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Separate and variably shaped chromosome arm domains are disclosed by chromosome arm painting in human cell nuclei

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Abstract

Fluorescence in situ hybridization (FISH) with microdissection probes from human chromosomes 3 and 6 was applied to visualize arm and subregional band domains in human amniotic fluid cell nuclei. Confocal laser scanning microscopy and quantitative three-dimensional image analysis showed a pronounced variability of p- and q-arm domain arrangements and shapes. Apparent intermingling of neighbouring arm domains was limited to the domain surface. Three-dimensional distance measurements with pter and qter probes supported a high variability of chromosome territory folding.

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Authors and Affiliations

  1. Center of Molecular Biology, University of Heidelberg, Im Neuenheimer Feld 282, 69120, Heidelberg, Germany

    Steffen Dietzel

  2. Institute of Human Genetics, University of Heidelberg Heidelberg, Germany

    Anna Jauch, Dirk Kienle, Guoquiong Qu, Michael Bittner, Paul S. Meltzer, Jeffrey M. Trent & Thomas Cremer

  3. Interdisciplinary Center of Scientific Computing, University of Heidelberg, Heidelberg, Germany

    Heidi Holtgreve-Grez

  4. Division of Biophysics of Macromolecules, Deutsches Krebsforschungszentrum, Heidelberg, Germany

    Roland Eils

  5. Laboratory of Cancer Genetics, National Human Genome Research Institute, National Institutes of Health, Bethesda, USA

    Christian Munkel

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  1. Steffen Dietzel
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  2. Anna Jauch
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  3. Dirk Kienle
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  4. Guoquiong Qu
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  5. Heidi Holtgreve-Grez
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  6. Roland Eils
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  7. Christian Munkel
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  8. Michael Bittner
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  9. Paul S. Meltzer
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  11. Thomas Cremer
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Dietzel, S., Jauch, A., Kienle, D. et al. Separate and variably shaped chromosome arm domains are disclosed by chromosome arm painting in human cell nuclei. Chromosome Res 6, 25–33 (1998). https://doi.org/10.1023/A:1009262223693

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  • DOI: https://doi.org/10.1023/A:1009262223693

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