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TNF-α induced altered signaling mechanism in human neutrophil

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Abstract

In the present study we investigated the TNF-α induced signal transduction mechanism in human neutrophil. Exogenously added TNF-α affects both PKC activity and its translocation from cytosol to the membrane. Endogenous protein phosphorylation pattern is inhibited in TNF-α induced neutrophil in Ca-dependent and Ca-independent manner, including a major 47 and 66 kDa cytosolic proteins, which may be implicated in superoxide anion generation. However TNF-α dose dependently enhances the expression of ζ-PKC isotype but not the β-PKC. Morphology and cell cytotoxicity are studied in TNF-α treated neutrophil to understand the TNF-α induced cell death or apoptosis and these experiment is further confirmed by DNA fragmentation analysis. These results clearly demonstrate that TNF-α induces cellular death of human neutrophil at least in part by enhanced expression of Ca-independent ζ-PKC. These observations provide an insight towards understanding the function of ζ-PKC in apoptotic pathway.

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Authors and Affiliations

  1. Department of Microbiology, Bose Institute, P 1/12 C.I.T. Scheme VII M, Calcutta, India

    Sonali Das, Sandip Bhattacharyya, Sanjukta Ghosh & Subrata Majumdar

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  1. Sonali Das
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  2. Sandip Bhattacharyya
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  3. Sanjukta Ghosh
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  4. Subrata Majumdar
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Das, S., Bhattacharyya, S., Ghosh, S. et al. TNF-α induced altered signaling mechanism in human neutrophil. Mol Cell Biochem 197, 97–108 (1999). https://doi.org/10.1023/A:1006935114624

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