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Effects of different levels of CO2 on photosynthesis and cell components of the red alga Porphyra leucosticta

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Abstract

Photosynthesis and cell composition of Porphyraleucosticta discs grown at low (< 0.0001% in air), current (control) and high (1% CO2 in air)inorganic carbon (Ci) concentrations were analyzed. Carbohydrate content in discs grown at high Ci increased (15.1 mg g-1 FW) with respect to the control (6.4 mg g FW-1), whereas soluble protein content decreased to one-third (5.6 to2.1 mg g-1 FW). Carbohydrate content was unaffected and soluble protein slightly increased in discs grown at low Ci. As a consequence of these changes, a lower C/N molar ratio (8.6) was found in the discs grown at low compared to high Ci(12.4). Nitrate reductase activity increased at high Ci from 0.3 ± 0.2 to 1.7 ± 0.4 μmolNO2 - g-1 FW h-1indicating that reduction and assimilation of nitrate were uncoupled. The response of photosynthesis to increasing irradiance, estimated from O2evolution vs. irradiance curves, was affected by the treatments. Maximum quantum yield (Φ O2°) and effective quantum yield (Φ O2) at 150 μmol photon m-2s-1 decreased by 20% and 50%, respectively, at low Ci. These differences could be due to changes in photosynthetic electron flow between PSII and PSI. Treatments also produced changes in maximal (Fv/Fm) and effective (ΔF/Fm′)quantum yield for photosystem II charge separation.

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References

  • Aizawa K, Miyachi S (1986) Carbonic anhydrase and CO2 concentrating mechanisms in microalgae and cyanobacteria. FEMS Microbiol. Rev. 39: 215–233.

    Article  CAS  Google Scholar 

  • Axelsson L, Uusitalo J, Ryberg H (1991) Mechanisms for concentrating and storage of inorganic carbon in marine macroalgae. In García-Reina G, Pedersén M (eds), Seaweed Cellular and Biotechnology. Physiology and Intensive Cultivation. COST-48, Universidad de las Palmas de Gran Canaria, Spain: 185–198.

    Google Scholar 

  • Badger MR, Kaplan A, Berry JA (1980) Internal inorganic carbon pool of Chlamydomonas reinhardtii. Plant. Physiol. 66: 407–413.

    PubMed  CAS  Google Scholar 

  • Badour SS, Irvine BR (1990) Activities of photosystems I and II in Chlamydomonas segnis adapted and adapting to air and airenriched with carbon dioxide. Bot. Acta 103: 149–154.

    CAS  Google Scholar 

  • Beer S, Eshel A (1985) Determining phycoerythrin and phycocyanin concentrations in aqueous crude extracts of red algae. Aust. J. mar. freshwat. Res. 36: 785–792.

    Article  CAS  Google Scholar 

  • Björk M, Haglund K, Ramazanov Z, Pedersén M (1993) Inducible mechanisms for HCO3- utilization and repression of photorespiration in protoplast and thalli of three species of Ulva (Chlorophyta). J. Phycol. 29: 166–173.

    Article  Google Scholar 

  • Bowes GW (1993) Facing the inevitable: Plants and increasing atmospheric CO2. Plant. Physiol. 44: 726–732.

    Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72: 248–254.

    Article  PubMed  CAS  Google Scholar 

  • Bürger J, Miyachi S, Galland P, Senger H (1988) Quantum requirements of photosynthetic oxygen evolution and 77 k fluorescence emission spectra in unicellular green algae grown under low-and high-CO2 conditions. Bot. Acta 101: 229–232.

    Google Scholar 

  • Cave G, Tolley LC, Strain BR (1981) Effects of carbon dioxide enrichment on chlorophyll content, starch and starch grain structure in Trifolium subteraneum. Physiol. Plant. 51: 171–174.

    Article  CAS  Google Scholar 

  • Corzo A, Niell FX (1991) Determination of nitrate reductase activity in Ulva rigida C. Agardh by the in situ method. J. exp. mar. Biol. Ecol. 146: 181–191.

    Article  CAS  Google Scholar 

  • Dubinsky Z, Falkowski G, Wyman K (1986) Light harvesting and utilization by phytoplankton. Plant Cell Physiol. 7: 1335–1349.

