Skip to main content
SpringerLink
Log in

Storage of potato microplants in vitro in the presence of acetylsalicylic acid

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Acetylsalicylic acid was investigated as an alternative medium supplement to mannitol for slow-growth in vitro storage of potato microplants. At 8°C, culture in the presence of either 100 μM acetylsalicylic acid or 4% mannitolretarded microplant stem growth, and required intervals between subcultures ranged from 8 to over 12 months, depending on the genotype. Several media were tested for recovery of clones from slow-growth storage, with similar efficiences of recovery achieved for microplants from either mannitol or acetylsalicylic acid media. Prolonged culture on acetylsalicylic acid had no adverse effects on the yield of minitubers on plantlets transferred to glasshouse cultivation. At 18°C also, intervals between subcultures could be prolonged to 5.5–6 months by culture on either mannitol or acetylsalicylic acid media. Microplants cultured on acetylsalicylic acid did not exhibit the frequent phenotypic abnormalities found in microplants on mannitol medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bessembinder JJE, Staritsky G & Zandvoort EA (1993) Long-term in vitro storage of Colocasia esculenta under minimal growth conditions. Plant Cell Tiss. Org. Cult. 33: 121–127

    Article  Google Scholar 

  • Compton ME (1994) Statistical methods for the analysis of plant tissue culture data. Plant Cell Tiss. Org. Cult. 37: 217–242

    Google Scholar 

  • Davies WJ & Jones HG (1991) Abscisic Acid. Bios Scientific, Oxford

  • Dodds JH, Huaman Z & Lizarraga R (1991) Potato germplasm conservation. In: JH Dodds (ed) In Vitro Methods for Conservation of Plant Genetic Resources (pp 93–109). Chapman and Hall, London

    Google Scholar 

  • Engelmann F (1991) In vitro conservation of tropical plant germplasm — a review. Euphytica 57: 227–243

    Article  Google Scholar 

  • Espinoza N, Estrada R, Tovar P, Bryan J & Dodds JH (1984) Tissue culture micropropagation, conservation, and export of potato germplasm. Specialized Technology Document, International Potato Center, Lima, Peru

    Google Scholar 

  • Espinoza NO, Estrada R, Silva-Rodriguez D, Tovar P, Lizarraga R & Dodds JH (1986) The potato: a model crop plant for tissue culture. Outlook Agric. 15: 21–26

    Google Scholar 

  • Harding K (1991) Molecular stability of the ribosomal RNA genes in Solanum tuberosum plants recovered from slow growth and cryopreservation. Euphytica 55: 141–146

    Article  CAS  Google Scholar 

  • Harding K (1994) The methylation status of DNA derived from potato plants recovered from slow growth. Plant Cell Tiss. Org. Cult. 37: 31–38

    Article  CAS  Google Scholar 

  • Jarret RL & Gawel N (1991) Chemical and environmental growth regulation of sweetpotato (Ipomoea batatas (L.) Lam.) in vitro. Plant Cell Tiss. Org. Cult. 25: 153–159

    CAS  Google Scholar 

  • Lizarraga R, Huaman Z & Dodds JH (1989) In vitro conservation of potato germplasm at the International Potato Center. Am. Potato J. 66: 253–269

    Google Scholar 

  • Lizarraga R, Panta A, Jayasinghe U & Dodds J (1991) Cultivo de tejidos para la eliminacion de patogenos. CIP Research Guide 3, International Potato Center, Lima, Peru

    Google Scholar 

  • Lopez-Delgado H (1993) Micropropagacion de papa empleando acido acetilsalicilico. Annual Report, Potato Program, Instituto Nacional de Investigaciones Forestales y Agropecuarias (INIFAP)-CIRCE, Toluca, Mexico

  • Lopez-Delgado H & Carrillo-Castañeda G (1996) Acetylsalicylic acid: its effects on a highly expressed phosphatase from Solanum cardiophyllum. Biotecnol. Apl. 13: 186–189

    CAS  Google Scholar 

  • Lopez-Delgado H & Scott IM (1997) Induction of in vitro tuberization of potato microplants by acetylsalicylic acid. J. Plant Physiol. 151: 74–78

