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Expression of the nuclear gene encoding mitochondrial ATP synthase subunit α in early development of Drosophila and sea urchin

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Abstract

Complementary DNAs encoding nuclear-coded mitochondrial ATP synthase subunit α of Drosophila melanogaster and Strongylocentrotus purpuratus were obtained by a combination of library screening and redundant PCR. The entire coding sequence of the precursor polypeptide was inferred for both species. Southern blots to genomic DNA indicated that the gene is almost certainly single-copy in both organisms. Northern blots to RNA from staged developmental series showed that ATP synthase subunit α mRNA is represented in the egg, declines in abundance during cleavage, and is replenished by zygotic transcription in both species. However, the extent and timing of these changes differ significantly in the two species studied. Nuclear-coded and mitochondrially encoded ATP synthase genes appear to be temporally co-regulated in Drosophila, but not sea urchin development.

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Authors and Affiliations

  1. Departamento de Bioquímica, UAM/Instituto de Investigaciones Biomédicas CSIC, Arzobispo Morcillo 4, 28029, Madrid, Spain

    Ana Talamillo & Rafael Garesse

  2. Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, 56 Dumbarton Rd, Glasgow, G11 6NU, Scotland, UK

    Alexander A. K. Chisholm

  3. Institute of Medical Technology, University of Tampere, PO Box 607, 33101, Tampere, Finland

    Howard T. Jacobs

Authors
  1. Ana Talamillo
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  2. Alexander A. K. Chisholm
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  3. Rafael Garesse
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  4. Howard T. Jacobs
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Talamillo, A., Chisholm, A.A.K., Garesse, R. et al. Expression of the nuclear gene encoding mitochondrial ATP synthase subunit α in early development of Drosophila and sea urchin. Mol Biol Rep 25, 87–94 (1998). https://doi.org/10.1023/A:1006868306735

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