

    Google Scholar 

  14. Pastor, D., Piulachs, M.D., Gassier, P., et al., In Vivo and In Vitro Study of the Action of Dopamine on Oocyte Growth and Juvenile Hormone Production in Blattella germanica, C.R. Acad. Sci. III, 1991, vol. 313, pp. 207-212.

    Google Scholar 

  15. Hirashima, A., Suetsugu, E., Hirokado, S., et al., Effect of Octopamine on the Activity of Juvenile-Hormone Esterase in the Silkworm Bombyx mori and the Red Flour Beetle Tribolium freemani, Gen. Comp. Endocrinol., 1999, vol. 116, pp. 373-381.

    Google Scholar 

  16. Rauschenbach, I.Yu., Lukashina, N.S., and Korochkin, L.I., Genetic of Esterases in Drosophila: VIII. The Gene Controlling the Activity of JH Esterase in D. virilis, Biochem. Genet., 1984, vol. 22, pp. 5-80.

    Google Scholar 

  17. Rauschenbach, I.Yu., Lukashina, N.S., Maksimovsky, L.F., and Korochkin, L.I., Stress-like Reaction of Drosophila to Adverse Environmental Factors, J. Comp. Physiol. B, 1987, vol. 157, pp. 519-531.

    Google Scholar 

  18. Rauschenbach, I.Yu., Serova, L.I., Timochina, I.S., et al., Analysis of Differences in Dopamine Content between Two Lines of Drosophila virilis in Response to Heat Stress, J. Insect Physiol., 1993, vol. 39, pp. 761-767.

    Google Scholar 

  19. Monastirioti, M., Linn, C.E., Jr., and White, K., Characterization of Drosophila Tyramine β-Hydroxylase Gene and Isolation of Mutant Flies Lacking Octopamine, J. Neurosci., 1996, vol. 16, pp. 3900-3911.

    Google Scholar 

  20. Hodgetts, R.B. and Konopka, R.J., Tyrosine and Catecholamine Metabolism in Wild-Type Drosophila melanogaster and a Mutant, ebony, J. Insect Physiol., 1973, vol. 19, pp. 1211-1220.

    Google Scholar 

  21. Walter, M.F., Zeineh, L.L., Black, B.C., et al., Catecholamine Metabolism and In Vitro Induction of Premature Cuticle Melanization in Wild-Type and Pigmentation Mutants of Drosophila melanogaster, Arch. Insect Biochem. Physiol., 1996, vol. 31, pp. 219-233.

    Google Scholar 

  22. Rauschenbach, I.Yu., Gruntenko, N.E., Khlebodarova, T.M., et al., The Role of the Degradation System of the Juvenile Hormone in the Reproduction of Drosophila under Stress, J. Insect Physiol., 1996, vol. 42, pp. 735-742.

    Google Scholar 

  23. Hammock, B.D. and Sparks, T.C., A Rapid Assay for Insect Juvenile Hormone Esterase Activity, Anal. Biochem., 1977, vol. 82, pp. 573-579.

    Google Scholar 

  24. Hirashima, A., Sukhanova, M.Jh., and Rauschenbach, I.Yu., Genetic Control of Biogenic Amine System in Drosophila under Normal and Stress Conditions, Biochem. Genet., 2000, vol. 38, pp. 167-180.

    Google Scholar 

  25. Chang, E.S. and O'Connor, J.D., Arthropod Molting Hormones, Methods of Hormone Radioimmunoassay, Jaffe, B.M. and Behrman, H.R., Eds., New York: Academic, 1979, 2nd ed., pp. 797-814.

    Google Scholar 

  26. Rauschenbach, I.Yu., Khlebodarova, T.M., Chentsova, N.A., et al., Metabolism of the Juvenile Hormone in Drosophila Adults under Normal Conditions and Heat Stress, J. Insect Physiol., 1995, vol. 41, pp. 179-189.

    Google Scholar 

  27. Gruntenko, N.E., Khlebodarova, T.M., Sukhanova, M.Jh., et al., Prolonged Negative Selection of Drosophila melanogaster for a Character of Adaptive Significance Disturbs Stress Reactivity, Insect Biochem. Mol. Biol., 1999, vol. 29, pp. 445-452.

    Google Scholar 

  28. Gruntenko, N.E., Monastirioti, M., Wilson, T.G., and Rauschenbach, I.Yu., Stress Reactivity and Juvenile Hormone Degradation in Drosophila melanogaster Strains Having Stress-Related Mutations, Insect Biochem. Mol. Biol., 2000, vol. 30, pp. 775-783.

    Google Scholar 

  29. Altaratz, M., Applebaum, Sh.W., Richard, D.S., et al., Regulation of Juvenile Hormone Synthesis in Wild-Type and Apterous Mutant Drosophila, Mol. Cell. Endocrinol., 1991, vol. 81, pp. 205-216.

    Google Scholar 

  30. Hammock, B.D., Regulation of Juvenile Hormone Titer: Degradation, Comprehensive Insect Physiology, Biochemistry and Pharmacology, Kerkut, G.A. and Gilbert, L.I., Eds., New York: Pergamon, 1985, vol. 7, pp. 431-472.

    Google Scholar 

  31. Khlebodarova, T.M., Gruntenko, N.E., Grenback, L.G., et al., A Comparative Analysis of Juvenile Hormone-Metabolizing Enzymes in Two Species of Drosophila during Development, Insect Biochem. Mol. Biol., 1996, vol. 26, pp. 829-835.

    Google Scholar 

  32. Gruntenko, N.E., Khlebodarova, T.M., Vasenkova, I.A., et al., Stress Reactivity of a Drosophila melanogaster Strain with Impaired Juvenile Hormone Action, J. Insect Physiol., vol. 46, pp. 451-456.

  33. Harshman, L.G., Loeb, A.M., and Johnson, B.A., Ecdysteroid Titers in Mated and Unmated Drosophila melanogaster Females, J. Insect Physiol., 1999, vol. 45, pp. 571-577.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, 630090 Russia

    I. Yu. Rauschenbach, N. E. Gruntenko, N. S. Chentsova, M. Zh. Sukhanova, E. V. Andreenkova & G. V. Glazko

  2. Division of Bioresource and Bioenviromnental Sciences, Graduate School, Kyushu University, Fukuoka, 812-8581 Japan

    A. Hirashima

Authors
  1. I. Yu. Rauschenbach
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. E. Gruntenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. N. S. Chentsova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Hirashima
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Zh. Sukhanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. E. V. Andreenkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. G. V. Glazko
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rauschenbach, I.Y., Gruntenko, N.E., Chentsova, N.S. et al. Regulation of Reproductive Function in DrosophilaFemales Due to Hormonal Interaction Under Stress Is Genetically Determined. Russian Journal of Genetics 37, 1041–1048 (2001). https://doi.org/10.1023/A:1011961514711

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1011961514711

Keywords

Search

Navigation