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Mitochondrial DNA analysis of Sporothrix schenckii clinical isolates from China

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Abstract

Mitochondrial DNA (mtDNA) types based on restriction fragment length polymorphism (RFLP)patterns with HaeIII were investigated in clinical isolates of Sporothrix schenckii in China. In addition to 23 mtDNA types (Types 1–23) so far reported, a new mtDNA type (Type 24) was found in this study. Type 24 was divided into two subtypes, Subtype 24A and 24B based on RFLP with EcoRV. Sixty-seven isolates in China consisted of 58 isolates of Type 4, 5 of Type 6, 1 of Type 5, 1 of Type 20 and 2 of Type 24. Based on the phylogeny of the mtDNA types (Types 1–24) constructed by estimating sequence divergences of mtDNA, mtDNA types clustered into two groups: Group A (Types 1–3, Type 11, Types 14–19 and Types 22–23) and Group B (Types 4–10, Types 12–13,Types 20–21 and Type 24). These results suggest that mostS. schenckii isolates in China belong to Group B.

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Authors and Affiliations

  1. Department of Dermatology, Kanazawa Medical University, Uchinada, Ishikawa, 920-0293, Japan

    Junping Lin, Masako Kawasaki & Hiroshi Ishizaki

  2. School of Health Science, Faculty of Medicine, Kanazawa University, Ishikawa, 920-0934, Japan

    Masanori Aoki

  3. School of Health Science, Faculty of Medicine, Kanazawa University, Ishikawa, 920-0934, Japan

    Gang You

  4. Department of Dermatology, The First Hospital of Beijing Medical University, Beijing, 100034, China

    Ruoyu Li

  5. Department of Dermatology, First Hospital of China Medical University, Shenyang, Liaoning, 110001, China

    Junping Lin

Authors
  1. Junping Lin
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  2. Masako Kawasaki
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  3. Masanori Aoki
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  4. Hiroshi Ishizaki
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  5. Gang You
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  6. Ruoyu Li
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Lin, J., Kawasaki, M., Aoki, M. et al. Mitochondrial DNA analysis of Sporothrix schenckii clinical isolates from China. Mycopathologia 148, 69–72 (2000). https://doi.org/10.1023/A:1007147825316

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  • DOI: https://doi.org/10.1023/A:1007147825316

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