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The light-regulated Arabidopsis bZIP transcription factor gene ATB2 encodes a protein with an unusually long leucine zipper domain

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Abstract

A light-regulated basic domain/leucine zipper gene, ATB2, was identified in an Arabidopsis thaliana transcription factor gene collection. Both genomic and cDNA clones of ATB2 were isolated. The gene encodes a small protein (18 kDa) which mainly consists of the basic domain and an unusually long leucine zipper. The expression of the ATB2 gene is induced when etiolated or dark-adapted seedlings are transferred to the light. Moreover, its expression is derepressed in dark-grown seedlings of the photomorphogenic mutants cop1 and det1. In mature plants, transcript levels are particularly high in flowers and also light-responsive in these tissues.

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References

  1. Alber T: Stucture of the leucine zipper. Curr Op Gen Devel 2: 205–210 (1992).

    Google Scholar 

  2. Bowler C, Neuhaus G, Yamagata H, Chua N.-H: Cyclic GMP and calcium mediate phytochrome phototransduction. Cell 77: 73–81 (1994).

    Google Scholar 

  3. Castresana C, Garcia-Luque I, Alonso E, Malik VS, Cashmore AR: Both positive and negative regulatory elements mediate expression of a photoregulated CAB gene from Nicotiana plumbaginifolia. EMBO J 7: 1929–1936 (1988).

    Google Scholar 

  4. Chory J: Out of darkness: mutants reveal pathways controlling lightregulated development in plants. Trends Genet 9: 167–172 (1993).

    Google Scholar 

  5. Chory J, Peto C, Feinbaum R, Pratt L, Ausubel F: Arabidopsis thaliana mutant that develops as a light-grown plant in the absence of light. Cell 58: 991–999 (1989).

    Google Scholar 

  6. Church GM, Gilbert W: Genomic sequencing. Proc Natl Acad Sci USA 81: 1991–1995 (1984).

    Google Scholar 

  7. Cseplö A, Etzold T, Schell J, Schreier PH: Point mutations in the 23S rRNA genes of four lincomycin resistant Nicotiana plumbaginifolia mutants could provide new selectable markers for chloroplast transformation. Mol Gen Genet 214: 295–299 (1988).

    Google Scholar 

  8. Deng X-W, Caspar T, Quail PH: Cop1: a regulatory locus involved in lightcontrolled development and gene expression in Arabidopsis. Genes Devel 5: 1172–1182 (1991).

    Google Scholar 

  9. Deng X-W, Matsui M, Wei N, Wagner D, Chu AM, Feldmann KA, Quail PH: COP1, anArabidopsis regulatory gene, encodes a protein with both a zincbinding motif and a Gb homologous domain. Cell 71: 791–801 (1992).

    Google Scholar 

  10. Feldman LJ, Piechulla B, Sun PS: Light-regulated protein and mRNA synthesis in root caps of maize. Plant Mol Biol 11: 27–34 (1988).

    Google Scholar 

  11. Furuya M: Phytochromes: their molecular species, gene families, and functions. Annu Rev Plant Physiol Plant Mol Biol 44: 617–645 (1993).

    Google Scholar 

  12. Geballe AP, Morris DR: Initiation codons within 5′ leaders of mRNAs as regulators of translation. Trends Biochem Sci 19: 159–164 (1994).

    Google Scholar 

  13. Giuliano G, Pichersky E, Malik VS, Timko MP, Scolnik PA, Cashmore AR: An evolutionarily conserved protein binding sequence upstream of a plant lightregulated gene. Proc Natl Acad Sci USA 85: 7089–7093 (1988).

    Google Scholar 

  14. Guiltinan MJ, Marcotte WR, Quatrano RS: Aplant leucine zipper protein that recognizes an abscisic acid response element. Science 250: 267–271 (1990).

    Google Scholar 

  15. Izawa T, Foster R, Nakajima M, Shimamoto K, Chua N-H: The rice bZIP transcriptional activator RITA-1 is highly expressed during seed development. Plant Cell 6: 1277–1287 (1994).

    Google Scholar 

  16. Katagiri F, Lam E, Chua N-H: Two tobacco DNAbinding proteins with homology to the nuclear factor CREB. Nature 340: 727–730 (1989).

    Google Scholar 

  17. Kendrick RE, Kronenberg GHM (eds): Photomorphogenesis in Plants. Kluwer Academic Publishers, Dordrecht, Netherlands (1994).

    Google Scholar 

  18. Krebbers E, Seurinck J, Herdies L, Cashmore AR, Timko MP: Four genes in two diverged subfamilies encode the ribulose1,5-biphosphate carboxylase small subunit polypeptides of Arabidopsis thaliana. Plant Mol Biol 11: 745–759 (1988).

    Google Scholar 

  19. Leutwiler LS, Meyerowitz EM, Tobin EM: Structure and expression of three light-harvesting chlorophyll a/bbinding protein genes from Arabidopsis thaliana. Nucl Acids Res 14: 4051–4064 (1986).

    Google Scholar 

  20. Lohmer S, Maddaloni M, Motto M, Salamini F, Thompson RD: Translation of the mRNA of the maize transcriptional activator Opaque-2 is inhibited by upstreamopen reading frames present in the leader sequence. Plant Cell 5: 65–73 (1993).

    Google Scholar 

  21. Lupas A, Van Dyke M, Stock J: Predicting coiled coils from protein sequences. Science 252: 1162–1164 (1991).

    Google Scholar 

  22. McKendree WL, Ferl RJ: Functional elements of the Arabidopsis Adh promoter include the G-box. Plant Mol Biol 19: 859–862 (1992).

