Abstract
The virulence spectrum of 112 isolates of Phytophthora clandestina collected from 56 sites in four subterranean clover-growing states in southern Australia was determined using differential cultivars of subterranean clover. Five races were detected, with race 0 in all states except New South Wales, race 1 in all states, race 2 only in Victoria, race 3 only in New South Wales, and race 4 in Victoria and Western Australia. The level of genotypic diversity among the different P. clandestina populations was investigated using five RAPD primers. Among 30 bands amplified, only two were polymorphic. This enabled identification of four multilocus RAPD genotypes. Three of the four genotypes occurred in all four states. Races 2 and 3 occurred with RAPD genotypes 1 and 2 only whereas races 0 and 1 occurred in all four multilocus RAPD genotypes. These results indicate that the pathogenicity spectrum of P. clandestina can change rapidly.
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References
Barbetti MJ (1994) Root rots of subterranean clover: A Western Australian perspective. In: Flett SP and Guppy W (eds) Technical workshop proceedings of Phytophthora root rot in subterranean clover1994 (pp 18-19),Agriculture Victoria, Tatura, Australia
Bhat RG and Schmitthenner AF (1993) Genetic crosses between physiologic races of Phytophthora sojae.Experimental Mycology17:122-129
Botstein D, White RL, Skolnick M and Davis RW (1980) Construction of a genetic linkage map in man using Restriction Fragment Length Polymorphisms.American Journal of Human Genetics 32:314-331
Dear BS, Murray GM and Flett SP (1994)Phytophthora root rot in subterranean clover.Agnote New SouthWales Agric9:1-4
Drenth A, Whisson SC, Maclean DJ, Irwin JAG, Obst NR and Ryley MJ (1996) The evolution of races of Phytophthora sojae in Australia.Phytopathology86:163-169
Erwin DC and Ribeiro OK (1998) Phytophthora diseases worldwide. American Phytopathological Society, St. Paul
Flett SP (1994) Studies on Phytophthora clandestina, the cause of tap root rot in subterranean clover. I. Evidence for physiological specialisation in Phytophthora clandestina.Australian Journal of Experimental Agriculture 34:1125-1129
Flett SP, Guppy W and Cook T (1994) Update on Phytophthora root rot research. In: Flett SP and Guppy W (eds) Technical workshop proceedings of Phytophthora root rot in subterranean clover1994 (pp 1-3)Agriculture Victoria, Tatura, Australia
Flett SP, Taylor PA and Wakley V (1993) A method for the greenhouse evaluation of subterranean clover (Trifolium subterraneum) breeding lines for Phytophthora clandestina root rot.Proceedings of the 17th International Grassland Congress, Session 22:70
Förster H, Tyler BM and Coffey MD (1994) Phytophthora sojae races have arisen by clonal evolution and by rare outcrosses. Molecular Plant-Microbe Interactions7:780-791
Goodwin SB (1997) The population genetics of Phytophthora. Phytopathology 87:462-473
Goodwin SB, Spielman LJ, Matuszak JM, Bergeron SN and Fry WE (1992) Clonal diversity and genetic differentiation of Phytophthora infestans populations in northern and centralMexico. Phytopathology82:955-961
Goodwin SB, Sujkowski LS and Fry WE (1995) Rapid evolution of pathogenicity within clonal lineages of the potato late blight disease fungus.Phytopathology85: 669-676
Greenhalgh FC (1992) Studies on the aetiology and significance of root diseases of subterranean clover, and the histopathology and control of Phytophthora root rot. PhD thesis. La Trobe University, Melbourne
Greenhalgh FC and Clarke RG (1985) Use of fungicides to study significance and etiology of root rot of subterranean clover in dryland pastures of Victoria. In: Parker CA, Moore KJ, Wong PTW, Rovira AD and Kollmorgen JF (eds) Ecology and management of soilborne plant pathogens (pp 234-236) American Phytopathological Society, St. Paul
Greenhalgh FC, de Boer RF, Merriman PR, Hepworth G and Keane PJ (1994) Control of Phytophthora root rot of irrigated subterranean clover with potassium phosphonate in Victoria, Australia.Plant Pathology43:1009-1019
Greenhalgh FC and Taylor PA (1985) Phytophthora clandestina, cause of severe taproot rot of subterranean clover in Victoria, Australia.Plant Disease69:1002-1004
Lee SB and Taylor JW (1990) Isolation of DNA from fungal mycelia and single spores. In: Innis MA, Gelfand DH, Sninsky JJ and White TJ (eds) PCR protocols: a guide to methods and applications (pp 282-287) Academic Press, London
McDermott JM and McDonald BA (1993) Gene flow in plant pathosystems.Annual Review of Phytopathology31:353-373
Purwantara A, Flett SP and Keane PJ (1995) Variation in pathogenicity and virulence of Phytophthora clandestina on four cultivars of subterranean clover.Australian Journal of Experimental Agriculture35:717-724
Purwantara A, Flett SP and Keane PJ (1996a) Resistance of 19 cultivars of subterranean clover to 4 races of Phytophthora clandestina.Euphytica 91:351-358
Purwantara A, Flett SP and Keane PJ (1996b) Bioassay and baiting methods for determining occurrence of races of Phytophthora clandestina in soil.Australian Journal of Experimental Agriculture 36:815-822
Purwantara A, Flett SP and Keane PJ (1998) Colonization of roots of subterranean clover cultivars by virulent and avirulent races of Phytophthora clandestina.Plant Pathology47:67-72
Smiley RW, Taylor PA, Clarke RG, Greenhalgh FC and Trutmann P (1986) Simulated soil and plant management effects on root rots of subterranean clover.Australian Journal of Agricultural Research37:633-645
Stoddart JA and Taylor JF (1988) Genotypic diversity: Estimation and prediction in samples.Genetics118:705-711
Taylor PA (1984) An unusual Phytophthora associated with root rot of subterranean clover.Plant Disease68:450
Taylor PA, Barbetti MJ and Wong DH (1985a) Occurrence of Phytophthora clandestina in Western Australia.Plant Protection Quarterly1: 57-58
Taylor PA, Clarke RG, Kelly K and Smiley R (1985b) Root rot of irrigated subterranean clover in northern Victoria: significance and prospects for control. In: Parker CA, Moore KJ, Wong PTW, Rovira AD and Kollmorgen JF (eds) Ecology and management of soilborne plant pathogens (pp 271-273) American Phytopathological Society, St. Paul
Taylor PA and Greenhalgh FC (1987) Significance, causes and control of root rots of subterranean clover. In: Wheeler JL, Pearson CJ and Robards GE (eds)Temperate pastures: their production, use and management (pp 249-251)Australian Wool Corporation/Commonwealth Scientific and Industrial Research Organisation, Melbourne
Taylor PA, Pascoe IG and Greenhalgh FC (1985c) Phytophthora clandestina sp. nov. in roots of subterranean clover.Mycotaxon 22:77-85
Whisson SC, Drenth A, Maclean DJ and Irwin JAG (1994) Evidence for outcrossing in Phytophthora sojae and linkage of a DNA marker to two avirulence genes.Current Genetics27: 77-82
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA and Tingey S (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.Nucleic Acids Research18: 6531-6536
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Purwantara, A., Drenth, A., Flett, S.P. et al. Diversity of Phytophthora Clandestina Isolated From Subterranean Clover in Southern Australia: Analysis of Virulence and RAPD Profiles. European Journal of Plant Pathology 107, 305–311 (2001). https://doi.org/10.1023/A:1011272419130
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DOI: https://doi.org/10.1023/A:1011272419130