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The Local Effect of Paf on Leukocyte Adherence to Small Bowel Mesenteric Venules Following Intra-abdominal Contamination

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Abstract

We have previously demonstrated that intra-abdominal contamination increases neutrophil infiltration into the gastrointestinal tract. The purpose of our current study was twofold: 1) to determine if leukocyte adherence to the mesenteric microvasculature occurred by local peritoneal contamination or by systemic mechanisms; and 2) to assess the role of platelet activation factor (PAF) in this process. Rats underwent cecal ligation and puncture (CLP), and 4 h after the procedure we used intravital microscopy to visualize the mesenteric microcirculation. Cecal ligation and puncture increased leukocyte adherence (22.3 ± 5.5 leukocytes/100 μm) vs. sham (2.3 ± 0.9, P < 0.05). WEB-2086, a PAF receptor antagonist, prevented this increase (6.47 ± 4.8, P < 0.05). To assess if leukocyte adherence was due to topical effects, we performed similar experiments with the small bowel exteriorized. In such cases, CLP did not increase leukocyte adherence (1.2 ± 0.8 vs. 1.4 ± 0.9). In addition, topical application of highly diluted fecal matter (1 : 1000) increased leukocyte adherence (4.8 ± 1.2) vs. control (0.6 ± 0.3, P < 0.05). Our study demonstrates that leukocyte adherence in the mesenteric microcirculation following intra-abdominal contamination is due to direct topical exposure to fecal matter, and it is mediated by PAF.

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Authors and Affiliations

  1. Department of Surgery, University of Kansas Medical Center, Kansas City, Kansas, 66160

    David M. Smalley, Ed W. Childs & Laurence Y. Cheung

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  1. David M. Smalley
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  2. Ed W. Childs
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  3. Laurence Y. Cheung
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Smalley, D.M., Childs, E.W. & Cheung, L.Y. The Local Effect of Paf on Leukocyte Adherence to Small Bowel Mesenteric Venules Following Intra-abdominal Contamination. Inflammation 24, 399–410 (2000). https://doi.org/10.1023/A:1007008027483

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