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In vitro stem elongation of sweet pepper in media containing 24-epi-brassinolide

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Abstract

In vitro regeneration of sweet pepper (Capsicum annuum L. cvs Jupiter and Pimiento Perfection) has been performed via direct organogenesis. The resulting shoot-buds were placed on media containing 24-epi-brassinolide (EBR) 0.1 μM, a plant steroid lactone, in the presence or absence of zeatin 9.1 μM plus GA3 5.2 μM for further stem elongation. Different responses to these treatments were recorded depending upon the protocols used and the genotypes tested. It appears that EBR does not always act directly on stem elongation but may be an elicitor and/or an enhancer of elongation in concert with endogenous and other exogenously added growth regulators. Elongated shoots were easily rooted with alpha-naphtalenacetic acid 0.5 μM (0.1 mgl-1) and transfered to soil, and following acclimation were taken to maturity in the greenhouse.

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Author notes

  1. Martine Franck-Duchenne & Sofia Ben Tahar

    Present address: Service de Neurovirologie, CEA, CENFAR, BP6, 60-68 Avenue de la Division Leclerc, 92265, Fontenay aux Roses, France)

Authors and Affiliations

  1. Biocem, 24 Avenue des Landais, 63170, Aubiére, France

    Martine Franck-Duchenne & Sofia Ben Tahar

  2. Department of Cell Biology, The Scripps Research Institute, BCC 206, 10550 North Torrey Pines Rd, La Jolla, Ca, 92037, USA

    Martine Franck-Duchenne, Yuwen Wang & Roger N. Beachy

Authors
  1. Martine Franck-Duchenne
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  2. Yuwen Wang
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  3. Sofia Ben Tahar
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  4. Roger N. Beachy
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Franck-Duchenne, M., Wang, Y., Ben Tahar, S. et al. In vitro stem elongation of sweet pepper in media containing 24-epi-brassinolide. Plant Cell, Tissue and Organ Culture 53, 79–84 (1998). https://doi.org/10.1023/A:1006071803855

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  • DOI: https://doi.org/10.1023/A:1006071803855

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