    CAS  Google Scholar 

  • Lopez-Delgado H, Dat JF, Foyer CH & Scott IM (1998) Induction of thermotolerance in potato microplants by acetylsalicylic acid and H2O2. J. Exp. Bot. 49: 713–720

    Article  CAS  Google Scholar 

  • Lopez-Delgado H, Lopez-Peralta CG, Villalobos-Arambula VM & Larque-Saavedra A (1990) Efecto del acido acetil salicilico en el crecimiento de yemas de Solanum cardiophyllum cultivadas in vitro. Agroci. Ser. Fitoci. 1: 145–159

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant. 15: 437–496

    Article  Google Scholar 

  • Ng SYC & Ng NQ (1991) Reduced-growth storage of germplasm. In: JH Dodds (ed) In Vitro Methods for Conservation of Plant Genetic Resources (pp 11–39). Chapman and Hall, London

    Google Scholar 

  • Niederhauser JS (1993) International cooperation in potato research and development. Annu. Rev. Phytopathol. 31: 1–21

    Article  Google Scholar 

  • Pierpoint WS (1994) Salicylic acid and its derivatives in plants: medicines, metabolites and messenger molecules. Adv. Bot. Res. 20: 165–235

    Google Scholar 

  • Potter RH & Jones MGK (1991) Molecular analysis of genetic stability. In: JH Dodds (ed) In Vitro Methods for Conservation of Plant Genetic Resources (pp 71–91). Chapman and Hall, London

    Google Scholar 

  • Roca WM, Chavez R, Martin ML, Arias DI, Mafla G & Reyes R (1989) In vitro methods of germ-plasm conservation. Genome 31: 813–817

    Google Scholar 

  • Ryals JA, Neuenschwander UH, Willits MG, Molina A, Steiner HY & Hunt MD (1996) Systemic acquired resistance. Plant Cell 8: 1809–1819

    Article  PubMed  CAS  Google Scholar 

  • Scott IM & Yamamoto H (1994) Mass spectrometric quantification of salicylic acid in plant tissues. Phytochemistry 37: 335–336

    Article  CAS  Google Scholar 

  • Voesenek LACJ & Vanderveen R (1994) The role of phytohormones in plant stress — too much or too little water. Acta Bot. Neerl. 43: 91–127

    CAS  Google Scholar 

  • Watanabe KN, Valkonen JPT & Gregory P (1995) Use of plant biotechnology tools in plant protection, genetic resources management and crop genetic improvement: an interdisciplinary approach with potatoes at the International Potato Center. In: DW Altman & KN Watanabe (eds) Plant Biotechnology Transfer to Developing Countries. (pp 179–190). RG Landes Co., Austin, Texas

    Google Scholar 

  • Westcott RJ (1981) Tissue culture storage of potato germplasm. 2. Use of growth retardants. Potato Res. 24: 343–352

    Article  CAS  Google Scholar 

  • Withers LA, Wheelans SK & Williams JT (1990) In vitro conservation of crop germplasm and the IBPGR databases. Euphytica 45: 9–22

    Google Scholar 

  • Yalpani N, Enyedi AJ, León J & Raskin I (1994) Ultraviolet light and ozone stimulate accumulation of salicylic acid, pathogenesis-related proteins and virus resistance in tobacco. Planta 193: 372–376

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Programa de Papa, Instituto Nacional de Investigaciones Forestales y Agropecuarias (INIFAP), Metepec 2, Mex. 52142, A.P.10-2, Mexico

    H. Lopez-Delgado & M. Jimenez-Casas

  2. Institute of Biological Sciences, University of Wales, Aberystwyth, SY23 3DA, UK

    H. Lopez-Delgado & I.M. Scott

Authors
  1. H. Lopez-Delgado
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Jimenez-Casas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I.M. Scott
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lopez-Delgado, H., Jimenez-Casas, M. & Scott, I. Storage of potato microplants in vitro in the presence of acetylsalicylic acid. Plant Cell, Tissue and Organ Culture 54, 145–152 (1998). https://doi.org/10.1023/A:1006110118669

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006110118669

Search

Navigation