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Use of Perfluorocarbon Liquids, Silicone Oil, and 5-Fluorouracil in the Management of Experimental PVR

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Abstract

Purpose: To evaluate the toxicity and efficacy of 5-fluorouracil (5-FU) in combination with perfluoroperhydrophenanthrene (Vitreon), silicone oil, or a combination of silicone oil and Vitreon in a ratio of 3:2 in the management of experimental proliferative vitreoretinopathy (PVR). Methods: Toxicity study. Seventy rabbit eyes underwent vitrectomy followed by intravitreal injection of 5-FU in doses of 800, 400, or 200 μg: Group 1, 5-FU alone; Group 2, 5-FU plus 1 mL Vitreon; Group 3, 5-FU plus 1 mL silicone oil; Group 4, 5-FU plus 0.6 mL silicone oil and 0.4 mL Vitreon; Group 5, 0.6 mL silicone oil plus 0.4 mL Vitreon. Electroretinography was performed preoperatively and 8 weeks postoperatively before the animals were sacrificed. Efficacy study. Seventy-two rabbit eyes underwent vitrectomy and were injected intravitreally with 100,000–200,000 retinal pigment epithelial cells to induce PVR. Groups were injected with 200 μg 5-FU alone or with 1 mL silicone oil, 1 mL Vitreon, or a combination of 0.6 mL silicone oil and 0.4 mL Vitreon. Others were given only 1 mL Vitreon or 1 mL silicone oil. The animals were followed for 8–12 weeks; PVR was graded using Fastenberg's system. Results: Toxicity study. Eyes given 200 μg 5-FU, silicone, and Vitreon showed mild inflammation and vitritis which resolved in 1 week; the dose was nontoxic by electroretinography and histopathology. Doses of 400 and 800 μg 5-FU were toxic. Efficacy study. Clinical severity of PVR was less in the groups which received 5-FU plus vitreous substitutes when compared to the control groups at all time points. The lowest incidences were in groups given 5-FU plus Vitreon or 5-FU plus Vitreon and silicone oil: 33.33% and 11.11%, respectively. Conclusions: A dose of 200 μg 5-FU with silicone oil and Vitreon combined was nontoxic to the rabbit retina. The combination of 5-FU, Vitreon, and silicone oil showed significant efficacy in the prevention of experimental PVR.

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Authors and Affiliations

  1. LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans, LA

    Hassan M. Hegazy, Gholam A. Peyman, Chanping Liang, Melih H. Unal, Luiz C. Molinari & Abdul A. Kazi

  2. Alazhar University, Cairo, Egypt

    Hassan M. Hegazy

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  1. Hassan M. Hegazy
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  2. Gholam A. Peyman
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  3. Chanping Liang
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Hegazy, H.M., Peyman, G.A., Liang, C. et al. Use of Perfluorocarbon Liquids, Silicone Oil, and 5-Fluorouracil in the Management of Experimental PVR. Int Ophthalmol 22, 239–246 (1998). https://doi.org/10.1023/A:1006336831730

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