Skip to main content
SpringerLink
Log in

Genotypic variation in anther culture and effect of ovary coculture in durum wheat

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

The response of anthers to in vitro culture and the effect of coculture of ovaries on anther culturability have been studied in responsive and recalcitrant cultivars of durum wheat (Triticum turgidum ssp. durum) from Morocco and ICARDA. A large genotypic-dependence of anther culture has been shown in 18 cultivars. Their response in term of callus and embryo induction varied from 0 to 13%. Coculture of ovaries with anthers enhanced the response of the most responsive genotype (cv. Sarif) and removed the recalcitrance in Cocorit and Isly cultivars. However, there was no effect of anther-ovary coculture on green plant regeneration. The implication of the genome and the media conditioning by the ovaries on anther response is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alejar MS, Zapata FJ, Senadhira D, Khush GS & Datta SK (1995) Utilization of anther culture as a breeding tool in rice improvement. In: Terzi, M. et al., (eds) Current Issues in Plant Molecular and Cellular Biology (pp 137–142). Kluwer Academic Publishers, Dordrecht

    Google Scholar 

  • Autrique E, Nachit M, Monneveux P, Tanksley S & Sorrells M (1996) Genetic diversity in durum wheat based on RFLP's, morphological traits and coefficient of parentage. Crop Sci. 36: 735–742

    Article  Google Scholar 

  • Benlhabib O & Nabors MW (1996) Evaluation of embryogenic and androgenic potentials in tissue culture of Moroccan barley. Rachis 15: 15–20

    Google Scholar 

  • Catteno M, Qiao YM & Pogna NE (1991) Embryoid induction and green plant regeneration from cultured anthers in durum wheat line homozygous for the 1BL/1RS translocation. J. Genet. Br. 45: 369–372

    Google Scholar 

  • Darvey N (1998) Doubled haploid technology: an alternative model for germoplasm enhancement. Int. Wheat Genet. Sym. 9: 148–151

    Google Scholar 

  • Datta S & Wenzel G (1987) Isolated microspore derived plant formation via embryogenesis in Triticum aestivum L. Plant Sci. 48: 49–54

    Article  Google Scholar 

  • De Buyser J & Henry Y (1996) Analyse génétique de l'aptitude à l'androgenèse chez le blé tendre. In: Petitprez (ed) Biotechnologie Végétales "Haplodiploidisation" (pp 98–101). Centre National d'Enseignement à Distance, Institut de Rennes, Toulouse.

    Google Scholar 

  • De Buyser J & Picard E (1997) Obtention de plantes chlorophylliennes haploïdes de blé dur et blé tendre par culture de microspores isolés. Actes des VIème Journées Scientifiques du Réseau Biotechnologie-Génie génétique des plantes de l'AUPELF-UREF du 30 juin au 3 juillet 1997. Collection Université Francophones, edition ESTEM. (p. 536)

  • De Buyser J, Henry Y, Lonnet P, Hertzog R & Hespel A (1987) 'Florin' a doubled haploid wheat variety developed by anther culture method. Plant Breed. 98: 53–56

    Article  Google Scholar 

  • De Buyser J, Hachemi-Rachedi S, Lemee ML, Sejourne S, Marcotte JL & Henry Y (1992) Aneuploid analysis of anther culture response in wheat. Plant Breed. 109: 339–342

    Article  Google Scholar 

  • Ekiz A & Konzak C (1991) Nuclear and cytoplasmic control of anther culture response in wheat: II. Common wheat x alloplasmic lines. Crop Sci. 31: 1427–1431

    Article  Google Scholar 

  • Foroughi-Wher B & Zeller FJ (1990) In vitro microspore reaction of different German cultivars. Theor. Appl. Gen. 79: 77–80

    Google Scholar 

  • Gallagher L, Benyassine A, Benlhabib O & Obanni M (1987) Sources of resistance to Mayetiola destructor in bread wheat and durum wheat in Morocco. Euphytica 36: 591–602

    Article  Google Scholar 

  • Ghaemi M & Saffari A (1993) Analysis of anther culture to measure genetic variability for embryogenesis in tetraploid wheat (Triticum turgidium var. durum). J. Gen. Br. 47: 295–298

