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The Influence of Retinoic Acid and Retinoic Acid Derivatives on β2 Integrins and L-Selectin Expression in HL-60 Cells In Vitro

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Abstract

A decreased expression of the β2-integrin CD11b molecules on peripheral neutrophils from patients with pustular psoriasis occurred during treatment with retinoid compounds. Since this effect could not be mimicked in vitro with isolated peripheral neutrophils, the effect of retinoid compounds on cell differentiation was investigated. The promyelocytic cell line, HL60, was used to study what effect different retinoid compounds had on the cell surface expression of CD11b and L-selectin (CD62L) molecules, complement-mediated phagocytosis, adhesion and the oxidative burst. Retinoid-differentiated cells showed a significantly lower expression of CD11b and CD62L, and a decreased phagocytosis and oxidative burst compared to DMSO-differentiated HL60 cells or peripheral blood neutrophils. The diminished expression of β2-integrins or L-selectin did not affect their adhesion to non-activated or lipopolysaccharide-activated endothelial cells in vitro but may however affect adhesion to vascular endothelium under shear forces during blood flow. These results suggest that retinoid treatment could affect several early steps in the inflammatory process.

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Authors and Affiliations

  1. Department of Biomedicine and Surgery, Division for Dermatology, Sweden

    Florence Sjögren

  2. Department of Health and Environment, Division for Medical Microbiology, Clinical Research Center, Faculty of Health Sciences, Linköping University, Linköping, Sweden

    Olle Stendahl

  3. Department of Clinical Immunology, Karolinska Hospital, Stockholm, Sweden

    Olof Ljunghusen

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  1. Florence Sjögren
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  2. Olle Stendahl
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  3. Olof Ljunghusen
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Sjögren, F., Stendahl, O. & Ljunghusen, O. The Influence of Retinoic Acid and Retinoic Acid Derivatives on β2 Integrins and L-Selectin Expression in HL-60 Cells In Vitro. Inflammation 24, 21–32 (2000). https://doi.org/10.1023/A:1006983824890

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