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Cold-Shock 310-kD Protein Uncouples Oxidative Phosphorylation in Plant Mitochondria

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Abstract

We studied the effects of cold-shock 310-kD protein (CSP310) isolated from winter rye seedlings on the energetic activity of plant mitochondria. CSP310 was shown to enhance nonphosphorylating respiration and uncoupled oxidative phosphorylation in isolated mitochondria. The uncoupling effect was enhanced with increasing protein concentration. An antibody against CSP310 interfered with the uncoupling effect of CSP310. Free fatty acids were not evidently involved in uncoupling. The physiological role of uncoupling between oxidation and phosphorylation during plant adaptation to low temperatures is discussed.

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REFERENCES

  1. Lindquist, S., The Heat Shock Response, Annu. Rev. Biochem., 1986, vol. 55, pp. 1151-1191.

    Google Scholar 

  2. Guy, C., Cold Acclimation and Freezing Stress Tolerance: Role of Protein Metabolism, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1990, vol. 41, pp. 187-223.

    Google Scholar 

  3. Kurkela, S. and Frank, M., Cloning and Characterization of Cold-and ABA-Inducible Arabidopsis Gene, Plant Mol. Biol., 1990, vol. 15, pp. 137-144.

    Google Scholar 

  4. Guy, C.L., Huber, J.L.A., and Huber, S.C., Sucrosephosphate Synthase and Sucrose Accumulation at Low Temperature, Plant Physiol., 1992, vol. 100, pp. 502-508.

    Google Scholar 

  5. Krishna, P., Sacco, M., Cherutti, J.F., and Hill, S., Cold-Induced Accumulation of hsp 90 Transcripts in Brassica napus, Plant Physiol., 1995, vol. 107, pp. 915-923.

    Google Scholar 

  6. Close, T.J., Dehydrins: Emergence and Biochemical Role of a Family of Plant Dehydratation Proteins, Physiol. Plant., 1996, vol. 97, pp. 795-803.

    Google Scholar 

  7. Griffith, M., Antikainen, M., Hon, W.C., Pihakaski, M.K., Yu, X.M., Chun, J.U., and Yang, D.S., Antifreeze Proteins in Winter Rye, Physiol. Plant., 1997, vol. 100, pp. 327-332.

    Google Scholar 

  8. Griffith, M., Ala, P., Yang, D.S., Hon, W.C., and Moffatt, B.A., Antifreeze Protein Produced Endogenously in Winter Rye, Plant Physiol., 1992, vol. 100, pp. 593-596.

    Google Scholar 

  9. Jezek, P., Engstova, H., Zackova, M., Vercesi, A.E., Costa, A.D., Arrud, O., and Garlid, K.D., Fatty Acid Cycling Mechanism and Mitochondrial Uncoupling Protein, Biochim. Biophys. Acta, 1998, vol. 1365, pp. 319-327.

    Google Scholar 

  10. Maia, I.G., Benedetti, C.E., Leite, A., Turcinelli, S.R., Vercesi, A.E., and Arruda, P., AtPUMP: An Arabidopsis Gene Encoding a Plant Uncoupling Mitochondrial Protein, FEBS Lett., 1998, vol. 429, pp. 403-406.

    Google Scholar 

  11. Kolesnichenko, A.V., Borovskii, G.B., Voinikov, V.K., Misharin, S.I., and Antipina, A.I., Antigen from Winter Rye Accumulated under Low Temperature, Fiziol. Rast. (Moscow), 1996, vol. 43, pp. 894-899 (Russ. J. Plant Physiol., Engl. Transl.).

    Google Scholar 

  12. Kolesnichenko, A.V., Ostroumova, E.A., Zykova, V.V., and Voinikov, V.K., The Comparison of Proteins with Immunochemical Affinity to Stress Protein 310 kD in Cytoplasmatic Proteins of Winter Rye, Winter Wheat, Elymus and Maize, J. Therm. Biol., 1999, vol. 24, pp. 211-215.

    Google Scholar 

  13. Kolesnichenko, A.V., Zykova, V.V., and Voinikov, V.K., A Comparison of the Immunochemical Affinity of Cytoplasmic, Mitochondrial and Nuclear Proteins of Winter Rye (Secale cereale L.) to a 310 kD Stress Protein in Control Plants and during Exposure to Cold Stress, J. Therm. Biol., 2000, vol. 25, pp. 203-209.

