Skip to main content
SpringerLink
Log in

Rollinia mucosa cell suspension cultures: Establishment and growth conditions

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Different types and concentrations of plant growth regulators were tested in order to obtain the best callus and cell suspension culture growth conditions of Rollinia mucosa (Jacq.) Baill. (Annonaceae). Picloram was shown to be the most efficient for induction and production of friable calluses, independent of the concentration used. Cellular morphology and viability, fresh and dry weights, pH and medium sugar concentration were determined for cell suspension cultures. Dissimilation curves were used for the characterization of the growth of cell suspension cultures. Picloram provided the most rapid growth and produced the highest biomass, with little variation in morphology (differentiated cells). It also provided the highest dissimilation, when compared with cell suspension cultures maintained in media with 2,4-D or NAA + BA + GA3. Stable cell suspension cultures can be established in MS medium supplemented with 20.8 μM picloram.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bongaerts RJM (1998) The chorismate branching point in Catharanthus roseus. Aspects of anthranilate synthase regulation in relation to indole alkaloid biosynthesis. Ph.D. Thesis, University of Leiden, The Netherlands (160 p)

    Google Scholar 

  • Caetano LC & Dadoun H (1987) Pallidine and aporphinoid alkaloids from Rollinia mucosa. J. Nat. Prod. 50: 330

    Article  CAS  Google Scholar 

  • Campos LMM, Nunan EA, Caetano LC, Dadoun H & Oliveira AB (1985) Atividade antimicrobiana de extratos de Rollinia mucosa (Baill.) (Annonaceae). Abstract 37a Reunião Anual SBPC (p. 815)

  • Castro-Faria-Neto HC, AraÚjo CV, Moreira S, Bozza PT, Thomas G, Barbosa-Filho JM, Cordeiro RSB & Tibiriçá EV (1995) Yangambin: A new naturally-occurring platelet-activating factor receptor antagonist: in vivo pharmacological studies. Plant Med. 61: 106–112

    CAS  Google Scholar 

  • Chaplin MF & Kennedy JFC (1986) Carbohydrate analysis; a practical approach. IRL Press (p 3)

  • Chávez D, Acevedo LA & Mata R (1998) Jimenezin, a novel Annonaceous acetogenin from seeds of Rollinia mucosa containing adjacent tetrahydrofuran ring systems. J. Nat. Prod. 61: 419–421

    Article  Google Scholar 

  • Chávez D, Acevedo LA & Mata R (1999) Tryptamine derived amides and acetogenins from seeds of Rollinia mucosa. J. Nat. Prod. 62(8) 1119–1122

    Article  PubMed  CAS  Google Scholar 

  • Chen YY, Chang FR, Yen HF & Wu YC (1996) Epomusenins A and B, two acetogenins from fruits of Rollinia mucosa. Phytochemistry 42: 1081–1083

    Article  CAS  Google Scholar 

  • Chi G, Barfield DG, Sim G & Pua E (1990) Effect of AgNO3 and amino-ethoxyvinylglycine on in vitro shoot and root organogenesis from seedling explants of recalcitrant Brassica genotypes. Plant Cell Rep. 9: 195–198

    Article  CAS  Google Scholar 

  • Figueiredo SFL (1996) Desenvolvimento de um sistema de cultura in vitro para a produção de lignanas furofurânicas a partir de Rollinia mucosa (Jacq.) Baill. Ph.D. Thesis, Universidade Federal do Rio de Janeiro, Rio de Janeiro (136 p)

    Google Scholar 

  • Figueiredo SFL, Viana VRC, Simões C, Albarello N, Trugo LC, Kaplan MAC & Krul WR (1999) Lignans from leaves, seedlings and micropropagated plants of Rollinia mucosa (Jacq.) Baill. – Annonaceae. Plant Cell Tiss. Org. Cult. 56: 121–124

