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Cloning and characterisation of MEK1, an Arabidopsis gene encoding a homologue of MAP kinase kinase

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Abstract

We report the cloning of a cDNA for MEK1, an Arabidopsis thaliana gene encoding a homologue of MAP kinase kinase (MEK). The predicted protein sequence shows 41% identity over 270 amino acids to vertebrate MEK proteins, and contains conserved features characteristic of MEK. Analysis of transcript levels show that expression of the gene is regulated by developmental processes (etiolation/de-etiolation) and by wounding. However in contrast to the rapid wound induction of MAP kinase transcripts in other plant species, MEK1 transcripts first accumulated 6–12 h after wounding.

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Authors and Affiliations

  1. Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh, EH14 4AS, UK

    Peter C. Morris

  2. Institut des Sciences Végétales, Centre National de la Recherche Scientifique UPR 40, Avenue de la Terrasse, 91198, Gif sur Yvette Cedex, France

    Danièle Guerrier, Jeff Leung & Jérôme Giraudat

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  1. Peter C. Morris
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  2. Danièle Guerrier
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  3. Jeff Leung
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  4. Jérôme Giraudat
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Morris, P.C., Guerrier, D., Leung, J. et al. Cloning and characterisation of MEK1, an Arabidopsis gene encoding a homologue of MAP kinase kinase. Plant Mol Biol 35, 1057–1064 (1997). https://doi.org/10.1023/A:1005963222768

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