    Google Scholar 

  14. Voinikov, V., Pobezhimova, T., Kolesnichenko, A., Varakina, N., and Borovskii, G., Stress Protein 310 kD Affects the Energetic Activity of Plant Mitochondria under Hypothermia, J. Therm. Biol., 1998, vol. 23, pp. 1-4.

    Google Scholar 

  15. Voinikov, V.K., Temperaturnyi stress i mitokhondrii rastenii (Temperature Stress and Plant Mitochondria), Novosibirsk: Nauka, 1987.

    Google Scholar 

  16. Skulachev, V.P., Energetika biologicheskikh membran (Energetics of Biological Membranes), Moscow: Nauka, 1989.

    Google Scholar 

  17. Wilson, R.H. and Smith, B.N., Uncoupling of Sauromatum spadix Mitochondria as a Mechanism of Thermogenesis, Z. Pflanzenphysiol., 1971, vol. 65, pp. 124-129.

    Google Scholar 

  18. Vercesi, A.E., Martin, I.S., Silva, M.A.P., Leite, H.M.F., Cuccovia, I.M., and Chaimovich, H., PUMPing Plants, Nature, 1995, vol. 375, p. 24.

    Google Scholar 

  19. Laloi, M., Klein, M., Reismeier, J.W., Muller-Rober, B., Fkeury, C., Bouilland, F., and Rocguier, D., A Plant Cold-Induced Uncoupling Protein, Nature, 1997, vol. 389, pp. 135-136.

    Google Scholar 

  20. Kolesnichenko, A., Zykova, V., Grabel'nych, O., Sumina, O., Pobezhimova, T., and Voinikov, V., Screening of Mitochondrial Proteins in Winter Rye, Winter Wheat, Elymus and Maize with an Immunochemical Affinity to the Stress Protein 310 kD and Their Intramitochondrial Localization in Winter Wheat, J. Therm. Biol., 2000, vol. 25, pp. 245-249.

    Google Scholar 

  21. Voinikov, V.K., Konstantinov, Yu.M., and Negruk, V.I., Geneticheskie funktsii mitokhondrii rastenii (Genetic Functions of Plant Mitochondria), Novosibirsk: Nauka, 1991.

    Google Scholar 

  22. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.L., Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem., 1951, vol. 193, pp. 265-275.

    Google Scholar 

  23. Estabrook, R.W., Mitochondrial Respiratory Control and the Polarographic Measurement of ADP: O Ratio, Methods Enzymol., 1967, vol. 10, pp. 41-47.

    Google Scholar 

  24. Borovskii, G., Voinikov, V., and Kolesnichenko, A., The Effect of Hypothermia on the Content of 310 kD Stress Protein in Seedlings of Winter Rye and Wheat, J. Therm. Biol., 1999, vol. 24, pp. 91-95.

    Google Scholar 

  25. Voinikov, V.K., Kolesnichenko, A.V., Pobezhimova, T.P., and Varakina, N.N., The First and Sixth Chromosomes of Soft Winter Wheat D Genome Control the Synthesis of 310-kD Cold-Shock Protein, Fiziol. Rast. (Moscow), 1998, vol. 45, pp. 688-692 (Russ. J. Plant Physiol., Engl. Transl.).

    Google Scholar 

  26. Palou, A., Pico, C., Bonet, M.L., and Oliver, P., The Uncoupling Protein, Thermogenin, Int. J. Biochem. Cell Biol., 1998, vol. 30, pp. 7-11.

    Google Scholar 

  27. Voinikov, V.K. and Korzun, A.M., Temperature of Winter Wheat Tissues during Cold Shock, Izv. Sib. Otd. Akad. Nauk SSSR, Ser. Biol. Nauk, 1984, no. 2, pp. 22-25.

    Google Scholar 

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Authors and Affiliations

  1. Siberian Institute of Plant Physiology and Biochemistry, Siberian Division, Russian Academy of Sciences, ul. Lermontova 132, Irkutsk, 664033, Russia

    V. K. Voinikov, O. I. Grabel'nykh, A. V. Kolesnichenko & T. P. Pobezhimova

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  1. V. K. Voinikov
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  2. O. I. Grabel'nykh
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  3. A. V. Kolesnichenko
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  4. T. P. Pobezhimova
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Voinikov, V.K., Grabel'nykh, O.I., Kolesnichenko, A.V. et al. Cold-Shock 310-kD Protein Uncouples Oxidative Phosphorylation in Plant Mitochondria. Russian Journal of Plant Physiology 48, 89–94 (2001). https://doi.org/10.1023/A:1009058800551

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