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PRINS Analysis of the Telomeric Sequence in Seven Lemurs

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Abstract

We examined the chromosomal localization of the telomeric sequence, (TTAGGG)n, in seven species of the lemurs and one greater galago, as an outgroup, using the primed in-situ labeling (PRINS) technique. As expected, the telomeric sequence was identified at both ends of all chromosomes of the eight prosimians. However, six species showed a signal at some pericentromeric regions involving constitutive heterochromatin as well. The pericentromeric region of chromosome 1 of Verreaux's sifaka (Propithecus verreauxi verreauxi) was labeled with a large and intense signal. The range of the signal considerably exceeded the area of DAPI positive heterochromatin. On the other hand, in the five lemurs, a large signal was detected also in the short arm of acrocentric chromosomes. Acquisition of the large block of the telomeric sequence into the acrocentric short arm might be interpretable in terms of the tandem growth of the heterochromatic short arm and the reciprocal translocation between heterochromatic short arms involving the telomeric sequence. Subsequently, it was postulated that meta- or submetacentric chromosomes possessing the telomeric sequence at the pericentromeric region could be formed by centric fusion between such acrocentric chromosomes.

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Authors and Affiliations

  1. Primate Research Insitute, Kyoto University, Inuyama, Aichi, 484-8506, Japan

    Yasuhiro Go & Yoshi Kawamoto

  2. Parc Botanique et Zoologique de Tsimbazaza, B.P. 4096, Antananarivo, 101, Madagascar

    Gilbert Rakotoarisoa & Albert Randrianjafy

  3. Center for African Area Studies, Kyoto University, 46 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto, 606-8304, Japan

    Naoki Koyama

  4. Primate Research Insitute, Kyoto University, Inuyama, Aichi, 484-8506, Japan

    Hirohisa Hirai

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  1. Yasuhiro Go
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  2. Gilbert Rakotoarisoa
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  6. Hirohisa Hirai
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Go, Y., Rakotoarisoa, G., Kawamoto, Y. et al. PRINS Analysis of the Telomeric Sequence in Seven Lemurs. Chromosome Res 8, 57–65 (2000). https://doi.org/10.1023/A:1009279203886

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