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Expression and cellular localization of Atrab28 during Arabidopsis embryogenesis

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Abstract

The maize abscisic acid (ABA)-responsive gene rab28 has been shown to be ABA-inducible in embryos and vegetative tissues, expression being mostly restricted to vascular elements during late embryogenesis. In the course of an expressed sequence tags (ESTs) programme, we have isolated an Arabidopsis thaliana gene, Atrab28, encoding the orthologue of maize rab28. The Atrab28 cDNA is 1090 bp long, including a poly(A)+ stretch, and encodes a polypeptide of 262 amino acids. Atrab28 antibody against the recombinant protein recognizes a polipeptide of about 30 kDa and pI 6, in close agreement with the predicted molecular mass and pI. As for maize rab28, expression studies with Atrab28 revealed high specificity for embryo tissues, transcription being stimulated by the transcriptional activator abi3. In contrast, Atrab28 was not induced in vegetative tissues by ABA, osmotic stress or dehydration. The expression of Atrab28 mRNA and the accumulation of Atrab28 protein was largely restricted to provascular tissues of mature embryos and in the seed coat outer tegument and embryo and silique epidermis, as revealed by in situ hybridization and immunocytochemistry with anti-Atrab28 antibodies.

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Authors and Affiliations

  1. Departament de Genètica Molecular, Centre d'Investigació i Desenvolupament, C.S.I.C., Jordi Girona 18-26, 08034, Barcelona, Spain

    César Arenas-Mena & Montserrat Pagès

  2. Laboratoire de Physiologie et Biologie Moléculaire des Plantes, UMR 5545 CNRS, Université de Perpignan, 66860, Perpignan, France

    Monique Raynal, Antonio Borrell, Fabrice Varoquaux & Michel Delseny

  3. Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-C.S.I.C., Camino de Vera s/n, 46022, Valencia, Spain

    Mari Cruz Cutanda, Robin A.P. Stacy & Francisco A. Culiáñez-Macià

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  1. César Arenas-Mena
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  2. Monique Raynal
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Arenas-Mena, C., Raynal, M., Borrell, A. et al. Expression and cellular localization of Atrab28 during Arabidopsis embryogenesis. Plant Mol Biol 40, 355–363 (1999). https://doi.org/10.1023/A:1006219315562

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