Abstract
We studied the localization of polypeptides immunochemically related to subunits of cold-shock 310-kD protein from winter rye (Secale cerealeL.) in mitochondria and submitochondrial structures of winter wheat (Triticum aestivumL.) seedlings. Polypeptides were separated by SDS-PAGE and probed with the antibody against 310-kD protein from rye seedlings. Wheat mitochondria contained the following polypeptides cross-reacting with this antibody: 66, 60, 55, and 23 kD in the inner membrane; 60 and 58 kD in the outer membrane; and 66 and 55 kD in the matrix.
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REFERENCES
Houde, M., Daniel, C., Lachapelle, M., Allard, F., Laliberte, S., and Sarhan, F., Immunolocalization of Freezing-Tolerance-Associated Proteins in the Cytoplasm and Nucleoplasm of Wheat Crown Tissues, Plant J., 1995, vol. 8, pp. 583–593.
Griffith, M., Antikainen, M., Hon, W.C., Pihakaskimaunsbach, K., Yu, X.M., Chun, J.U., and Yang, D.S.C., Antifreeze Proteins in Winter Rye, Physiol. Plant., 1997, vol. 100, pp. 327–332.
Skulachev, V.P., Energetika biologicheskikh membran (Energy Metabolism of Biomembranes), Moscow: Nauka, 1989.
Jezek, P., Engstova, H., Zackova, M., Vercesi, A.E., Costa, A.D.T., Arruda, P., and Garlid, K.D., Fatty Acid Cycling Mechanism and Mitochondrial Uncoupling Proteins, Biochim. Biophys. Acta, 1998, vol. 1365, pp. 319–327.
Vercesi, A.E., Matins, I.S., Silva, M.A.P., Leite, H.M.F., Cuccovia, I.M., and Chaimovich, H., PUMPing Plants, Nature, 1995, vol. 375, p. 24.
Laloi, M., Klein, M., Riesmeier, J.W., Muller-Rober, B., Fleury, C., Bouilland, F., and Ricguier, D., A Plant Cold-Induced Uncoupling Protein, Nature, 1997, vol. 389, pp. 135–136.
Kolesnichenko, A.V., Borovskii, G.B., Voinikov, V.K., Misharin, S.I., and Antipina, A.I., Antigen from Winter Rye Accumulated under Low Temperature, Fiziol. Rast. (Moscow), 1996, vol. 43, pp. 894–899 (Russ. J. Plant Physiol., Engl. Transl.).
Voinikov, V., Pobezhimova, T., Kolesnichenko, A., Varakina, N., and Borovskii, G., Stress Protein 310 kD Affects the Energetic Activity of Plant Mitochondria under Hypothermia, J. Therm. Biol., 1998, vol. 23, pp. 1–4.
Pobezhimova, T.P., Kolesnichenko, A.V., Voinikov, V.K., Varakina, N.N., and Borovskii, G.B., Stress Protein 310 kD Affects the Energetic Activity of Plant Mitochondria under Hypothermia, Dokl. Akad. Nauk, 1996, vol. 350, pp. 715–718.
Voinikov, V.K., Konstantinov, Yu.M., and Negruk, V.I., Geneticheskie funktsii mitokhondrii rastenii (Genetic Function of Plant Mitochondria), Novosibirsk: Nauka, 1991.
Douce, R., Mitochondria in Higher Plants: Structure, Function and Biogenesis, London: Academic, 1985.
Davy de Virville, J., Aaron, I., Alin, M.F., and Moreau, F., Isolation and Properties of Mitochondria from Arabidopsis thaliana Cell Suspension Cultures, Plant Physiol. Biochem., 1994, vol. 32, pp. 159–166.
Guillot-Salomon, T., Remy, R., Cantrel, C., Demandre, C., and Moreau, F., Phospholipids and Polypeptides in the Outer Membrane of Maize Mitochondria, Phytochemistry, 1997, vol. 44, pp. 29–43.
Azarashvili, T.S., Kudin, A.P., Polteva, N.A., Kudzina, L.Yu., and Evtodienko, Yu.V., Polypeptides Composition of Submitochondrial Fraction from Normal Liver Tissue and Saidel Hepatome, Biokhimiya, 1997, vol. 62, pp. 833–841.
Tolbert, N.E., Isolation of Sub-Cellular Organelles of Metabolism on Isopicnic Sucrose Gradients, Methods Enzymol., 1974, vol. 31, pp. 734–746.
Moore, A.L. and Proudlove, M.O., Mitochondria and Sub-Mitochondrial Particles, Isolation of Membranes and Organelles from Plant Cells, Hall, T.L. and Moore, A.L., Eds., London: Academic, 1983, pp. 153–184.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem., 1957, vol. 193, pp. 265–275.
Voinikov, V.K. and Rudikovskii, A.V., Association of Maize Heat-Shock Proteins with Mitochondria in vivo and in vitro, Fiziol. Rast. (Moscow), 1988, vol. 35, pp. 542–547 (Sov. Plant Physiol., Engl. Transl.).
Whelan, J., Knorpp, C., and Glaser, E., Sorting of Precursor Protein between Isolated Spinach Leaf Mitochondria and Chloroplasts, Plant Mol. Biol., 1990, vol. 14, pp. 977–982.
Borovskii, G.B. and Voinikov, V.K., Localization of Low Molecular Weight Heat-Shock Proteins inside and on the Outer Surface of Mitochondria, Fiziol. Rast. (Moscow), 1993, vol. 40, pp. 596–598 (Russ. J. Plant Physiol., Engl. Transl.).
Esen, A., A Simple Method for Quantitative, Semiquantitative and Qualitative Assay of Protein, Anal. Biochem., 1978, vol. 89, pp. 264–273.
Laemmli, U.K., Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4, Nature, 1970, vol. 227, pp. 680–685.
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Pobezhimova, T.P., Grabel'nykh, O.I., Kolesnichenko, A.V. et al. Localization of Proteins Immunologically Related to Subunits of Stress 310-kD Protein in Winter Wheat Mitochondria. Russian Journal of Plant Physiology 48, 204–209 (2001). https://doi.org/10.1023/A:1009052101017
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DOI: https://doi.org/10.1023/A:1009052101017