    Article  CAS  Google Scholar 

  • Fontana JD, Almeida ERA, Baron M, Guimarães MF, Deschamps FC, Schwartsmann G, Cervi AC, Zanette F, Vilegas J & Lanças FM (1994) Screening of acetogenin-producing plants in Brazilian flora – Annona and Rollinia seed oil as a source of the bioactive compounds and subproducts. Appl. Biochem. Biotech. 45/46: 295–313

    Article  Google Scholar 

  • Furmanowa M, Glowniak K, Syklowska-Baranek K, Zgórka G & Józefczyk A (1997) Effect of picloram and methyl jasmonate on growth and taxane accumulation in callus culture of Taxus_media var. Hatfieldii. Plant Cell Tiss. Org. Cult. 49: 75–79

    Article  CAS  Google Scholar 

  • Furmanowa M, Hartwich M, Alfermann AW, Kozminski W & Olejnik M(1999) Rosavin as a product of glycosylation by Rhodiola rosea (roseroot) cell cultures. Plant Cell Tiss. Org. Cult. 56: 105–110

    Article  CAS  Google Scholar 

  • Geerlings A (1999) Strictosidine β-D glucosidase an enzyme in the biosynthesis of pharmaceutically important indole alkaloids. Ph.D. Thesis, University of Leiden, The Netherlands (117 p)

    Google Scholar 

  • Gu ZM, Zhou DW, Lewis NJ, Wu J, Shi GE & McLaughlin JL (1997) Isolation of new bioactive annonaceous acetogenins from Rollinia mucosa guided by liquid chromatography mass spectrometry. Bioorgan. Med. Chem. 5(10) 1911–1916

    Article  CAS  Google Scholar 

  • Hagendoorn MJM, van der Plas LHW & Segers GJ (1994) Accumulation of anthraquinones in Morinda citrifolia cell suspensions. Plant Cell Tiss. Org. Cult. 38: 227–234

    Article  CAS  Google Scholar 

  • Halaweish FT & Tallamy DW (1998) Production of cucurbitacins by cucurbit cell cultures. Plant Sci. 131: 209–218

    Article  CAS  Google Scholar 

  • Hallard D, van der Heijden R, Contin A, Tomás-Jimenes EM, Snoeijer W, Verpoorte R, Jensen SR, Lopes-Cardoso MI, Pasquali G, Memelink J & Hoge HC (1998) An assay for secologanin in plant tissues based on enzymatic conversion into strictosidine. Phytochem. Anal. 9: 162–167

    Article  CAS  Google Scholar 

  • Jordan M, Arce P & Roveraro C (1990) Micropropagation in vitro de algunas especies frutícolas de Chile. Cienc. Invest. Agraria 17 (3) 111–117

    Google Scholar 

  • Ketchu, REB, Gibson DM & Gallo LG (1995) Media optimization for maximum biomass production in cell cultures of pacific yew. Plant Cell Tiss. Org. Cult. 42: 185–193

    Article  Google Scholar 

  • Langezaal CR & Scheffer JJC (1992) Initiation and growth characterization of some hop cell suspension cultures. Plant Cell Tiss. Org. Cult. 30: 159–164

    Article  CAS  Google Scholar 

  • Larkin P (1976) Purification and viability determinations of plant protoplasts. Planta 128: 213–216

    Article  Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and biossays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Article  CAS  Google Scholar 

  • Nair S, Gupta PK, Shirgurkar MV & Mascarenhas AF (1984) In vitro organogenis from leaf explants of Annona squamosa Linn. Plant Cell Tiss. Org. Cult. 3: 29–40

    Article  Google Scholar 

  • Pan JX, Henses OD, Zink DL, Chang MN & Hwang SB (1987) Lignans with platelet activating factor antagonist activity from Magnolia biondii. Phytochemistry 26: 1377–1379

    Article  CAS  Google Scholar 

  • Paulo MQ, Kaplan MAC, Laprévote O, Roblot F, Hocque-Miller R & Cavé A (1991) Lignans and other non-alkaloidal constituents from Rollinia mucosa, Annonaceae. Fitoterapia LXII (2): 150–152

