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Use of Chlorella vulgaris as a carotenoid source for rainbow trout: effect of dietary lipid content on pigmentation, digestibility and retention in the muscle tissue

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Abstract

Apparent digestibility, deposition and retention of carotenoids in the muscle of rainbow trout, Oncorhynchus mykiss, were investigated comparing the feeding of pigments from Chlorella vulgaris against commercially available pigments at two different total lipid contents (15% and 20% lipid). Algal biomass (ALG) was included in rainbow trout diets and muscle pigmentation was compared to that obtained in trout fed diets containing a 5:3 mixture of canthaxanthin and astaxanthin (MIX) (reflecting the relative concentrations of these carotenoids in the dry alga) or those fed a diet containing astaxanthin only (AST). Apparent digestibilities of pigments and nutrients were determined by the indirect method, using Cr2 O3 as indicator, and the colour intensity and pigment concentration were assessed in the muscle, using the Roche colour card for salmonids and UV-vis spectrophotometry. After 6 weeks feeding, colour intensity was similar for the various pigment sources, achieving levels 12 to 13, yet significantly higher in fish fed the diet with the higher lipid content (≈ 20%) (p<0.05). Pigment concentration in the muscle was also higher in the fish fed the high-fat diet. Muscle pigment concentrations were similar for fish fed diets ALG and MIX, and over 1.5 times higher than for diet AST (p<0.05) after 6 weeks. Apparent digestibility of dry matter, crude protein, lipid, total energy and specific carotenoid concentrations were also measured. Increased dietary fat content was shown to increase the deposition and the retention of carotenoids in muscle, and the difference increased with time (deposition increase of 10–20% at week 3 and 30–40% at week 6 and retention increase of 10–15% at week 3 and 30% at week 6). Pigment digestibility also apparently increases (10–20%) under those conditions despite the fact that no significant effects in terms of apparent digestibility increase were found for dry matter, protein, lipids or energy. © Rapid Science Ltd. 1998

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Authors and Affiliations

  1. Estrada do Paço do Lumiar, Instituto Nacional de Engenharia e Tecnologia Industrial - INETI, Lisboa-Codex, Portugal

    Luisa Gouveia

  2. Unité Mixte INRA-IFREMERSaint, Lab°. de Nutrition des Poissons, Pée-sur-Nivelle, France

    Georges Choubert

  3. Instituto de Ciências Biomédicas, Universidade do Porto, Porto, Portugal

    Emídio Gomes

  4. Universidade de Trás-os-Montes e Alto Douro, Vila-Real, Portugal

    Paulo Rema

  5. Lab°. de Enga Bioquímica, Secção de Biotecnologia, Instituto Superior Técnico, Av. Rovisco Pais, Lisboa-Codex, Portugal

    José Empis

Authors
  1. Luisa Gouveia
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  2. Georges Choubert
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  3. Emídio Gomes
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  4. Paulo Rema
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  5. José Empis
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Gouveia, L., Choubert, G., Gomes, E. et al. Use of Chlorella vulgaris as a carotenoid source for rainbow trout: effect of dietary lipid content on pigmentation, digestibility and retention in the muscle tissue. Aquaculture International 6, 269–279 (1998). https://doi.org/10.1023/A:1009251714573

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