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Fluorescent derivatives of bovine neurotensin 8–13 fragment

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Abstract

Fluorescent derivatives of bovine neurotensin 8–13 fragment were prepared. For N-terminal labelling, 4-[7-hydroxycoumaryl]acetic acid (Hca), 4-[7-methoxycoumaryl]acetic acid (Mca) and 2-amino-3-[4-[7-methoxycoumaryl]]propionic acid (Amp) were used while the C-terminus of the peptide chain was elongated with Amp. The fluorescence excitation and emission spectra of the peptide derivatives were studied. Hca- and Mca/Amp-derivatives were easily distinguishable because of the 60 nm shift of their emission maxima. Compared with the natural sequence, the presence of an N-terminal label did not influence the biological potency in a longitudinal muscle strip of guinea-pig ileum, while labelling at the C-terminus considerably reduced the activity of the peptide.

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Authors and Affiliations

  1. Department of Organic Chemistry, Eötvös Loránd University, P.O. Box 32, H-1518, Budapest 112, Hungary

    Péter Kele, Eszter Illyés & Ferenc Sebestyén

  2. Hungarian Academy of Sciences, Research Group of Peptide Chemistry, P.O. Box 32, H-1518, Budapest 112, Hungary

    Gábor Mezö

  3. Hungarian Academy of Sciences, Institute of Experimental Medicine, P.O. Box 67, H-1450, Budapest, Hungary

    Margit Dóda & András D. Kaposi

  4. Gedeon Richter Pharmaceutical Works, Ltd., P.O. Box 27, H-1475, Budapest 10, Hungary

    Marianna Mák

Authors
  1. Péter Kele
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  2. Eszter Illyés
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  3. Gábor Mezö
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  4. Margit Dóda
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  5. Marianna Mák
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  6. András D. Kaposi
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  7. Ferenc Sebestyén
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Kele, P., Illyés, E., Mezö, G. et al. Fluorescent derivatives of bovine neurotensin 8–13 fragment. Letters in Peptide Science 6, 235–238 (1999). https://doi.org/10.1023/A:1008914513649

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  • DOI: https://doi.org/10.1023/A:1008914513649

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