Abstract
The effects of UVB on the kinetics of stem elongation of wild type (WT) and photomorphogenic mutants of tomato were studied by using linear voltage transducers connected to a computer. Twenty-one or twenty-six-day-old plants, grown in 12 h white light (150 μmol m−2 s−1 PAR)/12 h dark cycles, were first transferred to 200 μmol m−2 s−1 monochromatic yellow light for 12 h, then irradiated with 0.1 or 4.5 μmol m−2 s−1 UVB for 12 h and finally kept in darkness for another 24 h. The measurements of the kinetics of stem elongation started after 4 h under yellow light. Significant differences in stem growth during the irradiation with yellow light, as well as during the dark period, were found between the genotypes. In darkness, the magnitude of stem growth followed the order: tri > AC = fri > MMau > hp1. Two factors determined the large differences of growth in darkness: 1) the different stem elongation rate (SER) and 2) the different duration of the growing phase among the genotypes. In darkness the stem growth of au and hp1 mutants lasted for about 18 h, whereas it continued for the whole experimental period (36 h) in the other genotypes. UVB irradiation substantially reduced elongation growth of all genotypes (4.5 μmol m−2 s−1 being more effective than 0.1 μmol m−2 s−1). Both fluence rates of UVB induced a detectable reduction of SER already after 15 min of irradiation. Red light inhibited, while far red light promoted stem growth of all the genotypes tested. fri (phyA null), tri (phyB1 null), hp1 (exhibiting exaggerated phytochrome responses) mutants and WT tomato showed similar levels of UVB–induced inhibition of growth, while the aurea mutant showed the largest growth inhibition during the 12 h of irradiation. These results indicate that phytochrome is not directly involved in UVB control of stem elongation. The results of dichromatic irradiations UVB + red or UVB + far red indicate the presence of distinct and additive action of UVB photoreceptor and of the phytochrome system in the photoregulation of stem growth.
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References
Ahmad M, Jarillo J and Cashmore AR (1998) Chimeric proteins between cry1 and cry2 Arabidopsis blue light photoreceptors indicate overlapping functions and varying protein stability. Plant Cell10: 197–207
Ballaré CL, Barnes PW and Kendrick RE (1991) Photomorphogenic effects of UV-B radiation on hypocotyl elongation in wild type and stable-phytochrome-deficient mutant seedlings of cucumber. Physiol Plant 83: 652–658
Ballaré CL, Barnes PW and Flint S (1995) Inhibition of hypocotyl elongation by ultraviolet-B radiation in de-etiolating tomato seedlings. I. The photoreceptor. Physiol Plant 93: 584–592
Beggs CJ, Holmes MG, Jabben M and Schaefer E (1980) Action spectra for the inhibition of hypocotyl growth by continuous irradiation in light and dark-grown Sinapis alba L. seedlings. Plant Physiol 66: 615–618
Bertram L and Lercari B (1997) Kinetics of stem elongation in lightgrown tomato plants. Responses to different photosynthetically active radiation levels by wild-type and aurea mutant plants. Photochem Photobiol 66: 396–403
Cashmore AR, Jarillo JA, Wu Yj and Liu D (1999) Chryptochromes; blue light receptors of plants and animals. Science 284: 760–765
Christie JM and Jenkins GI (1996) Distinct UV-B and UV-A/ blue light signal transduction pathways induce chalcone synthase gene expression in Arabidopsis cells. Plant Cell 8: 1555–1567
Deckmin G, Martens C and Impens I (1994) The importance of the ratio UV-B/photosynthetic active radiation (PAR) during leaf development as determining factor of plant sensitivity to increased UV-B irradiance: Effects on growth, gas eschange and pigmentation of bean plants (Phaseolus vulgaris cv Label). Plant Cell Environ 17: 295–301
Frohnmeyer H, Bowler C, Zhu J-K, Yamagata H, Schaefer E and Chua N h (1998) Different roles for calcium and calmodulin in phytochrome-and UV-regulated expression of chalcone synthase. Plant J 13: 763–772
Fuglevand G, Jackson JA and Jenkins GI (1996) UV-B, UV-A and blue light signal transduction pathways interact synergistically to regulate chalcone synthase gene expression in Arabidopsis. Plant Cell 8: 2347–2357
Goto N, Yamamoto KT and Watanabe M (1993) Action spectra for inhibition of hypocotyl growth of wild-type plants and of the hy2 long hypocotyl mutant of Arabidopsis thaliana L. Photochem Photobiol 57: 867–871
