Abstract
A method is described for in vitromicropropagation through shoot apices of Acaciacatechu Willd., a semi-arid tree valued for Katha (atanin-like substance obtained from red heart wood of10–20 year old trees) and timber. Explants wereexcised from 15-days-old in vitro grownseedlings raised from superior seed stocks. Shoot budinduction from shoot apex explants was observed onMurashige and Skoog's (MS) [12] medium containingvarious growth regulators. A maximum of 12 shoots wasobtained on MS medium supplemented with 1.5 mg/l 6-benzylaminopurine (BAP) and 1.5 mg/l kinetin.Well-developed shoots (3–4 cm long) were rooted on \(\frac{1}{4}\) strength MS medium with 3.0 mg/l indole-3-acetic acid (IAA) and sucrose 1.5%. In vitro regenerated plantlets of A. catechu were transferred to field conditions.
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Badji S, Marione Y, Ndiaye I, Merlin G, Danthu P, Nevill P and Colonna JP (1993) In vitro propagation of the gum arabic tree (Acacia senegal (L.) Willd.) Developing a rapid method for producing plants. Plant Cell Rep. 12: 629-693
Barakat MN and El-Lakany MH (1992) Clonal propagation of Acacia saligna by shoot tip culture. Euphytica 59: 103-107
Barve DM and Mehta AR (1993) Clonal propagation of mature elite trees of Commiphora wightii. Plant Cell Tiss. Org. Cult. 35: 237-244
Bhati R (1988) In vitro and in vivo studies on regeneration and biochemical changes in certain tree species. Ph.D Thesis, University of Jodhpur, Jodhpur
Bonga JM and Durzan DJ (1987) Cell and Tissue Culture in Forestry. Boston: Martinus Nijhoff Pub.
Gupta P, Patni V, Kant U and Arya HC (1994) In vitro multiple shoot formation from mature trees of Acacia senegal (L.) Willd. J. Ind. Bot. Soc. 73: 331-332
Gupta PK, Fullmann G, Timmis R, Kretingen M, Carlson WC, Grob J and Welty E (1993) Forestry in the 21st Century. The biotechnology of somatic embryogenesis. Biotechnology 11: 454-459
Kaur K, Verma B and Kant U (1998) Plants obtained from Khair tree (Acacia catechu Willd.) using mature nodal segments. Plant Cell Rep. 17: 427-429
Kopp MS and Nataraja K (1991) In vitro plantlet regeneration from shoot tip cultures of Tamarindus indica L. Ind. J of Forestry 13: 30-33
Krishnan PN, Sudha CG and Seeni S (1995) Rapid propagation through shoot tip culture of Trichopus zeylanicus Gaertn., a rare ethnomedicinal plant. Plant Cell Rep. 14: 708-711
Lu CY, Harry IS, Thompson MR and Thorpe TH (1991) Plantlet regeneration from cultured embryos and seedling parts of red spruce (Picea rubens Sarg.). Bot. Gaz. 152: 42-50
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497
Nandwani D (1995) In vitro micropropagation of a tree legume adapted to arid lands: Acacia tortilis sub-sp raddiana. Ann. Des Sci. Forestieres 52: 183-189
Ostrolucka MG and Bezo M (1994 ) Utilization of meristem cultures in propagation of Oak (Quercus sp.). Genetica Polonica 35: 161-169
Rout GR, Samantary S and Das P (1995) Somatic embryogenesis and plant regeneration from callus culture of Acacia catechu-a multipurpose leguminous tree. Plant cell Tiss. Org. Cult. 42: 283-285
Ruredzo TJ and Hanson J (1993) Plant recovery from seedling-derived shoot tips of Faidherbia albida grown in vitro. Agroforestry Systems 22: 59-65
Singhal PC and Joshi LD (1984) Role of Gum arabica and Gum catechu in glycemia and Cholesterolemia. Curr. Sci. 53: 91
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Kaur, K., Kant, U. Clonal propagation of Acacia catechu Willd. by shoot tip culture. Plant Growth Regulation 31, 143–145 (2000). https://doi.org/10.1023/A:1006362318265
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DOI: https://doi.org/10.1023/A:1006362318265