Abstract
Callus cultures were initiated from axillary leaves, axillary shoots, hypocotyls, and root segments on Murashige and Skoog (MS) (1962) medium supplemented with 2,4-D (2 mg l−1) and KN (0.2 mg l−1). Shoots differentiated best from axillary shoot base callus on MS medium containing BA (2 mg l−1). Regenerated shoots rooted best on MS medium containing IBA (2 mg l−1) alone, and IBA (2 mg l−1) with IAA (2 mg l−1). Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and afterwards transferred to the glasshouse.
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Rani, G., Grover, I. In vitro callus induction and regeneration studies in Withania somnifera. Plant Cell, Tissue and Organ Culture 57, 23–27 (1999). https://doi.org/10.1023/A:1006329532561
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DOI: https://doi.org/10.1023/A:1006329532561