    Google Scholar 

  23. McNellis TW, Deng X-W: Light control of seedling morphogenetic pattern. Plant Cell 7: 1749–1761 (1995).

    Google Scholar 

  24. McNellis TW, von Arnim AG, Deng X-W: Overexpression of Arabidopsis COP1 results in partial suppression of lightmediated development: evidence for a lightinactivable repressor of photomorphogenesis. Plant Cell 6: 1391–1400 (1994).

    Google Scholar 

  25. Menkens AE, Cashmore AR: Isolation and characterization of a fourth Arabidopsis thaliana G-boxbinding factor, which has similarities to Fos oncoprotein. Proc Natl Acad Sci USA 91: 2522–2526 (1994).

    Google Scholar 

  26. Millar AJ, McGrath RB, Chua N-H: Phytochrome phototransduction pathways. Annu Rev Genet 28: 325–349 (1994).

    Google Scholar 

  27. Nakagawa H, Ohmiya K, Hattori T: A rice bZIP protein, designated OSBZ8, is rapidly induced by abscisic acid. Plant J 9: 217–227 (1996).

    Google Scholar 

  28. Neuhaus G, Bowler C, Kern R, Chua NH: Calcium/ calmodulindependent and independent phytochrome signal transduction pathways. Cell 73: 937–952 (1993).

    Google Scholar 

  29. Oelmüller R: Photooxidative destruction of chloroplasts and its effect on nuclear gene expression and extraplastidic enzyme levels. Photochem Photobiol 49: 229–239 (1989).

    Google Scholar 

  30. Pabo CO, Sauer RT: Transcription factors: Structural families and principles of DNA recognition. Annu Rev Biochem 61: 1053–1095 (1992).

    Google Scholar 

  31. Pepper A, Delaney T, Washburn T, Poole D, Chory J: DET1, a negative regulator of light-mediated development and gene expression in Arabidopsis, encodes a novel nuclearlocalized protein. Cell 78: 109–116 (1994).

    Google Scholar 

  32. Pruitt RE, Meyerowitz EM: Characterization of the genome of Arabidopsis thaliana. J Mol Biol 187: 169–183 (1986).

    Google Scholar 

  33. Quaedvlieg N, Dockx J, Rook F, Weisbeek P, Smeekens S: The homeobox gene ATH1 of Arabidopsis is derepressed in the photomorphogenic mutants cop1 and det1. Plant Cell 7: 117–129 (1995).

    Google Scholar 

  34. Quaedvlieg N, Dockx J, Keultjes G, Kock P, Wilmering J, Weisbeek P, Smeekens S: Identification of a light-regulated MYB gene from an Arabidopsis transcription factor gene collection. Plant Mol Biol 32: 987–993 (1996).

    Google Scholar 

  35. Schindler U, Menkens AE, Beckmann H, Ecker JR, Cashmore AR: Heterodimerization between light-regulated and ubiquitously expressed Arabidopsis GBF bZIP proteins. EMBO J 11: 1261–1273 (1992).

    Google Scholar 

  36. Schmidt RJ, Burr FA, Aukerman MJ, Burr B: Maize regulatory gene opaque-2 encodes a protein with a ‘leucine-zipper’ motif that binds to zein DNA. Proc Natl Acad Sci USA 87: 46–50 (1990).

    Google Scholar 

  37. Taylor WC: Regulatory interactions between nuclear and plastid genomes. Annu Rev Plant Physiol Plant Mol Biol 40: 211–233 (1989).

    Google Scholar 

  38. Theill LE, Castrillo J-L, Wu D, Karin M: Dissection of functional domains of the pituitaryspecific transcription factor GHF-1. Nature 342: 945–948 (1989).

    Google Scholar 

  39. van der Krol AR, Chua N-H: The basic domain of plant BZIP proteins facilitates import of a reporter protein into plant nuclei. Plant Cell 3: 667–675 (1991).

    Google Scholar 

  40. Varagona MJ, Schmidt RJ, Raikhel NV: Nuclear localization signal(s) required for nuclear targeting of the maize regulatory protein Opaque-2. Plant Cell 4: 1213–1227 (1992).

    Google Scholar 

  41. Vorst O, Kock P, Lever A, Weterings B, Weisbeek P, Smeekens S: The promoter of the Arabidopsis thaliana plastocyanin gene contains a far upstream enhancerlike element involved in chloroplastdependent expression. Plant J 4: 933–945 (1993).

    Google Scholar 

  42. Weisshaar B, Armstrong GA, Block A, da Costa e Silva O, Hahlbrock K: Light-inducible and constitutively expressed DNAbinding proteins recognizing a plant promoter element with functional relevance in light reponsiveness. EMBO J 10: 1777–1786 (1991).

    Google Scholar 

  43. Williams ME, Foster R, Chua NH: Sequences flanking the hexameric G-box core CACGTG affect the specificity of protein binding. Plant Cell 4: 485–496 (1992).

    Google Scholar 

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  1. Fred Rook

    Present address: Department of Molecular Genetics, John Innes Centre, Colney, Norwich, NR4 7UH, UK

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  1. Department of Molecular Genetics, John Innes Centre, Colney, Norwich, NR4 7UH, UK

    Peter Weisbeek & Sjef Smeekens

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  1. Fred Rook
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  2. Peter Weisbeek
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  3. Sjef Smeekens
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Rook, F., Weisbeek, P. & Smeekens, S. The light-regulated Arabidopsis bZIP transcription factor gene ATB2 encodes a protein with an unusually long leucine zipper domain. Plant Mol Biol 37, 171–178 (1998). https://doi.org/10.1023/A:1005964327725

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  • DOI: https://doi.org/10.1023/A:1005964327725

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