    Google Scholar 

  • Paulo MQ (1992) Estudo químico-ecológico de Annonaceae. Ph.D. Thesis, NÚcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, Rio de Janeiro (152 p)

    Google Scholar 

  • Peltonen S, Mannonen L & Karjalainen R (1997) Elicitor-induced changes of phenylalanine ammonia-lyase activity in barley cell suspension cultures. Plant Cell Tiss. Org. Cult. 50: 185–193

    Article  CAS  Google Scholar 

  • Ryu DDY, Lee SO & Romani RJ (1990) Determination of growth rate for plant cell cultures: comparative studies. Biotech. Bioeng. 35: 305–311

    Article  CAS  Google Scholar 

  • Schripsema J, Meijer AH, Van Iren F, Ten Hoopen HJG & Verpoorte R (1990) Dissimilation curves as a simple method for the characterization of growth of plant cell suspension cultures. Plant Cell Tiss. Org. Cult. 22: 55–64

    Google Scholar 

  • Shi G, Macdougal JM & Mclaughlin JL (1997) Bioactive annonaceous acetogenins from Rollinia mucosa. Phytochemistry 45: 719–723

    Article  PubMed  CAS  Google Scholar 

  • Sierra MI (1991) Aspects of indole alkaloid accumulation in Tabernaemontana tissue cultures: differentiation, peroxidases and stability. Ph.D. Thesis, University of Leiden, The Netherlands (132 p)

    Google Scholar 

  • Szabados L, Mroginski LA & Roca WM (1991) Suspensiones celulares: descripción, manipulación y aplicaciones. In: Roca WM & Mroginski LA (eds.). Cultivo de Tejidos en la Agricultura: Fundamentos y Aplicaciones (pp. 173–210). Centro Internacional de Agricultura Tropical, Colômbia

    Google Scholar 

  • Wang HQ, Yu JT & Zhong JJ (2000) Significant improvement of taxane production in suspension cultures of Taxus chinensis by sucrose feeding strategy. Process Biochem. 35(5): 479–483

    Article  CAS  Google Scholar 

  • Whitmer S (1999) Aspects of terpenoid indole alkaloid formation by transgenic cell line of Catharanthus roseus overexpressing tryptophan decarboxilase and strictosidine synthase. Ph.D. Thesis, University of Leiden, The Netherlands (228 p)

    Google Scholar 

  • Williams J, Pink DAC & Biddington NL (1990) Effect of silver nitrate on long-term culture and regeneration of callus from Brassica oleracea var. gemmifera. Plant Cell Tiss. Org. Cult. 21: 61–66

    Article  CAS  Google Scholar 

  • Zhong JJ, Chen F & Hu WW (2000) High density cultivation of Panax notoginseng cells in stirred bioreactors for the production of ginseng biomass and ginseng saponin. Process Biochem. 35: 491–496

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. Cláudia Simões & Vera Regina Campos Viana

    Present address: , Avenida Epitácio Pessoa, 2900/204, Lagoa, Rio de Janeiro, Brazil, CEP-22471-000

Authors and Affiliations

  1. Laboratório de Biotecnologia de Plantas (LABPLAN), Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Rua São Francisco Xavier, 524, PHLC, s/ 509, Maracanã, RJ, Brasil, CEP-20550-013

    Solange Faria Lua Figueiredo, Solange Faria Lua Figueiredo, Cláudia Simões, Norma Albarello & Vera Regina Campos Viana

Authors
  1. Solange Faria Lua Figueiredo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Cláudia Simões
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Norma Albarello
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Vera Regina Campos Viana
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Solange Faria Lua Figueiredo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Figueiredo, S.F.L., Simões, C., Albarello, N. et al. Rollinia mucosa cell suspension cultures: Establishment and growth conditions. Plant Cell, Tissue and Organ Culture 63, 85–92 (2000). https://doi.org/10.1023/A:1006483822768

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006483822768

Search

Navigation