Hartmann KM (1966) A general hypothesis to interpret 'high energy phenomena' of photomorphogenesis on the basis of phytochrome. Photochem Photobiol 5: 349–366
Hauser BA, Cordonnier-Pratt MM and Pratt LH (1998) Temporal and photoregulated expression of five tomato phytochrome genes. Plant J 14: 431–439
Heim B and Schaefer H (1982) Light-controlled inhibition of hypocotyl growth in Sinapis alba seedlings. Fluence rate dependency of hourly light pulses and continuous irradiation. Planta 154: 150–155
Hennig L, Poppe C, Unger S and Schafer E ( 1999) Control of hypocotyl elongation in Arabidopsis thaliana by photoreceptor interaction. Planta 208: 257–263
Kendrick RE, Kerckhoffs LHJ, Van Tuinen A and Koornneef m (1997) Photomorphogenic mutants of tomato. Plant Cell Environ 20: 746–751
Kerckhoffs LHJ, Sengers MMT and Kendrick RE (1997) Growth analysis of wild-type and photomorphogenic-mutant tomato plants. Physiol Plant 99: 309–315
Kim BC, Tennessen DJ and Last RL (1998) UV-B-induced photomorphogenesis in Arabidopsis thaliana. Plant J 15: 667–674
Jansen MAK, Gaba V and Greenberg BM(1998) Higher plants and UV-B radiation: Balancing damages, repair and acclimation. Trends Plant Sci 3: 131–135
Lercari B (1991) Photomorphogenic responses to UV light: Involvement of phytochrome and UV photoreceptors. In: Pottier RH, Douglas RH, Mathis P and Valenzeno DP (eds) Photobiological Techniques, pp 231–248. Plenum Press, New York/London
Lercari B and Sodi F (1992) Photomorphogenic responses to UV radiation II: A comparative study of UV effects on hypocotyl elongation in a wild type and an aurea mutant of tomato (Lycopersicon esculentum Mill.). Photochem Photobiol 56: 651–654
Lercari B, Sodi F and Lipucci di Paola M (1990) Photomorphogenic responses to UVB radiation: Involvement of phytochrome and UVB photoreceptors in the control of hypocotyl elongation in Lycopersicon esculentum Physiol Plant 79: 668–672
Lercari B, Moscatelli S, Ghirardi E, Niceforo R and Bertram L (1999) Photomorphogenic control of shoot regeneration from etiolated and light-grown hypocotyls of tomato. Plant Sci 140: 53–62
Lin C, Yang H, Guo H, Mockler T, Chen J and Cashmore AR (1998) Enhancement of the blue-light sensitivity of Arabidopsis seedlings by a blue light receptor cryptochrome 2. Proc Natl Acad Sci USA 95: 2686–2690
Lipucci di Paola M, Collina Grenci F, Caltavuturo L, Tognoni F and Lercari B 1988 A phytochrome mutant from tissue culture of tomato. Adv Hort Sci 2: 30–32
Liscum E and Briggs WR (1995) Mutations in the nph1 locus of Arabidopsis disrupt the perception of phototropic stimuli. Plant Cell 7: 473–485
Ninu L, Ahmad M, Miarelli C, Cashmore AR and Giuliano G (1999) Chryptochrome 1 controls tomato development in response to blue light. Plant J 18: 551–556
Perrotta G, Ninu L, Flamma F, Weller J, Kendrick RE, Nebuloso E and Giuliano G (1999) The chryptochrome gene family of tomato. In: Book of Abstracts, p. 39, European Symposium on Photomorphogenesis, March 21-26, 1999, Berlin
Peters JL, Schreuder MEL, Verduin SJE and Kendrick RE (1992) Physiological characterisation of a high-pigment mutant of tomato. Photochem Photobiol 56: 75–82
Pratt LH and Briggs WR (1966) Photochemical and non photochemical reactions of phytochrome in vivo. Plant Physiol 41: 467–474
Quail PH, Boylan MT, Parks BM, Short TW, Xu Y and Wagner D (1995) Phytochromes: Photosensory perception and signal transduction. Science 268: 675–680
Stapleton AE (1992) Ultraviolet radiation and plants: Burning questions. Plant Cell 4: 1353–1358
Terry MJ and Kendrick RE (1996) The aurea and yellow-green-2 mutants of tomato are deficient in phytochrome chromophore synthesis. J Biol Chem 271: 21681–21686
Tevini M and Teramura AH (1989) UV-B effects on terrestrial plants. Photochem Photobiol 50: 479–487
Van Tuinen A, Kerckhoffs LHJ, Nagatani A, Kendrik RE and Koornneef M (1995a) Far-red light insensitive, phytochrome A-deficient mutants of tomato. Mol Gen Genet 246: 133–141
Van Tuinen A, Kerckhoffs LHJ, Nagatani A, Kendrik RE and Koornneef M (1995b) A temporarily red-light insensitive mutant of tomato lacks a light-stable, B-like phytochrome. Plant Physiol 108: 939–947
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Bertram, L., Lercari, B. Evidence against the involvement of phytochrome in UVB-induced inhibition of stem growth in green tomato plants. Photosynthesis Research 64, 107–117 (2000). https://doi.org/10.1023/A:1006459316266
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DOI: https://doi.org/10.1023/A:1006459316266