    Google Scholar 

  • Ghaemi M, Saffari A & Alibert G (1995) Influence of genotype, media composition, cold treatment and their interaction on androgenesis in durum wheat (Triticum turgidum). Cereal Res. Comm. 23: 215–222

    Google Scholar 

  • Hagemann KM, Cantrell RG & Deckard EL (1988) Tissues culture in Triticum turgidum L. var. durum. Int. Wheat Genet. Symp. 7: 741–744

    Google Scholar 

  • Henry Y & de Buyser J (1985) Effect of the 1B/1R translocation on anther culture ability in wheat (Triticum aestivum L.). Plant Cell Rep. 4: 307–310

    Article  Google Scholar 

  • Hu T & Kasha K (1997) Improvement of isolated microspore culture of wheat (Triticum aestivum L.) through ovary coculture. Plant Cell Rep. 16: 520–525

    Article  CAS  Google Scholar 

  • Jähne A & Lörz H (1995) Review article: Cereal microspore culture. Plant Sci. 109: 1–12

    Article  Google Scholar 

  • Kasha KJ & Reinberg E (1975) Utilization of haploids in barley. In: Barley Genetics III. Proceedings of the third International Barley Genet. Symp. Verlag Karl Theimig, Munich, (pp 307–317)

  • Mejza SJ, Morgant V, Di Bona DE & Wong JR (1993) Plant regeneration from isolated microspores of Triticum aestivum. Plant Cell Rep. 12: 149–153

    Article  Google Scholar 

  • Murigneux A, Bentolila S, Hardy T, Baud S, Guitton C, Jullien H, Ben Tahar S, Freyssinet G & Beckert M (1994) Genotypic variation of quantitative trait loci controlling in vitro androgenesis in maize. Genome 37: 970–976

    CAS  PubMed  Google Scholar 

  • Orlov PA, Mavrishcheva EB & Pailova AN (1993) Estimation of the response to anther culturing in 60 genotypes of different wheat species. Plant Breed. 111: 339–342

    Article  Google Scholar 

  • Otani M & Shimada T (1994) Pollen embryo formation and plant regeneration from cultured anther of tetraploid wheat. J. Genet. Br. 48: 103–106

    Google Scholar 

  • Otani M & Shimada T (1995) Effect of synthetic medium on microspore-derived embryoid formation of tetraploid wheat species. Cereal Res. Comm. 23: 345–350

    Google Scholar 

  • Pualimatka M, Laine S & Pauk J (1996) Effect of ovary cocultivation and culture medium on embryogenesis of directly isolated microspores of wheat. Cereal Res. Comm. 24: 393–400

    Google Scholar 

  • Saidi N, Cherkaoui S, Chlyah A & Chlyah A (1997) Embryo formation and regeneration in Triticcum tugidum ssp. durum. Plant Cell Tiss. Org. Cult. 51: 27–33

    Article  Google Scholar 

  • Wang P & Chen YR (1986) A study of the application of C17 medium for anther culture. Acta. Bot. Sin. 36: 237–239

    Google Scholar 

  • Ziauddin A, Marsolais A, Simmion E & Kasha KJ (1992) Improved plant regeneration from wheat anther and barley microspore culture using phenyl acetic acid (PAA). Plant Cell Rep. 11: 489–498

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Plant Physiology, Department of Biology, University Cadi Ayyad, Faculty of Sciences Semlalia, B.P. 2390, 40001, Marrakech, Morocco

    F. J'Aiti & I. El Hadrami

  2. Laboratory of Tissues Culture and Cytogenetic, Department of Agronomy, Rabat-Institutes, IAV Hassan II, B.P. 6202, 10101, Rabat, Morocco

    O. Benlhabib

  3. Department of Agronomy, Purdue University, West Lafayette, IN, USA

    H. C. Sharma

  4. Laboratoire de Biotechnologie et Amélioration des Plantes, Faculté des Sciences, 50000, Meknes, Maroc

    S. El Jaafari

Authors
  1. F. J'Aiti
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. O. Benlhabib
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. C. Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. El Jaafari
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. I. El Hadrami
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

J'Aiti, F., Benlhabib, O., Sharma, H.C. et al. Genotypic variation in anther culture and effect of ovary coculture in durum wheat. Plant Cell, Tissue and Organ Culture 59, 71–76 (1999). https://doi.org/10.1023/A:1006399512217

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006399512217

Search

Navigation