Abstract
Thirty-three species of marine invertebrates from the Sea of Japan were analyzed for contents of fucoidan hydrolases and some glycosidases. Fucoidan hydrolase activity was assessed by examining the effect of animal tissue extracts on fucoidans from the two brown seaweeds Laminaria cichorioides and Fucus evanescens, which have different structural characteristics. The activity of glycosidases (β-glucosidase, β-galactosidase, α-fucosidase, and α-mannosidase) was determined using p-nitrophenyl derivatives of sugars as substrates. It was found that glycosidases and fucoidan hydrolases of different specificities are fairly widely distributed among marine invertebrates. Mollusks and some species of echinoderms and arthropods showed the highest enzymatic activity. This research will enable us to choose organisms for the separation and study of fucoidan hydrolases and glycosidases, which may be useful in determining the structure of fucoidans.
Similar content being viewed by others
REFERENCES
Bakunina, I.Yu., Ivanova, E.P., Nedashkovskaya, O.I., et al., Search for Producers of α-Galactosidases among Marine Bacteria of the Genus Alteromonas, Prikl. Biokhim. Mikrobiol., 1996, vol. 32, pp. 624–628.
Bakunina, I.Yu., Sova, V.V., Nedashkovskaya O.I., et al., α-Galactosidases of the Marine Bacterium Pseudoalteromonas sp. KMM 701, Biokhimiya, 1998, vol. 63, pp. 1420–1427.
Zvyagintseva, T.N., Sova, V.V., Bakunina, I.Yu., et al., Marine Organisms as Sources of Biologically Active Polysaccharides, Polysaccharide Hydrolases with Unique Specificity and Their Inhibitors, Khimiya v interesakh ustoichivogo razvitiya (Chemistry in the Interests of Sustainable Development), 1998, vol. 6, pp. 417–426.
Alexander, C.G., Cutler, R.L., and Yellowless, D., Studies on the Composition and Enzyme Content of the Crystalline Style of Telescopium telescopium (L.) (Gastropoda), Comp. Biochem. Physiol., Ser. B., 1979, vol. 64, pp. 83–89.
Elyakova L.A., Sova, V.V., and Vaskovsky, V.E., Cellulase of Marine Mollusc (Littorina sp.), Biochim. Biophys. Acta, 1968, vol. 167, pp. 462–464.
Furukawa, S., Fujikawa, T., Koga, D., et al., Purification and Some Properties of Exotype Fucoidanases from Vibrio sp. N-5, Biosci. Biotechnol. Biochem., 1992, vol. 56, pp. 1829–1834.
Kitamura K., Matsuo, M., and Yasui, T., Enzymatic Degradation of Fucoidan by Fucoidanase from the Hepatopancreas of Patinopecten yessoensis, Biosci. Biotechnol. Biochem., 1992, vol. 56, pp. 490–494.
Kozlovskaya, E.P. and Vaskovsky, V.E., A Comparative Study of Proteinases of Marine Invertebrates, Comp. Biochem. Physiol., 1970, vol. 34, pp. 137–142.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., et al., Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem., 1951, vol. 193, pp. 265–275.
Nagumo, T. and Nishino, T., Fucan Sulfates and Their Anticoagulant Activities, Polysaccharides in Medicinal Applications, New York, Basel: Marcel Dekker, 1997, pp. 545–574.
Nelson, N., A Photometric Adaptation of the Somogyi Method of Determination of Glucose, J. Chem., 1944, vol. 153, pp. 375–381.
Sasaki, K., Sakai, T., Kojima, K., et al., Partial Purification and Characterization of an Enzyme Releasing 2-Sulfo-α-L-fucopyranosyl(1→2)pyridylaminated Fucose from a Sea Urchin, Strongylocentrotus nudus, Biosci. Biotechnol. Biochem., 1996, vol. 60, no. 4, pp. 666–668.
Sova, V.V., Elyakova, L.A., and Vaskovsky, V.E., The Distribution of Laminarinases in Marine Invertebrates, Comp. Biochem. Physiol., 1970, vol. 32, pp. 459–464.
Thanassi, N.M. and Nakada, H.I., Enzymatic Degradation of Fucoidan by Enzymes from the Hepatopancreas of Abalone, Haliotis species, Arch. Biochem. Biophys., 1967, vol. 118, pp. 172–177.
Yamamoto, K., Yasunobu, T., Kumagai, H., et al., Induction and Purification of α-L-fucosidase from Fusarium oxysporum, Agr. Biol. Chem., 1986, vol. 50, pp. 1689–1695.
Zvyagintseva, T.N., Shevchenko, N.M., Popivnich, I.B., et al., A New Procedure for the Separation of Water-soluble Polysaccharides from Brown Seaweeds, Carbohydr. Res., 1999, vol. 322, pp. 32–39.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Burtseva, Y.V., Kusaikin, M.I., Sova, V.V. et al. Distribution of Fucoidan Hydrolases and Some Glycosidases among Marine Invertebrates. Russian Journal of Marine Biology 26, 453–456 (2000). https://doi.org/10.1023/A:1009459107956
Issue Date:
DOI: https://doi.org/10.1023/A:1009459107956