    Google Scholar 

  • Edwards G, Walker DA (1983) C3-C4 Cellular and Environmental Utilization by Phytoplankton. Blackwell, Oxford: 734 pp.

    Google Scholar 

  • Eley JH (1971) Effect of CO2 concentration on pigmentation in the blue-green alga Anacystis nidulans. Plant Cell Physiol. 12: 311–316.

    CAS  Google Scholar 

  • Figueroa FL, Aguilera J, Niell FX (1995) Red and blue light regulation of growth and photosynthetic metabolism in Porphyra umbilicalis (Bangiales, Rhodophyta). Eur. J. Phycol. 30: 11–18.

    Google Scholar 

  • García-Sánchez MJ, Fernández JA, Niell FX (1994) Effect of inorganic carbon supply on the photosynthetic physiology of Gracilaria tenuistipitata. Planta 194: 55–61.

    Article  Google Scholar 

  • Hanelt D, Wiencke C, Nultsh (1997) Influence of UV radiation on the photosynthesis of Arctic macroalgae in the field. Photochem. Photobiol. 38: 40–47.

    Article  CAS  Google Scholar 

  • Inskeep W, Bloom PR (1985). Extinction coefficients of Chlorophyll a and b in seawater. Limnol. Oceanogr. 27: 849–855.

    Google Scholar 

  • Johnston AM, Raven JA (1990) Effects of culture in high CO2 on the photosynthetic physiology of Fucus serratus. Br. phycol. J. 25: 75–82.

    Google Scholar 

  • Kroon B, Prézelin BB, Schofield O (1993) Chromatic regulation of quantum yields for photosystem II charge separation, oxygen evolution and carbon fixation in Heterocapsa pygmaea (Pyrrophyta). J. Phycol. 29: 453–462.

    Article  CAS  Google Scholar 

  • Lara C, Romero JM, Coronil T, Guerrero MG (1987) Interactions between photosynthetic nitrate assimilation and CO2 fixation in Cyanobacteria. In Ullrich WR, Aparicio JP, Syrett PJ, Castillo F (eds), Inorganic Nitrogen Metabolism, Springer-Verlag, Berlin: 45–60.

    Google Scholar 

  • Magnusson G, Larsson C, Axelsson L (1996) Effects of high CO2 treatment on nitrate and ammonium uptake by Ulva lactuca grown in different nutrient regimes. In Figueroa FL, Jiménez C, Pérez-Llorens JL, Niell FX (eds), Underwater Light and Algal Photobiology, Sci. Mar. 60 (Supl. 1): 179–189.

  • Mercado JM, Jiménez C, Niell FX, Figueroa FL (1996) Comparison of methods for measuring light absorption by algae and their application to the estimation of the package effect. In Figueroa FL, Jiménez C, Pérez-Llorens JL, Niell FX (eds), Underwater Light and Algal Photobiology, Sci. Mar. 60 (Suppl. 1): 39–45.

  • Mercado JM, Niell FX, Figueroa FL (1997) Regulation of the mechanism for HCO3- use by the inorganic carbon concentration in Porphyra leucosticta Thur. in Le Jolis (Rhodophyta). Planta 201: 319–325.

    Article  CAS  PubMed  Google Scholar 

  • Mercado JM, Viñegla B, Figueroa FL, Niell FX (1999) Isoenzymic forms of carbonic anhydrase in the red macroalga Porphyra leucosticta. Physiol. Plant. 106: 69–74.

    Article  CAS  Google Scholar 

  • Palmqvist K, Ramazanov Z, Samuelsson G (1990) The role of extracellular carbonic anhydrase for accumulation of inorganic carbon in the green alga Chlamydomonas reinhardtii. A comparison between wild type and cell-wall-less mutant cells. Physiol. Plant. 80: 267–276.

    Article  CAS  Google Scholar 

  • Petterson R, McDonald AJS (1992) Effects of elevated carbon dioxide concentration on photosynthesis and growth of small birch plants (Betula pendula Roth.) at optimal nutrition. Plant Cell Environ. 15: 911–919.

    Article  Google Scholar 

  • Poorter H, Gifford RM, Kriedemann PE, Wong SC (1992) A quantitative analysis of dark respiration and carbon content as factors in the growth response of plants to elevated CO2. Aust. J. Bot. 40: 501–513.

