Abstract
We investigated the transmission of Borrelia garinii and Borrelia afzelii between male and female Ixodes persulcatus ticks. For this purpose the infection rate of partners from tick couples was determined by polymerase chain reaction and reverse line blot. In couples, where the male tick was infected with B. garinii, four out of nine female partners carried B. garinii. In eight couples, male ticks had a dual infection of B. afzelii and B. garinii and three female partners were infected by Borrelia spirochetes. Two female ticks carried B. garinii, and one female tick had a dual infection. No evidence for transmission of B. afzelii from male to female ticks was found among seven couples. In 45 couples where the female tick was infected, not one male tick carried spirochetes. The difference in the B. garinii infection rate between male and female ticks among these couples is highly significant. Our data suggest that transmission of B. garinii from male ticks to female ticks does occur. Sexual transmission of this pathogen may play an important role in the maintenance of B. garinii in I. persulcatus. © Rapid Science Ltd. 1998
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REFERENCES
Alekseev, A.N. and Dubinina, H.V. 1996a. Exchange of Borrelia burgdorferi between Ixodes persulcatus (Ixodidae, Acarina) sexual partners. J. Med. Entomol. 33: 351-354.
Alekseev, A.N. and Dubinina, H.V. 1996b. Venereal and cannibalistic way of Borrelia burgdorferi sensu lato exchange between males and females of Ixodes persulcatus (Ixodidae, Acarina). Roczniki Akad. Med. Bialystoku. 41: 103-110.
Alekseev, A.N., Dubinina, H.V., Antykova, L.P., Dzhivanyan, T.I., Rijpkema, S.G.T., Verbeek-De Kruif, N. and Cinco, M. 1998. Tick-borne borrelioses pathogen identification in Ixodes ticks (Acarina, Ixodidae) collected in St Petersburg and Kaliningrad Baltic Regions of Russia. J. Med. Entomol. 35: 136-142.
Chunikhin, S.P., Stefutkina, L.F., Korolev, M.B., Reshetnikov, I.A. and Khozinskaya, G.A. 1983. [Sexual transmission of tick-borne encephalitis virus in ixodids (Ixodidae).] Parazitologia 17: 214-217 (in Russian).
Gaber, M.S., Khalil, G.M. and Hoogstraal, H. 1982. Borrelia crocidurae: venereal transfer in Egyptian Ornithodorus erraticus ticks. Exp. Parasitol. 54: 182-184.
Gaber, M.S., Khalil, G.M., Hoogstraal, H. and Aboul-Nasr, A.E. 1984. Borrelia crocidurae localization and transmission in Ornithodoros erraticus and O. savignyi. Parasitology 88: 403-413.
Kondrashova, Z.N. 1975. [Materials for the tick-borne encephalitis ecology (experimental investigation of Ixodes persulcatus P. Sch. Ticks as a medium of their existence]. Avtoreferat dissertation, Doctor of Science, Moscow (in Russian).
Korenberg, E.I., Gorelova, N.B., Postic, D., Kovalevsky, Yu.V., Baranton, G. and Vorobyeva, N.N. 1997. [Reservoir hosts and vectors of Borrelia, causative agents of ixodid tick-borne borrelioses in Russia.] Zh. Mikrobiol. (Moscow) 6: 36-38 (in Russian).
Lesnyak, O., Laikovskaya, E., Kufko, I., Bruinink, H., Baranova, N. and Rijpkema, S. 1998. Clinical features of Lyme borreliosis in the Middle Urals and the distribution of Borrelia burgdorferi sensu lato species in local Ixodes persulcatus ticks. Zbl. Bakteriol. in press.
Moskvitina, G.G., Korenberg, E.I., Spielman, A. and Schhyogolova, T.V. 1995. [On frequencies of generalized infection in unfed adult ticks of the genus Ixodes in Russian and American foci of the borrelioses.] Parazitologia 29: 353-360 (in Russian).
Postic, D., Assous, M., Grimont, P.A.D. and Baranton, G. 1994. Diversity of Borrelia burgdorferi as a tool for classification. Zbl. Bakteriol. 277: 28-33.
Postic, D., Korenberg, E.I., Gorelova, N., Kovalevski, Y.V., Bellenger, E. and Baranton, G. 1997. Borrelia burgdorferi sensu lato in Russia and neighbouring countries: high incidence of mixed isolates. Res. Microbiol. 148: 691-702.
Rijpkema, S.G.T., Molkenboer, M.J.C.H., Schouls, L.M., Jongejan, F. and Schellekens, J.F.P. 1995. Simultaneous detection and genotyping of three genomic groups of Borrelia burgdorferi sensu lato in Dutch Ixodes ricinus ticks by characterization of the amplified intergenic spacer region between 5S and 23S rRNA genes. J. Clin. Microbiol. 33: 3091-3095.
Shashina, N.I. 1985. [Total duration of life cycle.] In Taiga tick Ixodes persulcatus Schulze (Acarina, Ixodidae), N.A. Filippova (ed.), pp. 275-277. Nauka. Leningrad. (in Russian).
Stefutkina, L.F. 1989. [Morphological and virological peculiarities of the tissue and cell infection of ixodid ticks by the tick-borne encephalitis virus.] Avtoreferat diss. PhD, Moscow. (in Russian).
Van Dam, A.P., Kuiper, H., Vos, K., Widjojokusumo, A., de Jongh, B.M., Spanjaard, L., Ramselaar, A.C., Kramer, M.D. and Dankert, J. 1993. Different genospecies of Borrelia burgdorferi are associated with distinct clinical manifestation of Lyme borreliosis. Clin. Infect. Dis. 17: 708-717.
Wagner-Jevseenko, O. 1958. Fortplanzung bei Ornithodoros moubata und genitale Ubertragung von Borrelia duttoni. Acta Trop. 15: 118-168.
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Alekseev, A.N., Dubinina, H.V., Rijpkema, S.G. et al. Sexual transmission of Borrelia garinii by male Ixodes persulcatus ticks (Acari, Ixodidae). Exp Appl Acarol 23, 165–169 (1999). https://doi.org/10.1023/A:1006058728821
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DOI: https://doi.org/10.1023/A:1006058728821