Abstract
An improved technique for long-term culture of rice caryopses is necessary for physiological and genetic studies. Panicles of three rice (Oryza sativa) cultivars `Lemont', `Gummo-byeu' and `Hwasung-byeu' were cultured in liquid media with combinations of light versus dark, panicle position, nitrogen level (5-40 mM), and sucrose level (29–351 mM). Grain growth was increased when panicles were positioned horizontally, partially submerged in the media, owing to greater media contact and apoplastic uptake as observed by fluorescent dyes. The optimal media assimilate supply included 175 mM sucrose and 5 mM nitrogen. Grain fill occurred for up to four weeks; grain dry weight reached 80% of that on intact plants, with 50% germination. This technique should allow for future physiological studies with rice or other panicle-bearing species.
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References
Coffman WR& Juliano BO (1987) Rice. In: Nutritional Quality of Cereal Grains: Genetic and Agronomic Improvement-Agronomy Monograph no. 28 (pp 101–131). ASA-CSSA-SSSA, Madison,WI
Czyzewicz JR& Below FE (1994) Genotypic variation for nitrogen uptake by maize kernels grown in vitro. Crop Sci. 34: 1003–1008
Faleiros RRS, Seebauer JR& Below FE (1996) Nutritionally induced changes in endosperm of shrunken-1 and brittle-2 maize kernels grown in vitro. Crop Sci. 36: 947–954
Felker FC (1992) Participation of cob tissue in the uptake of medium components by maize kernels cultured in vitro. J. Plant Physiol. 139: 647–652
Gomez KA (1979) Effect of environment on protein and amylose content of rice. In: Chemical Aspects of Rice Grain Quality (pp 59–68). IRRI, Los Banos, Philippines
Hanft JM& Jones RJ (1986) Kernel abortion in maize I. Carbohydrate concentration patterns and acid invertase activity of maize kernels induced to abort in vitro. Plant Physiol. 81: 503–510
Heberer JA& Below FE (1989) Mixed nitrogen nutrition and productivity of wheat grown in hydroponics. Ann. Bot. 63: 643–649
Khanna HK& Raina SK (1998) Genotype X culture media interaction effects on regeneration response of three Indica rice cultivars. Plant Cell Tiss. Org. Cult. 52: 145–153
Kirsten WJ (1983) Rapid, automatic, high capacity Dumas determination of nitrogen. Microchemical J. 28: 529–547
Ma Y-Z, MacKown CT& van Sanford DA (1996) Kernel growth of in vitro cultured wheat spikes of cultivars with divergent sourcesink limitations. Plant Sci. 119: 135–148
Singh BK& Jenner CF (1983) Culture of detached ears of wheat in liquid culture: Modification and extension of the method. Aust. J. Plant Physiol. 10: 227–236
Singletary GW& Below FE (1989) Growth and composition of maize kernels cultured in vitro with varying supplies of carbon and nitrogen. Plant Physiol. 89: 341–346
Villareal RM& Juliano BO (1987) Effect of sucrose level on rice grain deposition in detached panicle system. Phytochem. 26: 3121–3125
Wang N& Fisher DB (1994) The use of fluorescent tracers to characterize the post-phloem transport pathway in maternal tissues of developing wheat grains. Plant Physiol. 104: 17–27
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Lee, H., Seebauer, J. & Below, F. An improved technique for culture of rice panicles. Plant Cell, Tissue and Organ Culture 60, 55–60 (2000). https://doi.org/10.1023/A:1006477021890
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DOI: https://doi.org/10.1023/A:1006477021890