biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 44:189-193, 2001 | DOI: 10.1023/A:1010282805522

Enhancement of Regeneration Potential and Variability by γ-Irradiation in Cultured Cells of Scilla Indica

B. Chakravarty1, S. Sen1
1 Centre of Advanced Study, Department of Botany, University of Calcutta, Calcutta -, India

Induced mutagenesis in callus tissues was studied in the medicinal plant Scilla indica irradiated with different doses of γ-radiation ranging from 2.5 to 20 Gy. Low doses accelerated the cell division and growth rate of the tissues whereas high doses repressed growth rate and resulted in lethality of tissues. Various cytological and chromosomal abnormalities were observed in the irradiated calli, the degree of which depended upon the dosage. Low doses of irradiation also promoted the regenerating capacity of the calli tissues and plants regenerating from them exhibited better growth and vigour compared to normal plants. High doses led to loss of regenerating capacity and promoted formation of malformed and stunted plants. Cytological study of regenerants revealed both diploid and mixoploid plants but no tetraploids were obtained.

Keywords: callus; dosage; diploids; mixoploids
Subjects: gamma-radiation, mutagenesis, in vitro culture; in vitro culture, micropropagation, regeneration; mutagenesis, in vitro culture, g-radiation; ploidy level; Scilla indica

Published: June 1, 2001  Show citation

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Chakravarty, B., & Sen, S. (2001). Enhancement of Regeneration Potential and Variability by γ-Irradiation in Cultured Cells of Scilla Indica. Biologia plantarum, 44(2), 189-193. doi: 10.1023/A:1010282805522
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