Photosynthetica 1999, 36(12):201-207 | DOI: 10.1023/A:1007099720226

Single-Turnover Flashes to Saturate the QA Reduction in a Leaf were Generated by the Light-Emitting Diodes from a Double Modulation Kinetic Chlorophyll Fluorometer

O. Urban1, M. Trtilek2, T. Feild3, L. Nedbal4
1 Laboratory of Ecological Physiology of Forest Trees, Institute of Landscape Ecology, Brno, Czech Republic
2 P.S. Instruments Ltd., Brno, Czech Republic
3 Department of Organismic & Evolutionary Biology, Harvard University, Cambridge, USA
4 NRC Photosynthesis and Global Climate Change, Institute of Microbiology, Academy of Sciences of the Czech Republic, Třeboň, Czech Republic

The earlier developed double-modulation chlorphyll (Chl) fluorometer was modified for measurements with intact leaves of higher plants. The Chl fluorometer is based on a non-periodic modulation of both actinic and measuring flashes. In addition, continuous orange actinic and far-red radiation were produced by separate arrays of light-emitting diodes (LEDs). Programmable timing of the flashes allows to cover a wide dynamic range from microseconds to minutes. We have demonstrated that the LEDs can produce single-turnover flashes that saturate QA reduction of intact leaves of Glyceria maxima and shoots of Picea abies.

Additional key words: chlorophyll fluorescence; Glyceria maxima; Picea abies; QA reduction

Published: September 1, 1999  Show citation

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Urban, O., Trtilek, M., Feild, T., & Nedbal, L. (1999). Single-Turnover Flashes to Saturate the QA Reduction in a Leaf were Generated by the Light-Emitting Diodes from a Double Modulation Kinetic Chlorophyll Fluorometer. Photosynthetica, 37(2), 201-207. doi: 10.1023/A:1007099720226
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    References

    1. Bolhàr-Nordenkampf, H.R., Öquist, G.: Chlorophyll fluorescence as a tool in photosynthesis research.-In: Hall, D.O., Scurlock, J.M.O., Bolhàr-Nordenkampf, H.R., Leegood, R.C., Long, S.P. (ed.). Photosynthesis and Production in a Changing Environment: A Field and Laboratory Manual. Pp. 193-206. Chapman and Hall, London-Glasgow-New York-Tokyo-Melbounre-Madras 1993. Go to original source...
    2. Evans, J.R., Jakobsen, I., Ögren, E.: Photosynthetic light-response curves. 2. Gradients of light absorption and photosynthetic capacity.-Planta 189: 191-200, 1993. Go to original source...
    3. Field, T.S., Nedbal, L., Ort, D.R.: Nonphotochemical reduction of the plastoquinone pool in sunflower leaves originates from chlororespiration.-Plant Physiol. 116: 1209-1218, 1998. Go to original source...
    4. Lavergne, J., Leci, E.: Properties of inactive Photosystem II centers.-Photosynth. Res. 35: 323-343, 1993. Go to original source...
    5. Marek, M.V., Kalina, J., Nauš, J.: Effect of thinning on parameters of photosynthetic characteristics of Norway spruce canopy. II. Seasonal changes of photosynthetic pigment content.-Lesnictví-Forestry 43: 206-214, 1997.
    6. Nedbal, L., Trtílek, M.: Diffuse-light dual modulation fluorometer: monitoring of electron transfer reactions in Synechococcus elongatus exposed to intermittent light.-In: Mathis, P. (ed.) Photosynthesis: From Light to the Biosphere. Vol. V. Pp. 813-816. Kluwer Academic Publ., Dordrecht-Boston-London 1995. Go to original source...
    7. Nedbal, L., Trtílek, M., Kaftan, D.: Flash fluorescence induction: A novel method to study regulation of photosystem II.-J. Photochem. Photobiol. B 48: 154-157, 1999. Go to original source...
    8. Šesták, Z.: Determination of chlorophylls a and b.-In: Šesták, Z., Čatský, J., Jarvis, P.G. (ed.): Plant Photosynthetic Production. Manual of Methods. Pp. 672-701. Dr W. Junk N.V. Publ., The Hague 1971.
    9. Trtílek, M., Kramer, D.M., Koblížek, M., Nedbal, L.: Dual-modulation LED kinetic fluorometer.-J. Lumin. 72-74: 597-599, 1997. Go to original source...

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