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Selection of Arabidopsis mutants overexpressing genes driven by the promoter of an auxin-inducible glutathione S-transferase gene

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Abstract

Transgenic arabidopsis plants were isolated that contained a T-DNA construct in which the promoter of an auxin-inducible glutathione S-transferase (GST) gene from tobacco was fused to the kanamycin resistance (nptII) as well as to the β-glucuronidase (gusA) reporter gene. Subsequently, seeds were treated with EMS to obtain mutants in which both reporter gene fusions were up-regulated. Northern analysis showed that the mRNA level of a related, endogenous auxin-inducible GST gene of Arabidopsis was increased in some of these mutants as well. Two of the gup (GST up-regulated) mutants were characterized in more detail and roughly mapped. Both had epinastic cotyledons and leaves, a phenotype that turned out to be linked to the gup mutation.

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Author notes

  1. Dianne A.M. van der Kop

    Present address: ATO-DLO, Bornsesteeg 58, 6708 PD, Wageningen, Netherlands

Authors and Affiliations

  1. Institute of Molecular Plant Sciences and RUL-TNO Centre for Phytotechnology, Clusius Laboratory, Leiden University, Wassenaarseweg 64, 2333 AL, Leiden, Netherlands

    Monique Schuyer, Johan E. Pinas, Bert J. van der Zaal & Paul J.J. Hooykaas

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  1. Dianne A.M. van der Kop
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  2. Monique Schuyer
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van der Kop, D.A., Schuyer, M., Pinas, J.E. et al. Selection of Arabidopsis mutants overexpressing genes driven by the promoter of an auxin-inducible glutathione S-transferase gene. Plant Mol Biol 39, 979–990 (1999). https://doi.org/10.1023/A:1006129426712

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