    Article  CAS  Google Scholar 

  • Ramazanov Z, Rawat M, Henk C, Mason C, Mathews S, Moroney J (1994) Correlation between the induction of the CO2 concentrating mechanism and pyrenoid starch sheath formation in Chlamydomonas reinhardtii. Planta 135: 210–216.

    Google Scholar 

  • Raven JA, Johnston AM (1991) Mechanisms of inorganic-carbon acquisition in marine phytoplankton and their implications for the use of other resources. Limnol. Oceanogr. 36: 1701–1714.

    Article  CAS  Google Scholar 

  • Raven JA, Lucas WJ (1985) The energetic of carbon acquisition. In Lucas WJ & Berry JA (eds), Inorganic Carbon Uptake by Aquatic Photosynthetic Organisms, American Society of Plant Physiologists, Rockville, Maryland: 305–324

    Google Scholar 

  • Riley JB, Chester R (1977) Introduction to Marine Chemistry. Academic Press, London and New York.

    Google Scholar 

  • Sage RF, Sharkey TD, Seemann JR (1989) Acclimation of photosynthesis to elevated CO2 in five C3 species. Plant Physiol. 89: 590–596.

    PubMed  CAS  Google Scholar 

  • Schreiber U, Schliwa U, Bilger W (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosyn. Res. 10: 51–62.

    Article  CAS  Google Scholar 

  • Sheen J (1990) Metabolic repression of transcription in higher plants. Plant Cell 2: 1027–1038.

    Article  PubMed  CAS  Google Scholar 

  • Sicher RC, Kremer DF, Rodermel SR (1994) Photosynthetic acclimation to elevated CO2 occurs in transformed tobacco with decreased ribulose-1,5-bisphosphate carboxylase/oxygenase content. Plant Physiol. 104: 409–415.

    PubMed  CAS  Google Scholar 

  • Sokal PR, Rohlf FJ (1981) Biometry: the principles and practice of statistics in biological research, 2nd edn. W. H. Freeman, San Francisco.

    Google Scholar 

  • Spalding MH, Critchley C, Govindjee C, Ogren WL (1984) Influence of carbon dioxide concentration during growth on fluorescence induction characteristics of the green alga Chlamydomonas reinhardtii. Photosyn. Res. 5: 169–176.

    Article  CAS  Google Scholar 

  • Spencer W, Bowes G (1986) Photosynthesis and growth of water hyacinth under CO2 enrichment. Plant Physiol. 82: 528–533.

    Article  PubMed  Google Scholar 

  • Starr R, Zeikus JA (1987) UTEX: the culture collection of algae at the University of Texas at Austin. J. Phycol. 23: 1–47.

    Google Scholar 

  • Stumm W, Morgan JJ (1981) Aquatic Chemistry, 2nd edn. W. H. Freeman and Co., San Francisco, California.

    Google Scholar 

  • Turpin DH (1991) Effects of inorganic N availability on algal photosynthesis and carbon metabolism. J. Phycol. 27: 14–20.

    Article  CAS  Google Scholar 

  • Van Oosten JJ, Afif D, Dizengremel P (1992) Long-term effects of a CO2 enriched atmosphere on enzymes of the primary carbon metabolism on spruce trees. Plant Physiol. Biochem. 30: 541–547.

    CAS  Google Scholar 

  • Vergara JJ, Bird KT, Niell FX (1995) Nitrogen assimilation following NH4+ pulses in the red alga Gracilariopsis lemaneiformis. Effect on C metabolism. Mar. Ecol. Progr. Ser. 122: 253–263.

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Departamento de Ecología, Facultad de Ciencias, Universidad de Málaga, Campus de Teatinos, E-29071, Málaga, Spain

    Jesús M. Mercado, F. Javier, L. Gordillo, F. Xavier Niell & Félix L. Figueroa

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  1. Jesús M. Mercado
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  2. F. Javier
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  3. L. Gordillo
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  4. F. Xavier Niell
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  5. Félix L. Figueroa
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Mercado, J.M., Javier, F., Gordillo, L. et al. Effects of different levels of CO2 on photosynthesis and cell components of the red alga Porphyra leucosticta . Journal of Applied Phycology 11, 455–461 (1999). https://doi.org/10.1023/A:1008194223558

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